Feed & Additive Magazine Issue 36 January 2024

A new year means new beginnings and new opportunities. For the animal nutrition industry, this may mean reviewing current practices and adopting more sustainable, ethical and efficient methods. The challenges that the animal nutrition sector has had to overcome in recent years have increased considerably. But the industry is ready to meet these challenges with an extremely strong capacity for adaptation and innovation. So, as we enter the new year, it makes perfect sense for the industry to use these past experiences and challenges as a stepping stone to build a better future. Because transformation is inevitable for the industry. It is obvious that at the center of this transformation there will be more sustainable feeding methods and lower environmental impact for animal health and welfare. Antibiotic reduction is also an important part of the sustainability strategy and this transformation process. The use of antibiotics, which are used to protect animal health and prevent diseases, as growth promoters or excessive use in treatment and care, causes antibiotic resistance to increase day by day. This is an extremely serious potential threat to human health. Therefore, keeping the use of antibiotics in the livestock sector at the lowest possible level is vital to prevent the spread of this resistance. Of course, there are ways to reduce the use of antibiotics in the livestock sector. For example, one of the most important is to prevent diseases before they start. Strengthening the immunity of animals and providing them with a healthy environment can prevent the start and spread of diseases and reduce the need for antibiotics. In case of illness, alternative treatments are offered as an important solution. For this purpose, many alternatives such as probiotics, prebiotics, phytogenic feed additives are offered today. If you are looking for antibiotic reduction strategies, strengthening the immune system of animals and alternative solutions for treatment, you can review this issue of our magazine. Enjoy your reading! Wishing you a happy and peaceful New Year! See you in the next issue! Publisher Muhammed Akatay [email protected] Responsible Manager Özlem Akatay [email protected] Editor-in-Chief Derya Yıldız [email protected] Editors Bahadır Büyükkılıç Burak Varol [email protected] Design Alper Kandemir [email protected] Web Design Caner Cem Pulat [email protected] Proofreading Lisa Atakul [email protected] Circulation and Data Responsible Amine Şengün [email protected] Ataturk St. Metropol Istanbul Ste. Block C1, No:376 Atasehir, Istanbul/TURKEY Phone: +90 850 220 5064 E-Mail: [email protected] Web: www.feedandadditive.com Publication Type: Periodic (Monthly) EDITOR A new year and sustainable future - one step further... Derya YILDIZ © Three Plus Media | All rights reserved. Reproduction, in whole or in part, is prohibited without the written permission of the publisher. *DISCLAIMER: All views and ideas expressed in articles published in the magazine reflect the point of views of the author(s), not that of the Editor-in-Chief, Editors, Editorial Board or Publisher of Feed & Additive Magazine. The Editor-in-Chief, Editors, Editorial Board and Publisher assume no responsibility or liability in such cases. The publisher does not give any warranty and representation to the accuracy or completeness of the contents and data published in the magazine. All responsibility of the contents, articles and information published in the magazine belongs to the authors. The publisher and the authors cannot be held responsible for any damages resulting from the use of the whole or part of the content of the magazine. feedandadditive

Improve gut health and animal production with Presan® ANTIBIOTIC REDUCTION PROGRAMME Learn more, visit www.selko.com THE BENEFITS A farm’s financial results are highly dependent on animals having a stable, diverse microbiota and a strong gut wall. Presan® contains a powerful, integrated formula designed to help maintain the gut health status of animals and improves zootechnical performance. Improves animal performance Improves microbial balance Strengthens gut barrier integrity

