ARTICLE 72 FEED & ADDITIVE MAGAZINE July 2024 Difference betweem treatments (MBP - CON) 6 5 4 3 2 1 0 -0.1 -0.05 0 0.05 0.1 - log10 (p-value for the difference) with an advanced microbiome profiling tool called Galleon™. It uses a microarray assay to process samples, compare the results with a database, and using machine learning algorithms predict biomarkers related to key performance parameters. One of the advantages of using Galleon™ is that the sample collection is non-invasive (cloacal swabs) as there is no need to sacrifice birds. So, multiple samples can be collected for different time points from the same bird. In the case of the four Dia-V™ MBPRO studies, a meta-analysis was conducted across the microbiome data from the studies. The results revealed that the birds fed Dia-V™ MBPRO had significantly (P<0.05) higher abundance of butyrate producing bacteria (Lachnospiraceae) across all ages sampled (cloaca swabs, day 21 and 35) as shown in Figure 3. This is indicative of an accelerated maturation of gut microbiome to form a more resilient microbial population at an early age of these broilers. Butyric acid has several beneficial effects such as strengthening of the gut epithelial barrier and reducing inflammation. So, a high abundance of butyric acid producing bacteria in the gut in case of the Dia-V™ MBPRO birds indicates that it supports a healthy gut. In addition to the increased abundance of butyrate producing bacteria, it was observed in this study that there was a trend (P>0.05) in reduction of some of the undesirable bacteria such as Clostridium perfringens and Listeria (Figure 4). CONCLUSIONS Understanding the modes of action of various feed ingredients, identifying the gaps, and developing Footnote: In the above graph, positive values on x-axis indicate higher abundance of that bacteria in MBP group and negative values on x-axis indicate lower abundance in MBP group compared to CON group. When the corresponding y-axis values get above the red dotted line, the MBP-CON difference is statistically significant (significance cut-off or threshold). Figure 3. Volcano plot: Comparison of Control and MBPro across four different studies and two different time points (21 and 35 days)
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