Feed & Additive Magazine Issue 54 July 2025

July 2025 Year: 5 Issue: 54 Feed Additive INTERNATIONAL MAGAZINE FOR ANIMAL FEED & ADDITIVES INDUSTRY Dr. Xiaoli Dong, CJ BIO Enhancing growth, immunity, and heat stress resilience Dr. Dejan Miladinovic, NMBU Why AI-driven predictive maintenance for feed mills is a game-changer William Woodley, Woodley Dairy Direction Redefining forage particle size for ruminants www.feedandadditive.com Global Grain Supply and Demand Set to Break Records Heat Stress Management

Publisher Muhammed Akatay akatay@feedandadditive.com Responsible Manager Özlem Akatay ozlem@feedandadditive.com Editor-in-Chief Derya Yıldız derya@feedandadditive.com Editors Burak Varol Zeynep Demirkaya editor@feedandadditive.com Communication & Marketing Lisa Atakul marketing@feedandadditive.com Design Alper Kandemir graphic@feedandadditive.com Web Design Caner Cem Pulat web@threeplusmedia.com Circulation and Data Responsible Amine Şengün sm@threeplusmedia.com Ataturk St. Metropol Istanbul Ste. Block C1, No:376 Atasehir, Istanbul/TURKEY Phone: +90 850 220 5064 E-Mail: info@feedandadditive.com Web: www.feedandadditive.com Publication Type: Periodic (Monthly) EDITOR Dear Readers, As we feel the sun on our faces more with each passing day, we turn our gazes to our animals, who are feeling the effects of summer far more intensely than us. In this July issue of Feed & Additive Magazine, we focus on the urgent and growing challenge of heat stress management in farm animals; a concern that continues to escalate under the intensifying effects of climate change. From rising ambient temperatures and humidity to poor ventilation and housing conditions, animals are increasingly exposed to environments that compromise their health, welfare, and productivity. Heat stress doesn’t just mean discomfort; it can and does also lead to reduced feed intake, impaired fertility, lowered immune response, and even mortality in severe cases. On this hot July day, we examine the multifactorial causes of heat stress, its wide-ranging impacts, and most importantly, the innovative and holistic solutions being explored to mitigate its effects. Our contributors highlight climate-smart strategies and the evolving role of nutrition and feed additives in supporting productivity and resilience in livestock. As temperatures rise globally, effective heat stress management is no longer optional; it is essential. We hope this issue serves as both a timely resource and a call to action for producers, researchers, and industry professionals alike. We will see you in the next issue. In the meantime, stay cool and stay informed. Enjoy your reading! From Sunlight to Stress: The Growing Challenge of Heat in Animal Farming Derya YILDIZ © Three Plus Media | All rights reserved. Reproduction, in whole or in part, is prohibited without the written permission of the publisher. *DISCLAIMER: All views and ideas expressed in articles published in the magazine reflect the point of views of the author(s), not that of the Editor-in-Chief, Editors, Editorial Board or Publisher of Feed & Additive Magazine. The Editor-in-Chief, Editors, Editorial Board and Publisher assume no responsibility or liability in such cases. The publisher does not give any warranty and representation to the accuracy or completeness of the contents and data published in the magazine. All responsibility of the contents, articles and information published in the magazine belongs to the authors. The publisher and the authors cannot be held responsible for any damages resulting from the use of the whole or part of the content of the magazine. feedandadditive

Climate change means taking preventative strategies to mitigate heat stress are essential. With Selko’s Heat Stress Programme we offer feed additive strategies that can support animal health and production during heat stress conditions and increase productivity, reduce infectious diseases and mortality rates. THE BENEFITS HEAT STRESS Reduce oxidative stress Increase osmoregulation interventions Optimize gut health and function Increase animal wellbeing and performance Don’t let heat stress burn through your profits Learn more, visit www.selko.com

CON T E N T S 24 ISSUE FOCUS HEAT STRESS MANAGEMENT 36 ARGININE IN BROILERS: ENHANCING GROWTH, IMMUNITY, AND HEAT STRESS RESILIENCE Dr. Xiaoli Dong, CJ BIO China 58 MANAGING HEAT STRESS IN POULTRY: THE ROLE OF OXIDATIVE STRESS AND GUT HEALTH By Technical Team of EW Nutrition 72 MANAGING HEAT STRESS IN SWINE AND RUMINANTS: NEW INSIGHTS AND NUTRITIONAL STRATEGIES By CCPA 40 DAIRY COW HEALTH UNDER HEAT STRESS: THE ROLE OF ISOQUINOLINE ALKALOIDS Lucas Queiroz & Julia Schmitt, Phytobiotics Futterzusatzstoffe 64 MANAGING HEAT STRESS AND ENDOTOXIN THREATS IN MODERN DAIRY FARMING Kurt Van De Mierop, Nutrex 74 A NOVEL NUTRITIONAL SOLUTION TO MITIGATE HEAT STRESS IN DAIRY COWS Dr. Stephanie Ladirat, Nuqo Feed Additives 44 HEAT STRESS IN DAIRY COWS: 4 EVIDENCE-BASED REASONS TO RETHINK YOUR MINERAL STRATEGY Gavin Boerboom, Trouw Nutrition 68 HEAT STRESS IN PIGS: WHY IT MATTERS AND HOW TO MANAGE IT Mathieu Cortyl, Tonisity International 78 SUPPORTING POULTRY DURING HEAT STRESS: RISKS AND TECHNICAL SOLUTIONS By Phosphea 26 FEED FORMULATION STRATEGIES TO COMBAT HEAT STRESS IN LACTATING COWS Aurélien Piron, Lallemand Animal Nutrition 48 THERMAL STRESS IN COWS: UNDERSTANDING THE METABOLIC BURDEN Dr. Melinda Culver, Biochem 31 PRESERVING GUT INTEGRITY AND REDUCING ENTERITIS RISK IN BROILERS UNDER HEAT STRESS Dr. Alain Riggi & Lin Wang, Phileo by Lesaffre 53 OLIVE BIOACTIVES AS A STRATEGY TO IMPROVE ANIMAL INTESTINAL INTEGRITY Dr. Jose J. Pastor, Lucta