CON T E N T S 20 ISSUE FOCUS 26 METABOLITES AND GUT MATURATION IN NEWLY WEANED PIGLETS Yvonne van der Horst, Selko Global & Tetske Hulshof, Trouw Nutrition 22 USE OF ADVANCED TECHNOLOGIES AND FEED ADDITIVES IN CONNECTING GUT MICROBIOME MATURATION TO BROILER PERFORMANCE Vivek Kuttappan, Cargill 42 ANTIMICROBIAL RESISTANCE IN SHRIMP FARMS Abisha Juliet Mary S J, TNJFU 30 BOOSTING CALF IMMUNITY THROUGH MCFA IN MOTHER’S DIET Frederik Gadeyne, Agrimprove 34 INTESTINAL HEALTH AND PATHOGEN RISK-REDUCTION BENEFITS ANTIMICROBIAL MANAGEMENT Célia G da Silva & Dr. Luiz Souza, ADM 40 WOAH CALLS FOR ANTIMICROBIALS NOT TO BE USED AS GROWTH PROMOTERS Anne-Laure Ledoux, Agrimprove 46 SUSTAINABLE ANIMAL HUSBANDRY Paulina Abramowicz-Pindor, AdiFeed SUSTAINABILITY ANTIBIOTIC-FREE PRODUCTION & IMMUNITY DEVELOPMENT

CON T E N T S Adisseo opens its new European R&I Centre, ELISE 8 Navigating poultry industry challenges 12 Novus tackles maximizing production efficiency for dairy farmers 78 Evonik develops microbial consortium to reduce antibiotics 17 Trouw Nutrition to share its scientific research at IPPE Expo 10 Willows Ingredients offers alternative feed ingredients to fish oil 68 NEWS GRAIN REPORTS GLOBAL SOYBEAN SUPPLY AND DEMAND & 2024 EXPECTATIONS 66 MARKET REPORTS GLOBAL FEED AMINO ACIDS MARKET 60 52 GROWING PROFITABLE HEIFERS: A PRODUCTIVE LIFE STORY Dr. Gavin Staley, Diamond V/Cargill 55 ROLE OF NON-STARCH POLYSACCHARIDES (NSP) AND INDIGESTIBLE OLIGOSACCHARIDES IN FISH NUTRITION Ranju Kumari, ICAR-Central Institute of Fisheries Education ARTICLES Methane-reducing solutions can transform cattle into climate champions 81 New FAO report maps pathways towards lower livestock emissions 83

NEWS 6 FEED & ADDITIVE MAGAZINE January 2024 ADM, one of the global leaders in human and animal nutrition, announced that it reached an agreement to acquire PT Trouw Nutrition Indonesia, a subsidiary of Nutreco and a leading provider of functional and nutritional solutions for livestock farming in Indonesia. Incorporated in 2007, PT Trouw Nutrition Indonesia is a leading premix manufacturer, providing innovative and comprehensive nutrition solutions for the animal industry. Their premix production facilities feature full automation solutions with intelligent process optimisation, and they are industry 4.0-ready with professional project management and execution. With the planned acquisition, ADM will be strengthening its premix and feed additives & ingredients (FA&I) business and strategically positioning itself to meet the anticipated market growth to sustain the rising demand for protein. The acquisition encompasses two premix production facilities—known as the Pasuruan site in Surabaya and the Cibitung site in Jakarta—as well as laboratories, warehouses, and offices across Indonesia. "This acquisition will complement our regional footprint and will represent a step forward in achieving our vision of leading the animal nutrition industry,” said Gerald Wilflingseder, president of ADM’s animal nutrition business in APAC. In addition, Dr. Pierre Domps, general manager of animal nutrition Indonesia at ADM, commented, “Our commitment extends beyond products and solutions; we strive to provide a spectrum of services. We will provide customised solutions and services backed by international technical expertise and support from our lab services.” Read more>> ADM acquires PT Trouw Nutrition Indonesia Arla Foods Ingredients harnesses upcycling power of insects Arla Foods Ingredients teamed up with ENORM, Northern Europe’s largest insect farm, in a partnership that will significantly reduce food waste. Delactosed permeate (DLP) is a residual dairy stream generated in large volumes during lactose production. Most DLP is currently used as material for biogas production, which is a less preferable option than reusing it for animal feed according to the food waste hierarchy set out in the EU’s Waste Framework Directive. Arla Foods Ingredients has long been investigating ways to address this challenge, but DLP’s composition has previously made it difficult to use in traditional animal feed. This is about to change thanks to the larvae of the black soldier fly. Currently used by ENORM for animal feed, they also offer huge potential as a healthy and sustainable source of protein for humans. Having developed the capacity to turn DLP into nutritious feed for larvae, Arla Foods Ingredients has been supplying ENORM with DLP for trial production for several years. However, ENORM opened a major new facility on December 6 in Flemming, Denmark, and is gearing up for full-scale production. As a result, the amount of DLP that Arla Foods Ingredients supplies to ENORM will increase substantially – to 15 truckloads a week in early 2024. When the new facility is up and running, it will be able to produce 100 tonnes of larvae daily – enough for more than 10,000 tonnes of insect meal. Read more>>

