ARTICLE 54 FEED & ADDITIVE MAGAZINE February 2025 Largemouth bass juveniles (initial weight: 21.1 ± 1.2g) were divided into four tanks per treatment, with 20 fish per tank. Fish were fed manually to satiety twice a day (8:00 and 17:00 h) for 8 weeks. Water parameters were maintained at a temperature of 27 ± 1°C, pH 7.4 ± 0.19 and dissolved oxygen of 6.0 ± 1.2 mg/L (mean ± standard error). Results demonstrated that the dietary supplementation of 0.03% AQUALYSO numerically improved the growth and feed conversion efficiency of largemouth bass. Fish fed the supplemented feed showed improvements in growth and feed efficiency by 11% and 5%, respectively, in relation to the control group (Figure 1A and 1B). These improvements highlight the effectiveness of LPLs in maximizing nutrient digestion and utilization even in feeds formulated with high inclusion of marine ingredients. AQUALYSO significantly reduced lipid accumulation in the mesentery and liver, indicating optimized lipid utilization (Figure 1C and 1D). Such effects are critical for maintaining the metabolic health of fish and improving energy availability for growth and feed efficiency. Trial 2. Efficacy of LPL supplementation in a plant-based feed fed to tilapia reared in low temperature conditions A second trial was conducted in collaboration with Testing Blue (Spain) to evaluate the efficacy of AQUALYSO (0.025%) supplementation to promote feed intake and growth performance of Nile tilapia (Oreochromis niloticus) fed a plant-based feed (Table 2) and reared at cold temperatures. Juvenile tilapia (initial weight: 52.32 ± 2.13g), were stocked in 150-L tanks in triplicate, with 16 fish per tank (initial density: 6.6 kg/m3). Fish were 500 450 400 350 300 250 200 150 100 50 0 1,6 1,4 1,2 1 0,8 0,6 0,4 0,2 0 0,9 0,88 0,86 0,84 0,82 0,8 0,78 0,76 6 5 4 3 2 1 0 Body weight gain (%) FCR MFI (%) Total lipid of liver (%) Control 11% 5% A C D * * B 0.03% AQUALYSO Control 0.03% AQUALYSO Control 0.03% AQUALYSO Control 0.03% AQUALYSO Figure 1. Trial 1: Comparing a fishmeal-based feed without (Control) and with 0.03% AQUALYSO supplementation. A: Body weight gain (%), B: feed conversion ratio (FCR), C: total fat in liver (%) and D: mesenteric fat index (MFI; %) (average ± standard error). Statistical differences are indicated by asterisk (One-way ANOVA, p < 0.05).
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