CON T E N T S 81 OPTIMIZED FEEDING IS KEY TO DAIRY FARM PROFITABILITY & SUSTAINABILITY Fernando Mazeris, Dairy Data Warehouse B.V 84 WHY AI-DRIVEN PREDICTIVE MAINTENANCE FOR FEED MILLS IS A GAME-CHANGER Dr. Dejan Miladinovic, Norwegian University of Life Sciences 88 SMART MONITORING IN FEED PRODUCTION: A NEW ERA OF SAFETY AND SUSTAINABILITY Barry Finnegan, LvLogics TECHNOLOGY 92 REDEFINING FORAGE PARTICLE SIZE FOR RUMINANTS William Woodley, Woodley Dairy Direction ARTICLE MARKET REPORT GLOBAL GRAIN SUPPLY AND DEMAND SET TO BREAK RECORDS IN NEW SEASON 98 Derya Yildiz, Feed & Additive Magazine dsm-firmenich introduces postbiotic additive for piglets 16 Asia’s livestock and feed technologies to take centre stage in Taiwan 119 Bühler opens Milling Academy for food and feed milling professionals 19 Evonik to continue optimizing its global methionine production sites 10 Volare secures €26M to build protein plant 106 Kemin receives pig gut health solution approval from EU 22 Olymel to enhance processing plant with new pork and poultry products 103 Alltech becomes majority owner of Alltech Fennoaqua 14 South Korea launches first land-based salmon farm with RAS technology 112 Global fishmeal production rises despite regional declines 13 Cargill strengthens feed production with new partnership in Philippines 110 NEWS

6 FEED & ADDITIVE MAGAZINE July 2025 In the complex world of animal nutrition, minerals play a vital, yet often underappreciated, role. From sustaining animal health to supporting productivity, macro-minerals and trace elements are indispensable in livestock diets. At the heart of advocating for these essential ingredients in Europe is EMFEMA — the European Manufacturers of Feed Minerals Association. Founded in 1996, EMFEMA occupies a unique position as the only EU-wide organization focusing specifically on mineral products within the feed sector. Minerals are ever-present in animal feed, whether in trace amounts or as major dietary components. Despite their ubiquity, they are often overlooked in both policy discussions and public perception. EMFEMA seeks to remedy this oversight by advocating for a regulatory environment that supports safe, sustainable, and economically viable mineral use. Acting as the “feed home” for EU mineral producers, EMFEMA is not only an interface between industry and institutions but also a key partner in safeguarding food chain resilience. NAVIGATING A COMPLEX LANDSCAPE The feed sector is notoriously intricate, governed by layers of scientific, legislative, and trade considerations. For many mineral producers — whose activities often span various industries such as construction, electronics, and pharmaceuticals — feed represents only a segment of their business. Within this niche, EMFEMA ensures that the voice of feed-focused mineral producers is heard, understood, and supported at EU and international levels. Minerals occupy a peculiar and sometimes ambiguous position in regulatory frameworks. They exist at the crossroads of feed materials and additives, straddling various chemical legislations. The classification, handling, and authorization of mineral substances for feed use often involve complex safety, environmental, and technical evaluations. EMFEMA’s role is to navigate this maze and advocate for proportionate, science-based regulation that secures both safety and access. STRATEGIC ENGAGEMENT FOR A SUSTAINABLE FUTURE To be effective in its mission, EMFEMA prioritizes agile and targeted engagement with policymakers, scientists, and partner organizations. It is a member of FEFAC (the European Feed Manufacturers’ Federation) and maintains close ties with regulatory bodies at the European Commission, EFSA, and national authorities. This proactive, collaborative approach is essential in addressing the pressing challenges facing the mineral feed sector. Two overarching issues define the current landscape: Preparedness in the face of geopolitical instability and securing access to the EU market. Together, these challenges shape EMFEMA’s strategic focus. PREPAREDNESS AND STRATEGIC AUTONOMY In recent years, the geopolitical context has grown increasingly unstable, with global supply chains experiencing frequent and severe disruptions. The COVID-19 pandemic, energy crises, and geopolitical tensions have exposed Europe’s vulnerability in securing key raw materials. For feed ingredients that rely on imports, often as SAFEGUARDING EUROPE'S ACCESS TO ESSENTIAL MINERALS FOR ANIMAL NUTRITION Didier Jans Secretary General EMFEMA LEAD ARTICLE

