TECHNOLOGY 62 FEED & ADDITIVE MAGAZINE September 2025 • Partial gut simulations, which focus on only one section of the digestive tract. These weaknesses mean that conventional in vitro methods rarely correlate strongly with in vivo outcomes. For feed companies aiming for precision nutrition, this creates frustration and forces them back to costly live-animal validation. HOW SDS III OVERCOMES THESE BARRIERS The SDS III platform addresses the above challenges through several innovations. Absorption Simulation SDS III’s horizontal digestion module uses dialysis bags to mimic nutrient absorption, allowing amino acids, peptides, sugars, and oligosaccharides to diffuse out. This prevents feedback inhibition of enzymes and yields results that align closely with in vivo trials. A vertical module enables real-time sampling for added flexibility (Image 1). Full Gut Simulation Unlike segmented models, SDS III automates the full gastrointestinal process: stomach, small intestine, and large intestine. Controlled pH shifts, enzyme dosing, and buffer exchanges are managed by a micro-peristaltic pump, while a thermostatic system maintains temperature within ±0.4°C of animal body temperature. Together with orbital shaking to simulate chyme mixing, these precise controls allow Simulated Digestion System (SDS III) for Monogastric Animals Automated simulation of the digestive tract: -Stomach -Small intestine -Large intestine Stomach 3 h SGF enzyme Sample In In In Out Out Out Buffer Buffer 1 Buffer 2 Buffer 3 Buffer Dialysis tubing Digestive tube SSIF enzyme SLIF enzyme Small intestine 5 h Large intestine 21 h Vertical Digestion Module Horizontal Digestion Module Image 1. The SDS III platform, including its vertical and horizontal modules, and how they simulate the entire gastrointestinal tract Photo: Manop Boonpeng | ShutterStock
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