ISSUE FOCUS 48 FEED & ADDITIVE MAGAZINE September 2024 terocytes. Furthermore, the protein abundance of amino acid transporters was enhanced which may lead to improved amino acid transport and absorption in animals (Sun et al., 2017). In broilers, the effects of a combination of cinnamon and garlic powder (Excential Alliin Plus, Orffa Additives B.V.) was evaluated on reducing crop, cecal and environmental prevalence of Salmonella at the end of the rearing period. A total of 216 male broilers at 35 days of age were allocated to one of three treatments, each with 6 replicates: • Control with no additive (CC) • Garlic and cinnamon blend added to mash feed at 900 ppm (AP-F) • Garlic and cinnamon blend added to drinking water at 1 g/L (AP-W) At 7 days post placement, the birds were challenged with Salmonella Typhimurium (STM) via oral gavage. The effect of Excential Alliin Plus on the STM challenge was measured in the litter of each pen and sampled by using intermittently steppedon drag swabs. Litter was classed as ‘high prevalence’ if STM recovery was higher than 102 CFU/ mL via direct plating. If STM was recovered only after enrichment, the litter samples were classed as ‘low prevalence’. STM was also measured in cecum and crop samples. Although all litter tested positive for STM, the AP-W treatment had the lowest prevalence at 6 days post challenge as there was no recovery of STM with direct plating (Table 1). With regards to the cecal recovery of STM at 7 days post challenge, a reduction of over 1 log CFU was observed for the AP-F (1.49) and AP-W treatment (1.85) compared to the control treatment (3.07). At 12 days post challenge a reduction in the CFU of STM of around 0.6 log was observed in the ceca of birds receiving the garlic and cinnamon blend via feed or drinking water compared to the control. Also in the crop a reduction in STM of over 1 log CFU was observed at 12 days post challenge with AP-W (0.23) and AP-F (0.15) compared to the control (1.39) (Figure 3). These results suggest that the bioactive compounds in garlic and cinnamon may help to reduce the prevalence of environmental and crop/cecal colonized Salmonella in chickens prior to processing, and may therefore reduce the risk of transmission to poultry meat for consumption. High prevalence Treatments Low prevalence Table 1. Number of pens within each treatment group with a high or low prevalence of STM 6 days post infection. CC AP-F AP-W 4 3 6 2 3 0
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