ISSUE FOCUS 42 FEED & ADDITIVE MAGAZINE December 2025 * * * * * * 19 1 20 20 Spontaneous Acceptance 1st Choice 91.5 82.1 8.5 17.9 94.7 82.6 5.3 17.4 94.6 87.8 5.4 12.2 Day 1 Day 2 Day 3 20 20 Day 1 Day 2 Day 3 20 16 12 8 4 0 20 Results from Trial 1 (Diet A & Diet B: Premium dog food + Chicken oil (3%) + Liquid premium digest (3%)) *...P<0.05 (ANOVA, post-hoc Tukey HSD test) D: Premium dog food + Chicken oil (3%) + Liquid premium digest (3%) + PetArom Beef P (20 g / 100 kg) A: Premium dog food + Chicken oil (3%) 100 80 60 40 20 0 Diet Consumption (%per day) Number of Dogs (out of 20) sensory appeal of the diet and reinforces the intake-driving effect of digest. Conclusion PetArom amplifies digest performance, delivering ~10 percentage points of additional synergy (Figure 3). TRIAL 3 - PetArom Enhances Digest-Based Diets After confirming that digest serves as a key driver of palatability, PetArom was evaluated on top of a standard digest-based diet (Diet B), reflecting a typical commercial formulation. Diets offered: • Diet B: Premium dog food with 3% digest • Diet D: same diet + 20 g PetArom Beef P per 100 kg feed When PetArom was incorporated into the digest-containing diet, dogs showed a clear preference for the enhanced formulation, shifting selection to approximately 66/34 in favour of Diet D. This demonstrates a meaningful sensory uplift even in a premium digest-based matrix, confirming true synergy rather than redundancy. Conclusion PetArom significantly enhances the palatability of digest-based diets, elevating sensory appeal without requiring formulation changes. This provides a valuable differentiation opportunity for pet food manufacturers — enabling premium positioning and a more engaging eating experience through a simple, low-dose enhancement (Figure 4). TRIAL 4 – Dose Response: Confirming The Optimal Inclusion Level To evaluate whether a higher inclusion level of PetArom Beef P could further enhance palatability, an additional comparison was conducted within the same trial: • Diet B: Premium dog food with 3% digest • Diet E: same diet + 40 g PetArom Beef P per 100 kg feed Figure 3. Effects of combining PetArom Beef P with digest, demonstrating a synergistic palatability boost beyond the effect of digest alone
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