NEWS FEED & ADDITIVE MAGAZINE January 2024 7 The Dutch Denkavit Group and UK-based Volac International Ltd. reached an agreement for the acquisition by Denkavit of the Volac Milk Replacer business. This acquisition was a strategic decision for both parties, based on a strong wish to work with a partner with similar ambition and values to take the Volac Milk Replacer business to the next level. “Our Milk Replacer business is something we can be hugely proud of. We want that business to continue to flourish. As our portfolio of products in Volac Animal Nutrition expands further into the feed additives area following the successful acquisition of Micron Bio-Systems in 2021, we want to build on the synergies this brings. We have made the decision to sell our Milk Replacer business to a leading player in that market who can give it the focus it deserves," said David Neville, CEO of Volac International Ltd. "It was hugely important to us to sell to a company with similar ambition and values. We have enjoyed a healthy relationship with Denkavit over the years and look forward to this new chapter and seeing them take the Milk Replacer business to the next level.” Erik Buys, CEO of Denkavit, added, “We are very excited to announce this important step for us. The Volac products impress us, and we believe them to be a valuable addition to our portfolio, as they are renowned for superior quality and therefore fit seamlessly into our group. This acquisition strongly supports our ambition for global leadership in the calf milk replacer industry, bringing us a strong position in the UK and Irish markets.” Products for the home markets of the UK and Ireland will continue to be supplied and blended by Volac as part of the deal, and as such, will remain partners in this regard. Read more>> dsm-firmenich, the leading innovator in nutrition, health, and beauty, announced that it received market authorisation for Bovaer® in the United Kingdom. Bovaer® is a feed additive that effectively and immediately reduces methane emissions from cattle, with an average reduction in dairy cows of 30%. This is the first authorization by the UK for a feed additive targeted at an environmental benefit. According to dsm-firmenich, with this latest authorisation, English, Welsh, and Scottish dairy farmers will now also have a scientifically proven solution available to lower their carbon footprint significantly and reliably. For the entire dairy value chain, including supporting processors, retailers, and the food services sector, it means scope 3 emissions of dairy can be lowered by 10-15% CO2 equivalents per litre of milk. Additionally, it will help the UK deliver on the commitments of the Global Methane Pledge and the Climate Change Act. “At dsm-firmenich, we are excited about gaining another market authorization this year. It demonstrates the global relevance and potential of Bovaer®. Personally, I’m exceptionally delighted, as we are building a world-scale production plant for Bovaer® in the UK to help support global sales. Construction on the new plant is well underway in Dalry, Scotland, to become operational in the course of 2025,” said Mark van Nieuwland, Vice President Bovaer®. Read more>> Denkavit acquires Volac Milk Replacer business dsm-firmenich receives UK approval for Bovaer

