ARTICLE FEED & ADDITIVE MAGAZINE November 2025 103 For fat oxidation follow-up we systematically analyzed: • Peroxide Value (PV) using AOCS method which measures the primary compounds of the auto-oxidation reaction, PV target <10 meqO2/Kg of fat till the end of shelf-life, • Hexanal, as a marker for secondary compounds of fat oxidation deriving from different linoleic acid pathways, hexanal target <10ppm till the end of shelf-life, • The recoveries of antioxidant molecules like Tocopherols and Carnosic acid from rosemary extract. During the development of antioxidant blends, each molecule was tested individually and in combination to optimize the ideal ratio. The evaluation focused on identifying any lack of synergistic effects or potential pro-oxidative interactions, while minimizing cost-in-use. Another part of the research was dedicated to enhancing the activity of antioxidant molecules in the different phases such as the emulsion and final meal. In the last part of the pilot evaluation, we studied the impact of the overstress during the different rendering pilot phases such as the cooking phase and the press effect. It supported the final formulation and especially the antioxidant molecule balance in the Verafyt™ LR-35 Liquid formula. Industrial Trials’ Validation To confirm the pilot trials’ results shown in Figure 2, we scheduled industrial trials using a discontinuous dry rendering process. First, the process was cleaned with Verafyt LR-35 Liquid to avoid synthetic molecules cross-contamination RAW MATERIALS COOKING DRYING SEPARATION COOLING SIEVING / BAGGING / STORAGE Reception Bin Metal Detection Crusher Cooker Fat Drainer Decanter Fat Tank Storage Grinder Sieving Mixer Big Gag Station Meal Storage Meal Zone Cooging Zone Raw Materials Zone Fat Press 1 Press 2 AOX 1 AOX 3 AOX Figure 2. Antioxidant application points in batch dry rendering process. AOX 1 application in poultry crude raw materials and AOX 2 application in PAP after sieving.6 Photo: Freepik
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