FEED & ADDITIVE MAGAZINE 7 July 2025 a result of commercial motivations, this instability poses a serious threat to the continuity of animal production. As a result, the EU is shifting its rhetoric from passive support for a “level playing field” to an active pursuit of strategic autonomy. For decades, reliance on low-cost imports undercut European producers, weakening local industries and supply resilience. EMFEMA supports the emerging recognition that autonomy — the ability to source and process critical materials within Europe — is essential to long-term sustainability and food security. This doesn’t mean rejecting international trade. Some raw materials are inherently scarce in Europe and must be imported. But it does mean reassessing our capacity to develop domestic sources. Unfortunately, opening or extending mineral extraction operations in Europe has become extremely difficult due to public opposition, environmental constraints, and regulatory burdens. The reality is that ensuring domestic access to essential minerals will require political will, societal compromise, and investment — particularly in times of global uncertainty. THE REGULATORY PARADOX: SAFETY VS. AVAILABILITY Market access is the second major concern. The EU’s feed additive regulation is one of the most comprehensive and safety-focused frameworks in the world. While this provides strong consumer and animal protections, it also presents a growing set of challenges for mineral producers. Some trace elements with decades-long records of safe use — such as selenium — are facing mounting regulatory hurdles. Environmental risk assessments, for instance, are increasingly demanding. While important in principle, these assessments sometimes extend beyond what seems proportionate to actual risk, particularly when they endanger access to basic nutritional components. This dynamic creates a paradox: Regulations designed to ensure safety are, in some cases, undermining availability. For niche minerals or substances used in non-mainstream animal categories, the cost and complexity of authorization can exceed the potential market value. Even more problematic is the perception of regulatory uncertainty. When mineral producers view the EU feed market as unwelcoming or unprofitable, they redirect products to other applications or geographies — reducing supply options for European livestock producers. A CALL FOR PROPORTIONALITY AND REFORM EMFEMA strongly believes that the EU regulatory framework needs an urgent recalibration. In particular, the authorization process for feed additives should be revised to introduce more proportionality, predictability, and socioeconomic context into decision-making. For more than five years, discussions about revising the regulation have been on the European Commission’s agenda, yet progress remains slow. EMFEMA urges regulators to recognize the growing impact of overregulation and to address the unintended consequences it creates — especially when it threatens the supply of vital nutrients for animals. Revisions must not compromise safety, but they must also reflect economic realities and ensure that authorization processes are accessible and appealing to a diverse range of producers. Without such reforms, the EU risks losing not just supply diversity, but its strategic autonomy in mineral sourcing. EMFEMA’S ROLE: A SMALL PLAYER WITH A STRATEGIC VOICE Though EMFEMA may be a relatively small player within the broader feed industry, it occupies a strategic position. By bringing together specialized mineral producers and serving as their voice in Brussels and beyond, EMFEMA is helping to ensure that Europe's livestock sector can continue to rely on a steady, safe, and sustainable supply of mineral nutrients. As the EU continues to grapple with geopolitical uncertainty, supply chain fragility, and the green transition, securing access to essential feed minerals is not just an industry concern — it is a matter of food security, economic resilience, and strategic foresight. In this mission, EMFEMA stands ready to collaborate, propose, and drive forward practical solutions that balance safety with availability, and regulation with realism.

NEWS 8 FEED & ADDITIVE MAGAZINE July 2025 A new review article, authored by researchers Kiranpreet Kaur from Aker ǪRILL Company and Dr. Silvia Torrecillas from the University of Las Palmas de Gran Canaria with support from the Institute of Agrifood Research and Technology (IRTA), explores the impact of krill-based ingredients on a range of non- salmonid aquaculture species, including sea bream, seabass, tilapia, and olive flounder. As aquaculture continues to diversify beyond salmon, this review evaluates how krill meal and krill oil can support fish health and growth even in low fishmeal and fish oil diets. With growing demand for sustainable seafood and press mounting on wild fish stocks, the aquaculture industry is increasingly seeking efficient and effective feed ingredients that balance performance, cost, and sustainability. Krill has emerged as one such tool. “Krill meal offers a unique combination of highly digestible protein, phospholipid-bound omega-3s, and functional compounds like astaxanthin and chitin,” explains Dr. Silvia Torrecillas, co-author of the review. “This makes it particularly valuable in diets for marine fish that are sensitive to changes in the feed formulation.” The review highlights strong evidence from multiple species showing that krill supplementation consistently improves feed intake, weight gain, and feed conversion ratio (FCR). In some studies, juvenile fish fed krill meal grew up to 30% faster, with FCR improvements of up to 17% compared to control groups. This effect is largely attributed to krill’s palatability and nutrient bioavailability – even at low inclusion levels. Read more>> A first-of-its-kind study led by Queen’s University Belfast reveals contaminated crops on farms could be costing poultry organisations over £150,000 in losses annually. Published recently in the journal Environmental Impact Assessment Review, the ground-breaking study shows how harmful chemicals known as mycotoxins, can have a detrimental impact on the environmental and economic sustainability of the global poultry industry due to feed contamination. As poultry production is one of the fastest-growing livestock industries worldwide due to the increase in demand for poultry meat and eggs, the presence of these chemicals in animal feed presents a significant challenge for livestock farmers across the globe. According to the research, they can substantially reduce profitability and cause food waste, as well as a decrease in food production, resulting in a reduction in poultry feed efficiency by up to 10%. In turn, this can lead to losses of more than £150,000 per annum for a medium scale poultry operation. In addition, the impacts of these mycotoxins on birds can result in poor health, even mortality. Professor Chris Elliott from the School of Biological Sciences and Institute for Global Food Security at Queen’s University Belfast is the lead investigator of the study. “While the effects of mycotoxins on livestock health have been extensively studied, comprehensive research quantifying their combined economic and environmental impacts remains very limited," said Elliott. Read more>> New review highlights benefits of krill meal in non-salmonids New Research: Mycotoxins cost poultry farms £150k Photo: Aker ǪRILL Company Photo: Queen’s University Belfast

