ISSUE FOCUS FEED & ADDITIVE MAGAZINE May 2024 55 First of all, the test results showed a clear dose-response in the reduction of intra-cellular ROS content up to 1,000 ppm ELIFE®. Secondly, with regards to VE replacement, the decreased intra-cellular ROS presence was similar between the Trolox® treatment (equal to 1,100 ppm synthetic VE50 ADS.) and the 500 ppm ELIFE® group. As a result, a dosage ratio of at least 1:2 between the antioxidant blend and VE50 ADS. could be concluded. Such an antioxidant equivalence would signify a matrix value of min. 1,000,000 IU/kg product for partial VE replacement (1 mg ELIFE® antioxidant blend = 2 mg VE50 ADS. = 1 IU). PRACTICAL EXAMPLES OF PROFITABLE & SAFE REPLACEMENT In monogastric diets, no replacement is allowed by the antioxidant blend at VE levels up to 20 IU/ kg feed (i.e. minimum nutritional requirement + safety margin). In subspecies with a higher VE need, like sows or breeder chickens, this lower limit lies at a higher level. Above this threshold, the replacement rate of VE50 ADS. by the antioxidant blend is only gradually increased with dietary VE level. Such safe substitution advice is key, so that the min. physiological requirement for VE, as set by independent animal research institutes (like NRC, INRA or FEDNA), is never compromised. Below Table 1 shows some replacement examples and their indicative feed cost savings with ELIFE® in different animal applications (calculated with an end user price of 7.5 USD/kg for VE50 ADS.). CONCLUSIONS Due to their chemical nature, polyphenols are more versatile antioxidant molecules compared to Figure 5. Dose-response decrease in ROS content inside animal gut cells after antioxidant administration, with (solid bars) or without (dotted bars) an oxidative challenge. Adapted from Ayuso et al. (2020)1. * statistically significant differences (at p<0.05) in ROS content between non-stressed and stressed cells (i.e. stressor effect) # statistically significant decrease (at p<0.05) in ROS content after treating cells with Trolox® water-soluble Vit. E or with ELIFE®, when compared to untreated control cells (i.e. antioxidant effect)
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