NEWS 8 FEED & ADDITIVE MAGAZINE January 2024 Adisseo, one of the major players in the global feed additives market, officially opened ELISE (for European Lab for Innovation, Science & Expertise) in Lyon, France, three years after the start of the project to gather Research and Innovation teams within a European research centre. In a new building designed according to the needs and constraints of R&I activities, ELISE now hosts Research and Innovation activities in the fields of process chemistry, process engineering, analysis, and nutrition research - that’s to say 100, people. The choice of this location near the city of Lyon, in the heart of the Chemistry Valley, close to universities, and connected to technological partners in the Auvergne-Rhône-Alpes region will encourage network projects and develop synergies. "This grouping of R&I teams will make it easier to adapt to increasingly rapid changes in our sector, to answer the challenges of tomorrow’s agriculture and food, and to attract the best researchers who will strengthen the teams’ skills in France," stated Adisseo. Read more>> Adisseo opens its new European R&I Centre, ELISE Anpario and Saife Vetmed India celebrate new partnership in India Anpario plc, an independent international manufacturer and distributor of natural animal feed additives, signed a new manufacturing partnership agreement with New Delhi-based Saife Vetmed India Pvt. Ltd. In the last 10 years, economic development in India has seen a regular year-on-year growth of 6% of GDP; consequently, the market is attracting significant interest from international producers of feed ingredients. India is the second largest producer of eggs in the world behind China, the eighth largest producer of chicken meat, the largest producer of milk, contributing 24% of all the milk produced in the world, and the second largest producer of shrimp behind Ecuador. Saife Vetmed, based in New Delhi, has successfully represented Anpario’s flagship product, Orego-Stim, in India since 2008. Orego-Stim, developed and manufactured by Anpario, is the leading phytogenic feed additive formulated from a unique source of 100% natural oregano essential oil and is proven to support profitability, health, and performance in all species, according to the company. Changes to production practices and a greater focus on sustainable solutions to drive healthy, profitable growth, combined with improved access to the market over the last 2 years, have almost tripled Orego-Stim sales, and the partnership is ready to move to the next level. Andrew Jackson, Commercial Director at Anpario, commented, “We believe it is the right moment to increase our focus on India and capitalise on the opportunity such a significant market represents to Anpario.” “India is a significant opportunity, but it is also a considerable challenge. To be successful in such a goliath of a market, we need a stable and knowledgeable partner. In Saife Vetmed, we believe we have found that partner,” Jackson continued. Read more>>

NEWS FEED & ADDITIVE MAGAZINE January 2024 9 * Germination Optimisation Technology patents: US 9,447,376 & US 9,932,543 phileo-lesaffre.com FASTER STRONGER THE NEW POULTRY PROBIOTIC POWERED BY GO TECHNOLOGY* The information provided in this document is at the best of our knowledge, true and accurate. However, products must only be used in compliance with local laws and regulations and we cannot guarantee freedom of use for every intended application or country. PHS-P-BRO-AP-1804E1-en / Avalone

NEWS 10 FEED & ADDITIVE MAGAZINE January 2024 Trouw Nutrition, Nutreco's livestock feed business line, is sharing scientific research and unveiling nutritional technologies to support poultry production during the International Production & Processing Expo (IPPE), 30 Jan. to 1 Feb., in Atlanta, GA, U.S.A. Three International Poultry Scientific Forum presentations and an IPPE TECHTalk offer new insights from poultry research initiatives, including trials studying the effects of phytogenic ingredients, trace mineral sources, and organic acid supplementation on flock performance and farm economics. Visitors to the Trouw Nutrition booth (A823) can also learn about advanced technologies to support the production of safe, high-quality feed. Remarking on Selko and Trouw Nutrition’s presence at IPPE, Jaco Eissen, Global Manager Monogastric at Trouw Nutrition, stated, “Poultry producers have to deal with continuously changing circumstances that are influencing their business in 2024. Science-based insights can help producers and nutritionists make decisions that benefit their flocks and their farms.” Read more>> Trouw Nutrition to share its scientific research at IPPE Expo Skretting, one of the world leaders in the manufacture and supply of aquaculture feeds, introduced Nutra Terra, a novelty in hatchery feed with documented high performance and reduced emissions. Trials show a reduction in emissions of phosphorus and zinc from faeces of up to 30%, while fish fed with Nutra Terra have significantly better growth and a lower feed factor. Nutra Terra represents Skretting's largest innovation in grower feed for juvenile fish in over a decade. All available knowledge and research have been gathered to develop a new and optimised feed recipe, according to the company's statement. The result is a feed that provides better performance while reducing emissions from the fish and making better use of raw materials in the feed. The company believes that the key to success lies in raw materials. Skretting explains that Nutra Terra contains smaller amounts of limited raw materials, such as fishmeal and phosphorus, compared to conventional hatchery feeds. Part of the fishmeal is replaced with insect protein, which is part of the natural diet of salmon in the wild during this life stage. "Validation trials show a 7% increase in growth and a 4% lower feed factor in fish fed with Nutra Terra. It is gratifying to see such good performance numbers while reducing the need for limited raw materials," says Marcus Søyland, Head of Land Based in Skretting Norway. Read more>> Skretting introduces Nutra Terra, new generation of hatchery feed