NEWS FEED & ADDITIVE MAGAZINE July 2025 9 Agrifirm and UBM Group agreed that Agrifirm Hungary will become part of UBM Group, marking an important step in the further consolidation of the agricultural sector in Hungary. UBM Group is an agricultural group specializing in crop trading, compound feed production, and the supply of high-quality feed ingredients in Hungary. The transaction is expected to be completed in August, 2025. Piet Hilarides, CEO Royal Agrifirm Group, remarked: “Thanks to the many years of dedication and commitment of our employees Agrifirm Hungary has grown into a financially healthy and solid company. The sale of our subsidiary is in line with our strategic course, in which we focus on markets where we have a strong position and can achieve further growth. We are convinced that our Hungarian activities can perform even better with a strong partner such as UBM. We thank our members, customers, partners, and employees for their loyalty and trust, and we are committed to a careful and smooth transition.” Read more>> Agrifirm sells Hungarian subsidiary to UBM Group Polluting CO₂ is turned into nutritious feed for farmed salmon. All it takes are three innovative research environments, a visionary actor in the heavy metal industry, and large quantities of microalgae, according to a recent statement by Nofima. An emission of 300,000 tonnes of CO₂ annually at Finnfjord AS, a ferrosilicon producer located in Northern Norway, is captured in microalgae. The algae use carbon dioxide (CO₂) as an energy source. You could say the algae “eat” CO₂ – just as plants on land do. Nofima produce the feed that includes the algae: “We incorporate the algae into the feed and conduct growth trials with the salmon, from fry to slaughter weight. It is technically challenging, but results so far show that the salmon grow well on the algae feed and are in good health,” says Senior Scientist Sten Siikavuopio, who leads Nofima’s part of the project. The goal of Finnfjord AS is zero emissions – and to utilise all the CO₂ generated by the plant. The aim of turning CO₂ into food for diatoms – which in turn become valuable ingredients in salmon feed – is being pursued by UiT – The Arctic University of Norway, SINTEF, and Nofima in collaboration with Finnfjord AS. One of the major challenges in the global aquaculture industry has been sourcing enough marine oils for salmon feed. Read more>> New project converts industrial CO2 into algae-based salmon feed Photo: Courtesy of Agrifirm Photo: Finnfjord AS

NEWS 10 FEED & ADDITIVE MAGAZINE July 2025 VIV MEA, the premier international trade show for the animal husbandry and agribusiness industry in the Middle East and Africa, officially opened visitor registration for its highly anticipated 2025 edition. The event will take place from 25–27 November at the Abu Dhabi National Exhibition Centre (ADNEC) in the heart of the United Arab Emirates (UAE) capital. VIV MEA has firmly established itself as the region’s go-to platform for professionals involved from feed to food, covering all sectors of the animal protein supply chain, according to the announcement. This dynamic edition will once again bring together industry leaders, innovators, and stakeholders from around the world for three packed days of business networking, cutting-edge technology showcases, and knowledge exchange. From poultry, dairy, and fish to eggs, cattle, calves, goats, and camels, VIV MEA 2025 will explore a comprehensive spectrum of animal husbandry. With a packed conference program, attendees can expect forward-thinking sessions that cover the latest trends, challenges, and opportunities in sustainable farming, animal health, biosecurity, production efficiency, and more. The exhibition floor will feature 500+ global and regional exhibitors, offering immersive product displays and live demonstrations of state-of-the-art solutions in feed production, veterinary services, processing equipment, and agri-tech innovation. With 10,000 expected visitors onsite and a packed conference program, VIV MEA is the perfect opportunity for industry professionals to discover new suppliers, products, and partnerships—all in one place. Read more>> Evonik stated that it will continue to optimize its global methionine production setup for MetAMINO® (DL-methionine) in the third and fourth quarter of 2025. Implementation of several technical measures requires a shutdown of the production facilities for methionine and its precursors for 6-7 weeks each at Evonik's Singapore site in August/September and at its Antwerp, Belgium, site in September/October 2025. In Singapore, the maintenance schedule of a raw material supplier necessitates an early shutdown in Q3. Evonik explains it will synchronize this with the catalyst exchange in its methionine production and take this opportunity to further improve the energy efficiency and CO2 emissions of the plant. In Antwerp, Evonik will also combine a catalyst exchange with the continuation of the measures begun in May to reduce nitrogen, sulphur, and CO2 emissions. During the shutdown of the methionine facilities for 6-7 weeks in September and October, Evonik Antwerp site operations will implement further milestones from its climate roadmap and make the site’s utilities infrastructure fit for the future. These upgrades are expected to be major enablers contributing to further reductions in the carbon footprint of Evonik’s production facilities. Read more>> Registrations opened for VIV MEA 2025 Evonik to continue optimizing its global methionine production sites Photo: VIV MEA Photo: Evonik

NEWS FEED & ADDITIVE MAGAZINE July 2025 11 Cargill is investing nearly $90 million in automation and new technologies at its Fort Morgan, Colorado, USA, beef plant as part of its Factory of the Future initiative. The enhancements, which will take place over the next several years, will help Cargill continue to improve operational efficiencies, increase yields and make the Fort Morgan facility even safer and more inclusive for employees. The company has already invested nearly $24 million in technology upgrades at Fort Morgan since 2021. One of the first and most revolutionary automated solutions to be implemented at the Fort Morgan plant will be CarVe, Cargill’s proprietary, patent-pending computer vision technology. CarVe measures red meat yield in real time, giving frontline managers instant insights and the ability to share feedback with employees to improve their cutting technique. CarVe helps keep more protein in the food system that otherwise would be lost in the process, Cargill points out. According to the USDA, the U.S. produces more than 27 billion pounds of beef annually. Even a one percent yield improvement can save hundreds of millions of pounds of meat. And with the U.S. cattle supply at its lowest level in years, improving yield matters more than ever. “Before CarVe, yield data was always yesterday’s news,” said Jarrod Gillig, Senior Vice President of Cargill’s North American Beef business. “Now, we’re making decisions in the moment and saving product that would’ve been lost. By applying smart technology to the problem, we’re getting more meat from every animal, reducing waste, and making protein production more efficient and sustainable from start to finish.” Read more>> Cargill to invest $90 million in US beef plant Photo: Cargill Finnish biotech company Enifer announced that its proprietary PEKILO® fermentation technology will be deployed for the first time in Latin America in collaboration with FS, one of Brazil’s leading producers of ethanol. The project represents a significant step in the internationalization of PEKILO®Pet and PEKILO®Aqua production, marking the first time the process will operate outside Europe, as well as the first use of corn ethanol-derived thin stillage as feedstock for the production, according to the Enifer. FS is now working to construct an industrial process at a pilot scale for the Brazilian market after validating their thin stillage as a feedstock for PEKILO®Pet and PEKILO®Aqua mycoprotein production. Enifer explains that PEKILO®Pet and PEKILO®Aqua mycoproteins are nutrient-rich ingredients produced through a biomass fermentation process that utilizes a unique fungal strain, akin to the techniques employed in brewing or producing soy sauce. This process yields ingredients that are rich in protein (60%) and beta-glucan fiber, low in fat and carbohydrates, and highly palatable and digestible sources of complete protein. In pet nutrition, the micronutrients and water absorption properties of PEKILO®Pet make it an ideal ingredient that allows pet food companies to offer consumers innovative new products while reaching their own sustainability goals, adds the company. Read more>> Enifer and FS produce mycoproteins from corn ethanol side streams in Brazil Photo: ©liro Muttilainen

