ARTICLE 70 FEED & ADDITIVE MAGAZINE November 2025 TRIPLE-STAGE SUPPORTIVE ACTIVITIES Laboratory studies indicate that Coxield™ supports coccidiosis management through three complementary biological activities: 1. Direct sporozoite viability reduction Coxield’s natural compounds weakened parasite viability, inducing necrosis and structural damage at concentrations as low as 10 ppm. 2. Limiting attachment and penetration In cell culture models, Coxield reduced parasite adhesion and penetration into intestinal epithelial cells by approximately 60% when evaluated at 7 ppm. 3. Restricting intracellular development Coxield treatment curtailed intracellular asexual development, specifically reducing merozoite formation from invading sporozoites by about 66% at 7 ppm. These three supportive activities work in concert, providing broad coverage across critical stages of the coccidial cycle. Rather than acting like a single-target chemical, Coxield™ offers a multifaceted, natural solution that helps reduce lesion severity and oocyst shedding while promoting intestinal health (Figure 4). 4. VALIDATED IN SCIENCE, PROVEN IN FARMS Academic research confirmed Coxield™’s role in coccidiosis management. Challenge trials with virulent E. tenella strains showed lower lesion scores and reduced oocyst shedding in supplemented groups compared with standard controls such as salinomycin. Product optimization using RSM further confirmed that the final formulation offered consistent performance. In vivo trials demonstrated reduced lesion scores (1.87 vs. 2.7 for salinomycin and 3.3 for challenge controls) and a 27% reduction in oocyst shedding. Farm validation provided further evidence: • In Korea, broiler farms experiencing productivity decline due to reduced diclazuril efficacy—linked to resistance—incorporated Coxield™ into feed programs. Over five flocks, the average Production Index improved from 343 to 380 after 32 days of supplementation, with clear signs of healthier intestinal conditions. This suggests Coxield™ can provide supportive benefits where conventional products face resistance challenges— functioning as a complementary solution rather than a direct replacement. • In Indonesia, high-challenge trials with four Eimeria species showed that Coxield™ performed on par with synthetic drugs under conditions where resistance had reduced the efficacy of diclazuril, while also outperforming other natural products. Importantly, when Coxield™ was combined with diclazuril, the program delivered the best outcome, improving Figure 4. Triple-stage supportive mechanism of Coxield™ Excystation 1 1 2 3 2 0 4 3 Directly kills sporozoite Inhibits cell invasion Suppresses merozoites proliferation Swallowed oocyst Oocyst shed in feces Invades gut cells Eimeria Life Cycle Inhibits the Eimeria by reducing intracellular merozoite proliferation Inhibits infection by reducing adhesion of sporozoites to the cell mebrane Suppresses the Eimeria by directly killing sporozoites released from the oocyst Triple Mode-of-Actions in the Eimeria Life Cycle Merozoites proliferation
RkJQdWJsaXNoZXIy MTUxNjkxNQ==