FreezeM has publicized its new method for black soldier fly (BSF) larvae sex identification using Polymerase Chain Reaction (PCR). Through the method, the larvae reportedly continue developing into adults capable of reproduction, making it practical and sustainable for ongoing breeding programs.
One of the leaders in black soldier fly (BSF) breeding, FreezeM’s R&D team has developed a groundbreaking method for identifying male and female BSF larvae using Polymerase Chain Reaction (PCR), revolutionizing breeding and rearing practices. Previously, sex differentiation was only possible in adults through a manual, one-by-one process under a microscope, according to the company. Early identification during the larval stage, especially in a high-throughput manner, presents significant opportunities to optimize breeding, rearing, and production efficiency by enabling targeted management of each sex.
The company explains the key findings as:
● Innovative Sex Differentiation: Our R&D team discovered a unique genetic sequence distinguishing male and female BSF. By amplifying this sequence with PCR, we can now accurately determine the sex of larvae.
● Non-Invasive and Sustainable: This non-destructive method allows larvae to be tested through a harmless biopsy. The larvae continue developing into adults capable of reproduction, making it practical and sustainable for ongoing breeding programs.
● Impact on Breeding and Rearing: Early sex differentiation enables more precise breeding and rearing strategies. Producers can optimize male/female population balances for specific objectives, improving resource management, product consistency, and economic outcomes.
● High-Throughput Application: This PCR-based approach supports large-scale operations by enabling high-throughput screening. Early sex identification streamlines selective breeding programs and promotes the development of genetically superior lines for targeted traits.
The development of this PCR method marks a significant milestone in BSF research, paving the way for enhanced efficiency and sustainability in insect-based protein production. This breakthrough gives producers a competitive edge by optimizing operations and meeting specific market demands.
