TECHNOLOGY FEED & ADDITIVE MAGAZINE September 2025 63 highly realistic digestive journey that better reflect pig and poultry digestion physiology. The current SDS III platform includes eight standardized protocols validated against in vivo trials: four for swine and poultry feed energy evaluation and four for protein digestibility. Additional protocols are also available for amino acids, phosphorus, and carbohydrates (Image 2). VALIDATION WITH POULTRY TRIALS The crucial test for any in vitro system is whether its results align with in vivo trials. In a duck study with 30 corn samples, SDS III’s in vitro digestible energy showed strong correlation (R²=0.8842, RSD=45 kcal/kg) with true metabolizable energy. This correlation demonstrates SDS III’s reliability and its potential to replace many live-animal trials in feed evaluation (Figure 1). Image 2. The SDS III software interface, showing eight protocols validated through in vivo swine and poultry trials V5 V7 Water bath incubator Thermally stable chamber Cooling chamber V10 V11 V8 V9 V3 V4 P2 P1 P3 Digestive fluid Buffer solution P4 Wash liquid Distilled water Small intestinal buffer solution Large intestinal buffer solution Waste liquid V6 3100 3200 3300 3400 3500 3600 3700 3800 3900 IVDE, kcal/kg 4200 4100 4000 3900 3800 3700 3600 3500 3400 3300 3200 TME= 547+0.917 x EHGE (R2=0.8842, RSD=45kcal/kg) TME, kcal/kg Figure 1. The strong correlation between In Vitro Digestion Energy (IVDE) measured by SDS III and in vivo True Metabolizable Energy (TME) in ducks across 30 different corn samples, highlighting the method’s reliability and its potential to transform feed formulation and enzyme development
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