ISSUE FOCUS 56 FEED & ADDITIVE MAGAZINE July 2025 This imbalance is called oxidative stress and leads, among other causes, to an increase in cell breakdown. In certain in vitro tests, Olive Bioactives have demonstrated their ability to reduce oxidative stress and increase the expression of Catalase, even in challenging conditions. (Figure 2) Cellular permeability On the other hand, in vitro studies conducted on an intestinal cell model (IPEC-J2) have demonstrated the positive effects of Olive Bioactives in increasing the relative expression of tight junction proteins such as occludin and claudins, even under an inflammatory challenge (LPS). This may help the intestinal barrier to properly act as a selective barrier, allowing efficient nutrient absorption while harmful pathogens are kept away. (Figure 3) Intestinal integrity From a histological perspective, crypt depth has been directly related to intestinal cell renewal, with damaged intestines showing increasing crypt depth as a sign of increased cell turnover due to the death of damaged cells. A lower depth in crypts is associated with better nutrient absorption. Catalasa Relative Expression ROS Inhibition 2.0 1.5 1.0 0.5 0.0 150 100 50 0 CON LPS OE c c d Vit E Acetate + TBHP OE + TBHP Quercetin + TBHP TBHP CON a a a b b bc OE+LPS Control Relative Expression Relative Expression LPS Claudin Occludin b b b a a 1.5 1.0 0.5 0.0 1.5 1.0 0.5 0.0 b a a OE OE+LPS Control LPS OE OE+LPS Figure 2 Figure 3 LPS: Lipopolysaccharides OE: Olive Bioactives CON: Control TBHP: Tert -Butyl hydroperoxide OE: Olive Bioactives LPS: Lipopolysaccharides.
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