ISSUE FOCUS 60 FEED & ADDITIVE MAGAZINE September 2024 affecting aquaculture species. The results in figure 1 demonstrate the inhibitory efficacy of Orego-Stim Forte against all five pathogens when included at 2500ppm (2.5kg/t feed). Such findings highlight the potential for natural solutions, such as Orego-Stim Forte, to support fish and shrimp health, safeguard antibiotics, and protect against AMR. As well as proven efficacy in vitro, Orego-Stim Forte trials have demonstrated success in the production of shrimp when challenged with a disease. Vibrio parahaemolyticus is ubiquitous to many aquaculture environments and can cause diseases such as white faeces syndrome (WFS) and acute hepatopancreatic necrotic disease (AHPND) in L. vannamei, resulting in significant losses for the shrimp farming industry. An independent trial, conducted by Nong Lam University, in Vietnam, used 20 tanks containing 80 shrimp per tank with four replicates. At the end of an 8-week feeding period, 20 shrimp from each treatment were randomly selected and challenged with V. parahaemolyticus. Mortality rates were recorded for two weeks and at the end of the experiment total haematocyte count (THC), phenoloxidase activity and total Vibrio count were determined. In figure 2, the mortality of L. vannamei challenged with V. parahaemolyticus showed a decreasing tendency with in0 5 10 15 20 A. salmonicida V. anguillarum V. parahaemolyticus F. psychrophilum P. anguilliseptica Mean Clearance Zones (mm) 2500ppm 2250ppm 2000ppm 1750ppm 1500ppm 1250ppm Figure 1. Mean clearance zones (mm) of Orego-Stim Forte against common pathogenic bacteria affecting aquaculture species. Time post challenge (hours) Figure 2. Post challenge cumulative mortality of shrimp fed one of three dietary groups; D1 (Control), D2 (Orego-Stim Forte at 1kg/t), and D3 (Orego-Stim Forte at 3.5kg/t).
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