ARTICLE FEED & ADDITIVE MAGAZINE February 2025 55 fed to satiation twice per day, over 4 weeks. The water temperature was maintained at 17.69 ± 0.58°C to simulate cold stress conditions. Other parameters included a pH of 7.1 ± 0.32 and dissolved oxygen at 9.05 ± 0.13 mg/L (mean ± standard error). Plant-based feed formulations are less palatable than fishmeal-based feeds, while cold stress further impairs digestive efficiency, reducing feed intake and the ability to emulsify, digest and absorb nutrients. These combined factors can negatively impact fish growth and metabolic health, and fat can accumulate in excess in the liver and viscera due to inefficient lipid utilization. The supplementation of 0.025% AQUALYSO in juvenile tilapia feed resulted in numerical improvements of 10% and 12% in feed intake and weight gain, respectively (Figure2A and 2B). Furthermore, fat deposition in the liver and viscera was reduced by 2% and 3.5%, respectively (Figure 2C), Such effect can help in alleviating metabolic issues associated with low-temperature conditions and excessive lipid accumulation in viscera. AQUALYSO enhanced fat emulsification and improved nutrient absorption across the intestinal epithelium, allowing fish to extract more energy and nutrients from the feed. This not only supported better growth performance but also reduced fat deposition in the liver and viscera, alleviating common metabolic issues associated with low-temperature conditions. By optimizing feed utilization under these challenging conditions, AQUALYSO demonstrated its ability to maintain fish metabolic health, support energy allocation for growth and mitigate the negative effects of cold stress. CONCLUSIONS Results from these trials further corroborate efficacy of the LPL-based Control Ingredients (g/kg) 0.025% AQUALYSO Soya meal Corn Wheat bran Poultry meal Rapeseed oil Fish oil Vitamins/Minerals Micronutrients Aqualyso Moisture Crude protein Crude Fat Ash 470 330 90 40 20 7 10 33 0 70.4 288.0 63.4 69.5 470 330 90 40 20 7 10 33 0.25 70.4 289.9 61.8 69.5 Table 2. Ingredients and proximate composition of the experimental feeds of tilapia (dry matter). Weight gain (g) Feed intake (%ABW/g) Difference to Control (%) 0,60 0,58 0,56 0,54 0,52 0,50 0,48 0,46 0,44 5,00 4,50 4,00 3,50 3,00 2,50 2,00 1,50 1,00 0,50 0,00 0,0 -0,5 -1,0 -1,5 -2,0 -2,5 -3,0 -3,5 -4,0 C A 10% 12% B Control 0.025% AQUALYSO Control HSI VSI 0.025% AQUALYSO Figure 2. Trial 2: Comparing a plantbased feed without (Control) and with 0.025% AQUALYSO supplementation. A: Feed intake (%Average Body Weight/g), B: body weight gain (g), and C: somatic indices (HSI: Hepatosomatic Index; VSI: Visceral Somatic Index) (mean ± standard error).
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