NEWS FEED & ADDITIVE MAGAZINE January 2024 11 Novozymes and Chr. Hansen announce name of future combined company Novozymes and Chr. Hansen announced their future name, ‘Novonesis’. The name reflects the beginning of an era of biosolutions where Novonesis will unleash the full potential of biological solutions and generate significant value for all stakeholders and society at large. The announcement of the name marks an important milestone towards uniting the two companies. “Novonesis reflects where we came from, what we can achieve, and what we will become together. We are dedicated to harnessing the transformative potential of biology. Building on our legacy of developing innovative biosolutions, we stand ready to unlock unprecedented opportunities," said Ester Baiget, President and CEO of Novozymes. "In Novonesis, we will unite the brightest minds and the best science and technology in the field to help customers and businesses prosper while enabling them solve some of the greatest challenges we all face. We are here to start an era of biosolutions. That is why we have chosen to call our new company Novonesis, which means ‘a new beginning’.” Cees de Jong, Chairman of Novozymes, added, “We developed the name Novonesis in close collaboration and dialogue between Novozymes and Chr. Hansen. It has been crucial to find a name that can be home to all our 10,000 employees, but even more importantly, the name should represent the future potential of biosolutions. We believe we have found that name in Novonesis. A name that reflects our immense commitment to making biological solutions play an even bigger role in our planet’s future.” Read more>> Supplant D Health and performance go hand in hand • Improved health status • Stable rumen environment • Smoother transition • Reduced somatic cell count agrimprove.com Aromabiotic® Cattle

NEWS 12 FEED & ADDITIVE MAGAZINE January 2024 NAVIGATING POULTRY INDUSTRY CHALLENGES: Cargill's Innovative Solutions Cargill, a prominent figure in the global poultry industry, stands out as a frontrunner navigating the industry's complexities through innovation and commitment. Yann Fournis, Cargill’s Gut Health and Performance Poultry Category Lead, emphasizes the company's dedication to supporting live poultry producers across the entire production spectrum, from conception to consumption. At the heart of Cargill's Animal Nutrition business lies a diverse array of feeds and feed additives, and analytical and digital solutions, all strategically crafted to enhance bird performance. Key among these are postbiotics, obtained from fermentation, which are crucial in nurturing a robust intestinal microflora, complementing nutritional support provided through their feeds. In response to the industry's rising demands and the call to reduce antibiotic usage, Cargill plays a pivotal role. Their portfolio of nutritional solutions aims to bolster bird health while helping lessen reliance on antibiotics. Innovative technologies and key ingredients also serve to support help decrease health challenges and boost bird resilience. Sustainability remains a key focus for Cargill in poultry production. Dia V™ MBPRO, an innovative product supporting feed conversion ratio (FCR), impacts weight gain and meat yield for broilers by enhancing nutrient absorption and microflora modulation. Central to Cargill's animal welfare approach are postbiotics that adhere to ISAPP standards, supporting four critical health pillars: acquired immunity, innate immunity, digestive health, and intestinal microbiota balance. Looking ahead to the 2024 International Production & Processing Expo (IPPE), Cargill plans to showcase an integrated portfolio model, spotlighting tailor-made solutions through innovative feed additives, technical services, and digital technologies, aimed at optimizing poultry production. The much-anticipated IPPE 2024 will witness the unveiling of REVEAL™ Layers, a groundbreaking patent-pending NIR technology empowering producers to assess real-time body composition in poultry layers. This innovation promises to enhance laying persistency and profitability. To gain deeper insights into Cargill's impactful solutions, industry professionals are invited to visit their booth at IPPE Hall A, booth A2233. Read more>> SPECIAL NEWS