NEWS 12 FEED & ADDITIVE MAGAZINE July 2025 The Groan Group, an internationally operating trading company in agricultural raw materials, and Aminola®, supplier of sustainable plant-based ingredients, announced a strategic partnership. Through Groan’s financial participation in Aminola®, the two companies are joining forces to build a future-proof, plant-based food chain. Aminola® and Groan share a clear ambition: To meet the growing demand for high-quality, plantbased ingredients. Over recent years, Aminola® has established itself as a trusted partner in the food, pet food and aquafeed markets. Recognising this expertise, Groan brings in its extensive trading knowledge and a strong global network. “We believe in Aminola®’s mission and see a natural alignment with our own core values,” said Anton de Groot, owner and Managing Director of Groan. “Together, we can accelerate, expand, and strengthen sustainable supply chains – with a particular focus on the growth markets of Food and Pet Food.” Aminola states that, for the company, the partnership represents a significant boost in achieving its mission to make sustainable plant-based ingredients widely accessible. “This collaboration gives our mission a real boost! Our partnership is centred on knowledge- sharing, joint investment, and unlocking new sources,” expressed Dick van Beek, CEO of Aminola®. Read more>> Researchers from University of Connecticut’s (UConn) College of Agriculture, Health and Natural Resources (CAHNR) and College of Liberal Arts and Sciences (CLAS) demonstrated that a novel protein-based nanoparticle can make mRNA vaccines more effective to tackle a troublesome pathogen in chickens. Mazhar Khan, Professor in the Department of Pathobiology and Veterinary Science, Challa V. Kumar, Emeritus Professor in the Department of Chemistry and graduate students Anka Rao Kalluri and Aseno Sakhrie collaborated over several years and published their findings in Vaccines. Infectious Bronchitis Virus (IBV), a rapidly spreading coronavirus, is a major concern for poultry farmers in the U.S. and worldwide, as they lose millions each year due to this disease. Currently, farmers use live attenuated vaccines or killed vaccines to combat the virus. However, these kinds of vaccine come with a series of challenges. The virus could reactivate, mutate, or recombine to create a vaccine-resistant or more severe strain. These vaccines also have a shorter shelf life and require additional compounds, known as adjuvants, to be effective. The researchers have developed an effective mRNA IBV vaccine alternative. mRNA vaccines, like the human COVID-19 vaccines, do not contain any live virus. Instead, the mRNA encodes a piece of the virus’ genetic code, specifically the spike protein that is responsible for triggering the immune response and trains the immune system to respond to the protein. Read more>> Groan and Aminola join forces to meet plant-based ingredient demand New nanoparticle mRNA vaccine targets IBV in chickens Photo: Aminola® Photo: Henrique S. Ruzzon for Unsplash

NEWS FEED & ADDITIVE MAGAZINE July 2025 13 The first fishing season in Peru’s north-central region began on April 22nd, 2025, a week later than in 2024, the Marine Ingredients Organization – IFFO explained. To date, around 70% of the 3-million-mt anchovy quota has been fulfilled. These materials are key to fishmeal and fish oil production, as Peru contributes 20% on average to global production. “IFFO’s 2025 estimates for fishmeal and fish oil are at 5.6 million tons of fishmeal and 1.2-1.3 million tons of fish oil,” Dr. Enrico Bachis, IFFO’s Market Research Director, has commented. In April 2025, according to IFFO’s market intelligence reports* the total cumulative fishmeal production increased by approximately 3% compared to the same period in 2024. This increase was driven by growth in all areas except for the North European countries, the only area to report a negative performance compared to January–April 2024. As for fish oil, total cumulative output for the year through April 2025 was 14% up year on year. Similarly to fishmeal, almost all the countries reported a positive trend in cumulative fish oil production compared to January-April 2024, with the exception of the European countries. China’s domestic production of marine ingredients in the first five months of 2025 was estimated to be lower than during the same period a year ago. The fishing bans are now in place with most local fishmeal plants now idle. The main raw material for producing fishmeal and fish oil for the time being will come from frozen fish, imported sardines and by-products from processing facilities. Read more>> Global fishmeal production rises despite regional declines Photo: IFFO Gut health is more than a gut instinct. Improving bird productivity through clean feed. Get the study today. www.anitox.com/broiler-performance

