ISSUE FOCUS 32 FEED & ADDITIVE MAGAZINE September 2025 Degradation of phytogenic compounds was quantified using HPLC-UV/Vis, allowing precise monitoring of compound integrity across conditions. Multifactorial Stress – Stability in Application is Key Key findings (Figure 2) include: • Temperature was the dominant factor influencing degradation. All active substances declined significantly with rising heat. EGCG showed to be sensitive towards temperature catalyzed oxidation, with concentrations dropping by ~50% at pH 7. • pH had a complex effect. At room temperature, most compounds were more stable at pH 7 – except EGCG. Under thermal stress, EGCG, and oleuropein showed higher stability at pH 5, whereas curcumin remained consistently more stable at pH 7. • Light exposure showed negligible impact under the conditions tested. Pasteurization Sterilization Room temperature 100.0 90.0 80.0 70.0 60.0 50.0 40.0 30.0 20.0 10.0 0.0 90.2 92.2 98.5 99.2 91.1 90.6 96.3 97.2 91.8 90.6 92.4 94.5 Recovery of Oleuropein in Percent Recovery of Curcumin in Percent Pasteurization Room temperature 100.0 90.0 80.0 70.0 60.0 50.0 40.0 30.0 20.0 10.0 0.0 83.2 92.4 100.0 100.0 70.1 79.7 85.1 83.4 73.4 69.6 79.5 77.8 Sterilization pH5+ light pH5 pH7 pH7 + light Figure 2. Degradation results of phytogenic components under varying temperature, pH, and light conditions Recovery of EGCG in Percent 96.7 100.0 90.0 80.0 70.0 60.0 50.0 40.0 30.0 20.0 10.0 0.0 99.0 87.5 93.7 96.4 97.2 76.8 72.7 93.1 89.1 47.3 Sterilization Pasteurization Room temperature 48.5
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