NEWS FEED & ADDITIVE MAGAZINE January 2024 13 Prices in dairy commodity market to rise in 2024 According to Rabobank's report titled 'Global dairy quarterly Q4 2023-Shifting to the next phase of the cycle', as 2023 draws to a close, the global dairy market continues to walk a tightrope of limited “new” milk and sluggish demand. Looking back on 2023, it is a story of soft global dairy commodity pricing due to weaker underlying fundamentals. Milk supply growth around the globe was underwhelming in 2023, with a brief return to growth for three consecutive quarters before lower milk prices, elevated costs, and weather disruptions put the brakes back on. The global markets patiently awaited the rebalancing of the Chinese market, only to experience the second consecutive year of large shortfalls in net dairy imports, according to Rabobank. Rabobank forecasts that in 2024, the global market will transition to the next phase of the cycle. There is growing evidence that the bottom in the dairy commodity markets has passed, and the general trend is for prices to move higher through 2024. Milk supply growth will be sluggish in 2024 across most export regions. Stock levels in the export regions are comfortable but not burdensome. This means that international dairy buyers must keep a close watch on supply availability amid structural weaknesses in production growth in some export regions. The New Zealand seasonal flush has passed with modest growth, and markets await seasonal increases from the Northern Hemisphere in 1H 2024. The next phase of the demand story remains key to watch. It’s a complex story of high dairy inflation, broader cost-of-living issues, and weak consumer confidence remaining on the horizon. Sluggish underlying dairy demand and changes in consumer purchases are impacting volumes in some economies and channels. Demand settings are on the mend, but market uncertainty remains due to rising unemployment in some economies. Its ongoing impact on consumer purchasing power will be a watchword for 2024. Read more>> Cal-Maine Foods, Inc., the largest producer and distributor of fresh shell eggs in the United States, announced a definitive agreement to acquire a broiler processing plant, hatchery, and feed mill in Dexter, Missouri, that was recently closed by Tyson Foods, Inc. The company plans to repurpose the assets for use in egg and egg product production. Cal-Maine Foods expects to close the transaction in its third fiscal quarter. Cal-Maine Foods will initially convert the broiler processing plant to an egg-grading facility. The company is excited about the growth prospects of this new operation and, subject to the completion of the transaction, anticipates making additional investments in the facilities and community and creating new jobs. Potential future expansion includes egg product processing capabilities, such as hardcooked eggs. In connection with the acquisition, Cal-Maine Foods expects to enter into agreements with certain of Tyson’s former contract farmers to convert their operations to support Cal-Maine Foods’ cage-free, free-range, or pasture-raised egg production operations. Commenting on the announcement, Sherman Miller, president and chief executive officer of CalMaine Foods, Inc., stated, “We are pleased to announce the proposed acquisition of the assets of Tyson’s former broiler processing facility in Dexter, Missouri." Read more>> Cal-Maine Foods acquires Tyson Foods' shuttered broiler processing assets