NEWS 14 FEED & ADDITIVE MAGAZINE July 2025 One of the global leaders in animal nutrition, Alltech increased its stake and acquired Finnforel’s share of Alltech Fennoaqua Oy, increasing its shareholding and becoming the company’s majority stakeholder. Alltech points to this milestone as reinforcing its commitment to the Finnish aquaculture sector and to advancing sustainable fish farming across northern Europe. Alltech Fennoaqua is Finland’s only fish feed production facility and a pioneer in nutrition for recirculating aquaculture systems (RAS). It also specializes in producing sustainable, high-quality feed solutions tailored for cold-water aquaculture operations. Alltech delivers tailored nutritional solutions that improve health and performance while reducing impact on the environment. The company highlights its increased investment in Alltech Fennoaqua as unlocking new opportunities for innovation and growth in the aquaculture sector — both in Finland and worldwide. “Aquaculture is one of the world’s fastest-growing sources of protein – and it is a focus area for us,” said Dr. Mark Lyons, president and CEO of Alltech. “As majority owner of Alltech Fennoaqua, we are deepening our commitment to delivering smarter, more sustainable solutions for aquaculture systems, backed by scientific innovation and global aquaculture expertise.” Alltech and Finnforel first partnered in early 2023 to acquire the Raisioaqua fish feed production facility, safeguarding Finland’s only source of domestic fish feed. Since then, the company, renamed Alltech Fennoaqua, has enhanced production to meet growing demand for RAS and cold-water aquaculture feeds. Read more>> Phileo by Lesaffre, a global leader in fermentation and microbial solutions, announced that the European Food Safety Authority (EFSA) published a scientific opinion on its innovative bacteria probiotic, Microsaf®, which concludes on its efficacy for chickens and other poultry for fattening as well as on its safety for the target species, consumers and the environment. Microsaf® is a unique association of 3 Bacillus strains, powered with GO Technology, an innovative technology allowing Bacillus spores to germinate and deliver benefits more efficiently. The product has shown positive effects on digestive health and performance of broiler chickens, according to the announcement. The EFSA opinion confirms Microsaf®'s potential as a zootechnical additive in improving performance for poultry for fattening. Phileo by Lesaffre sees this positive scientific assessment as a crucial milestone in Microsaf®'s EU journey for the authorization as zootechnical feed additive, in the functional groups ‘gut flora stabilisers’ and ‘other zootechnical additives’. "We are thrilled to receive this positive EFSA opinion, which validates the efficacy and safety of Microsaf®," said Elen Rondel, Western Europe Poultry Manager at Phileo by Lesaffre. "Microsaf® is a testament to our commitment to providing reliable, science-backed solutions that support animal health, performance and welfare." Read more>> Alltech becomes majority owner of Alltech Fennoaqua EFSA publishes positive scientific opinion on Phileo by Lesaffre’s bacteria probiotic Photo: Freepik Photo: Alltech

NEWS FEED & ADDITIVE MAGAZINE July 2025 15 The Future of Animal Nutrition Starts Here © 2025, Cargill Incorporated. All rights reserved. Experience our new brand portfolio of science-based solutions, developed with our passion and commitment to move animal agriculture forward. Come see what the new era of micronutrition and health solutions brings. Micronutrition & Health Solutions Proven animal performance. With purpose.

NEWS 16 FEED & ADDITIVE MAGAZINE July 2025 With the theme “Multiplying impact together”, the Networking Days 2025 of Bühler highlighted the courage to navigate uncertainty and the solutions available now to build successful companies that bring positive impact at massive scale. Over 1,200 business leaders from the food, feed, and sustainable mobility and materials sectors gathered at the company’s headquarters in Uzwil, Switzerland, from June 23rd to 24th, address the urgent challenge of building successful businesses that feed and move 10 billion people sustainably by 2050. Many key solutions are in place that meet these challenges. What is needed now is to multiply their impact at scale. Speaking at the event, Bühler Group CEO Stefan Scheiber described the power of collaboration and cooperation to multiply the impact of innovation. “Every breakthrough, partnership, and bold decision has the potential to create ripples – spreading knowledge, inspiring action, and driving progress,” he said. “But their true power lies in the multiplier effect: when these ripples connect, they create waves of change. By working together, businesses and industries don’t just add incrementally to progress – they accelerate it by compounding their influence and scaling solutions far beyond what any single effort could achieve.” Ian Roberts, Bühler Group CTO expressed: “It is so clear now that we must act with focus and collaboration to bring the impact necessary to preserve the healthy state of our planet. I am energized by the potential and willingness shown by our 1,200 guests – not to simply talk, but to build concrete actions and to share what they have already achieved to accelerate group learning and impact multiplication.” The Networking Days 2025 was the fourth Bühler Group Networking Days event. The Swiss-based technology group has convened leaders from the industries it serves once every three years since 2016. Attendees at this year's event traveled from 90 countries and six continents. Read more>> dsm-firmenich Animal Nutrition & Health announced the launch of GutServ® Biotix, an innovative postbiotic solution designed to support piglet health, resilience and performance. Maintaining piglet health and performance is one of the biggest challenges in modern pig production, especially during critical stress periods such as weaning. Strengthening gut integrity and functionality, dsm-firmenich highlights GutServ® Biotix as helping minimize early-life setbacks and allowing piglets to thrive efficiently and establish a solid foundation for healthy growth and sustained performance. Backed by robust scientific validation and multiple trials across geographies, GutServ® Biotix empowers producers to build stronger pigs from the gut up—confidently and consistently, with a proven return on investment ranging from 5:1 to 10:1, it offers consistent value across diverse production systems, according to the announcement. GutServ® Biotix's compatibility with all feed types and processing conditions makes it a flexible solution for modern operations. The formulation is heat-tolerant, stable, and easy to handle, ensuring seamless integration into existing workflows. Read more>> Bühler Networking Days 2025 highlights scalable solutions for sustainablility dsm-firmenich introduces postbiotic additive for piglets Photo: Courtesy of Bühler Photo: dsm-firmenich Photo: ChainCraft

NEWS FEED & ADDITIVE MAGAZINE July 2025 17 BioCraft Pet Nutrition™, a biotech company that develops animal cell-cultured ingredients for the pet food market, released the results of a new product carbon footprint analysis, conducted in collaboration with ClimatePartner, a solution provider supporting companies on their journey towards net zero. The analysis showed that its proprietary BioCrafted Meat™ emits one-twelfth of the carbon dioxide of conventional beef byproducts used in pet food. According to the analysis, based on standard EU beef production processes, a hypothetical beef byproduct emits 21.28 kg CO₂ per kilogram—in comparison, BioCrafted Meat produces just 1.73 kg CO₂ per kilogram. The calculation was based on internationally recognized methodologies, including the Greenhouse Gas Protocol Corporate Accounting and Reporting Standard (GHG Protocol) and the Product Life Cycle Accounting and Reporting Standard (PDF), using emission factors from trusted databases such as ecoinvent, Agri-footprint, and DEFRA. According to the company's statement, the comparison to byproducts not typically consumed by humans—like offal, bones, blood, and fat—rather than prime beef cuts is significant because the pet food industry, and consumers, often regard these byproducts as sustainable, low-impact alternatives. Read more>> New Analysis: BioCraft ingredient emits 1/12 less CO2 than beef byproducts Photo: BioCraft Supplant D The natural antioxidant • Improves intestinal health • Enhances animal performance • Vitamin E sparing effect • Protects against all kinds of stress agrimprove.com Vitanox

NEWS 18 FEED & ADDITIVE MAGAZINE July 2025 Trouw Nutrition unveiled poultry nutrition research findings during the European Symposium on Poultry Nutrition (ESPN), in Maastricht, the Netherlands, from 23-26 June, 2025. During the biennial scientific gathering organised by the Netherlands and Belgium chapters of the World Poultry Science Association, the company hosted two oral presentations and nine scientific posters, with research spanning broiler and layer production systems. The company shared the following research at the event: Ongoing research at the Nutreco Garden of the Future in Switzerland is expanding the development of novel phytoComplex solutions as dietary tools to improve poultry health and performance, along with producers’ businesses. Findings from several broiler studies and layer programmes involving research conducted on product development from the Nutreco Garden of the Future were shared at ESPN. The company points out that: • Two products were tested as potential immune support for broilers given a Salmonella typhimurium vaccine. Results for broilers receiving the PhytoComplex (Fytera Care) or phytogenic (a competitor) supplements suggested that adding specific natural plant extracts at precise levels could make Salmonella vaccines more effective without harming bird performance. • A series of studies explored the influence of a novel blend of phytoComplex materials mixed with target-release butyrates for high-performing broilers. Analysing data from all trials found that including the feed additive improved body weight gain and tended to improve feed efficiency in high-performing broilers compared to control results. • Research also looked at the early translocation of enterococcus cecorum from the bird’s gastrointestinal tract into other organs. Enterococcus cecorum is the leading cause of kinky back disease, which impairs broiler mobility and growth performance. Research findings showed that E. cecorum translocates very early in broilers, which can be influenced by nutritional strategies, including the use of phytoComplex blends for early intervention and disease management. Read more>> ADM, one of the global leaders in innovative solutions from nature, opened a new research and development (R&D) center focused on advancing research into animal microbiomes, and developing tailored products within the health and wellness (H&W) industry for pets, livestock and aquaculture. In the 1,600-square-meter facility, located at the Biopôle life science campus on the north side of Lausanne, Switzerland, ADM’s scientists will undertake innovative pre-clinical research to tackle health challenges using biotics and other fermentation-derived ingredients that support the health and well-being of pets and farm animals. "We are seeing significant growth in the global pet and farm animal microbiome market, as farmers and pet owners alike seek science-backed nutrition solutions to support the health and well-being of their pets and livestock. We are proud to launch this stateof-the-art research and development center, uniquely dedicated to microbiome research for pet and farm animal health and well-being, with the flexibility to adapt to emerging research demands," said Dr. Nuria Miquel, Senior Vice President and Chief Science Officer at ADM. "The center will deploy cutting-edge sciTrouw Nutrition shares poultry production insights at ESPN 2025 ADM opens microbiome research hub for pet and livestock health Photo: Trouw Nutrition

NEWS FEED & ADDITIVE MAGAZINE July 2025 19 entific and technological tools to increase our understanding of the role of the gut microbiome in broader pet and animal health, building on our existing work in both human and animal nutrition, and support our development of science-backed microbiome solutions to bring to market for our customers. It represents a significant step forward in our purpose to advance animal wellness through science and innovation." The global biotics in animal feed market had an estimated value of $5.2 billion in 2024. The gut microbiome has been linked to immune, digestive and absorptive functions, thereby affecting animal protein production and resource usage across species. Producers looking to optimize efficiency in performance and profitability are increasingly incorporating functional feed additives into animal diets. Microbiome-supporting solutions are also important to pet owners who want to keep their companions healthy and active as long as possible. Notably, 84% of pet owners surveyed in 22 countries say they are interested in products that may help increase the lifespan of their pet. With this growing interest in functional ingredients for companion animals, ADM R&D leverages its global network to conduct studies on the effects of pre-, pro- and postbiotics for pet well-being. For example, a recent clinical trial demonstrates postbiotic benefits for metabolic health in adult dogs by supporting healthy blood sugar levels, a biotic blend maintained the intestinal health of adult cats before and after a diet change, a postbiotic supports healthy teeth and gums in adult dogs and a combination of postbiotics can help maintain skin health in pets. Read more>> Swiss technology group Bühler opened a new, stateof-the-art Milling Academy – a 1,800-square-meter training center for food and feed millers – at the heart of its headquarters in Switzerland. Designed to meet the evolving needs of the industry, the facility combines decades of expertise with the latest technology and modern teaching methods. Bühler points out that direct access to research and training centers enables course participants to gain unique hands-on insight into key industry areas. The milling industry plays a vital role in global food security, but it is also navigating an era of significant change and challenge. In addition to ever-higher food safety standards and the need for efficiency and sustainability, millers are facing increasing pressures from climate change, a shrinking skilled workforce, and supply chain volatility. Against this backdrop, technology and digital solutions are emerging as powerful tools to boost yield, profitability, and resilience. However, realizing the full potential of these advancements depends on a well-trained workforce. This makes advanced, hands-on training programs for millers more important than ever before. “With the opening of our new, state-of-the-art Milling Academy, we are empowering the next generation of milling professionals by offering advanced training, hands-on experience, and access to the latest technologies,” said Stefan Birrer, Head of Business Area Milling Solutions at Bühler Group. “This ensures customers can meet evolving industry demands and drive continuous improvement throughout the food and feed sectors.” Read more>> Bühler opens Milling Academy for food and feed milling professionals Photo: Courtesy of Bühler Photo: Freepik

NEWS 20 FEED & ADDITIVE MAGAZINE July 2025 Although bird flu, or H5N1 influenza, had been primarily limited to wild migratory birds and sporadic outbreaks in commercial poultry operations, it was detected in U.S. dairy cows in March 2024. Since then, H5N1 has spread rapidly in dairy cattle across multiple states, and the industry has limited tools to protect their herds. Together, the Foundation for Food & Agriculture Research and Purdue University are investing $301,562 into a Rapid Outcomes from Agricultural Research (ROAR) grant to develop an H5N1 vaccine for dairy cows. While H5N1 does not have high mortality rates in dairy cows, the infection causes decreases milk production and milk quality, causing significant economic losses for farmers. The recent detection of H5N1 in humans, dairy cows and non-traditional host birds indicates that this virus poses new threats to other non-avian species. “The detection of avian influenza in dairy cattle, with risk of infection to dairy workers, underscores the need to develop effective vaccines for non-avian species. This rapid funding is providing the U.S. dairy industry with urgently needed tools to protect animal health, farmers’ livelihoods and consumers’ wallets,” says Miriam Martin, Ph.D., Scientific Program Manager. Read more>> The molecules that form the foundation of life on Earth are as diverse as they are complex. Among these, carbohydrates play a vital role as energy sources and in structural functions, such as forming cell walls. One class of carbohydrates, β-1,2-glucans, consists of glucose chains and is found in bacteria. These molecules are involved in various important biological processes, such as bacterial infection and environmental adaptation. Despite their biological significance, β-1,2-glucans are rare, compared to cellulose and laminarin, and structurally complex, making them particularly difficult to study. In a recent study published in Volume 34, Issue 6 of the journal Protein Science on May 24, 2025, researchers from Tokyo University of Science (TUS) made significant progress by identifying and characterizing new enzymes that break down glycan molecules. The team investigated a group of unclassified glycoside hydrolases (GH) related to known β-1,2-glucan-degrading enzymes in families GH144 and GH162. Through a combination of sequence, biochemical, structural, and phylogenetic analyzes, the team identified new phylogenetic groups that showed enzymatic activity toward β-1,2-glucans. β-1,2-glucanase (SGL) breaks down β-1,2-glucan into β-1,2-glucooligosaccharides. The study was conducted by a team at TUS, led by Associate Professor Masahiro Nakajima and supported by former doctoral student Dr. Sei Motouchi, with additional collaboration from Associate Professor Hiroyuki Nakai of Niigata University and Dr. Kaito Kobayashi of National Institute of Advanced Industrial Science and Technology. Read more>> $301K grant funds H5N1 vaccine for dairy herds Scientists discover new enzyme families that break down rare bacterial carbohydrates Photo: FFAR

NEWS FEED & ADDITIVE MAGAZINE July 2025 21 Farmina Pet Foods, one of the global leaders in premium pet nutrition, officially opened its first U.S.-based manufacturing facility in Reidsville, North Carolina, marking a major milestone in the company's 50-year history. While new to U.S. manufacturing, Farmina has served North American pet owners for more than a decade, delivering premium pet food made with European ingredients, upheld by rigorous standards and cutting-edge technology. The 150,000-square-foot stateof-the-art manufacturing facility, located at 248 Sands Road, Reidsville, represents a $115 million investment and is expected to create 200 new jobs over the next five years, with 75 positions already filled. New roles include machine operators, veterinarians, chemical technicians, shipping clerks, maintenance technicians, managers, and supervisors. To celebrate Farmina's investment in the local and state economies, North Carolina State Senator Phil Berger, Assistant Commissioner for Consumer Protection, NC Department of Agriculture Chrissy Waggett, Chairman of the Rockingham County board of Commissioners Kevin Berger, and Mayor of Reidsville Donald Gorham, attended the official opening of the facility on June 11, 2025. Senator Phil Berger welcomed the expansion, stating, "Farmina's decision to establish its U.S. operations in North Carolina is a strong endorsement of our skilled workforce and our growing reputation as a business-friendly hub for modern, innovative manufacturing." He continued, "We're thrilled to welcome this world-class technology to our great state and proudly open our arms to Farmina as they join our business community." Read more>> Italian pet food company expands operations to US with new facility Photo: Farmina A front-runner in the production of sustainable chemicals of food waste, ChainCraft entered a long-term exclusive supply agreement for butyric acid to the animal nutrition sector with Innovad Group, one of the global leaders in animal health and nutrition solutions. Through this collaboration, ChainCraft will supply fatty acids of high purity to Innovad, enabling the production of low carbon advanced feed additives that support both animal intestinal health and environmental sustainability, according to the companies’ statement. This partnership not only promotes sustainability within the animal feed industry but also brings a unique synergy between two innovators. ChainCraft’s cutting-edge fermentation technology transforms organic residues into high-value short & medium chain fatty acids, providing a sustainable alternative to traditional fossil and palm oil-based products. Innovad, headquartered in Belgium, specializes in natural, science-backed feed solutions that improve animal welfare and performance, while minimizing environmental impact. Innovad points to its portfolio as supporting farmers globally in lowering disease occurrence, mortality and feed requirement - all of which improves animal welfare and sustainability of their farm. By integrating ChainCraft’s bio-based fatty acids into its product portfolio, Innovad aims to enhance the health and nutrition of livestock while reducing dependency on non-renewable resources. Read more>> ChainCraft and Innovad partner for sustainable feed Photo: ChainCraft

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