NEWS 14 FEED & ADDITIVE MAGAZINE January 2024 Variable weather has once again dominated crop quality and mycotoxin contamination patterns in Europe, with a distinct split between northern and southern regions. The Alltech 2023 European Harvest Analysis has collected and assessed over 1,100 grain and forage samples from more than 20 different countries, and the results show an overall higher-risk mycotoxin year in Europe in 2023. While Europe enjoyed a reprieve this growing season from the extreme drought conditions of the past 2–3 years, rains that fell close to harvest in northern and western Europe caused delays in harvesting that created ideal conditions for mould and mycotoxin development. The Alltech European Harvest Analysis, a decade-long initiative, is a comprehensive step in understanding the complexities of new-crop quality, mycotoxin prevalence and the threat that mycotoxins pose to animals and producers. To determine the most accurate representation of mycotoxin risk across Europe, Alltech has again collaborated with SGS, a global leader in mycotoxin testing and certification. Their expert testing, along with testing by the independently accredited Alltech 37+® laboratory in Ireland, which can detect up to 54 individual mycotoxins, has captured a highly accurate and robust set of new-crop mycotoxin data across 20 countries in Europe. ‘’In contrast to recent years, it is excessive rain rather than drought that has been the primary driver of mycotoxin risk in Europe,” said Dr. Radka Borutova, European technical support manager with the Alltech Mycotoxin Management team. “The delayed harvest in northern and western regions has created particular problems in small grains and forages across this region, while further south, corn crops have fared much better than last year, although, as we always try to highlight, low risk does not mean no risk.’’ Read more>> Alltech Harvest Analysis: Weather conditions increase mycotoxin risk Hungarian insect producer Agroloop chosen Bühler’s sophisticated insect growth system for its industrial black soldier fly plant. The facility will produce up to 4,000 metric tonnes of animal feed ingredients per year and will be built in Üllő, Hungary. Swiss technology group Bühler will deliver its proven crate-based nursery and rearing technology, which will enable a quick ramp-up of the plant for commercial production. By the end of 2024, Agroloop plans to launch its first products, contributing to a more sustainable animal feed value chain. Following its foundation in 2017 and the successful operation of a pilot facility, Agroloop got the green light for the implementation of an industrial insect plant in Hungary in 2022. Now they have selected all execution partners and are working at full speed to construct the plant and get it operational. The insect facility will be realised in an existing building of the Aerozone Park in Üllő, which is located next to the Budapest Ferenc Liszt International Airport. The new plant, Agroloop, supported by Bühler insect technology expertise, will produce more than 25,000 metric tonnes of black soldier fly larvae that will be turned into sustainable feed ingredients for the pet food, aquaculture, and livestock sectors. The plant is only the first step in Agroloop’s stratHungarian insect producer chooses Bühler for industrial insect plant

NEWS FEED & ADDITIVE MAGAZINE January 2024 15 egy to make insect feed ingredients available for Central and Eastern European (CEE) agribusiness. “The abundance of food processing by-products presents a unique opportunity for Agroloop's multiplant rollout strategy in the CEE region. Leveraging our strategic partnership with the leading regional feed producer, UBM Group, we improve feed quality and sustainability by creating future-proof feed formulas. This enables Agroloop to focus on rapid expansion and solidifies our position as a key player in the region,” said István Nagy, co-founder and CEO of Agroloop. Andreas Baumann, Head of Market Segment Insect Technology at Bühler, added, “We are very proud to be part of this pioneering project for the CEE region and delighted to see that our solutions will contribute to more sustainable feed supply chains.” In the pursuit of sustainable and environmentally friendly practices, the CEE region is increasingly turning to alternative sources for feed ingredients. By incorporating insects into the feed supply chain, the region can address environmental concerns, reduce dependence on imported protein sources, and contribute to a circular economy approach. In addition, innovative feed formulations containing insects can optimise animal health and growth, thus leading to more efficient livestock production systems. Read more>> The University of Leeds and Entocycle unveiled a new £430,000 research programme dedicated to developing the protocols and codes of practice to unlock the growth of the UK insect farming industry and its potential to decarbonise livestock production. According to the statement, the UK insect farming industry is currently constrained by strict legislation on the use of insect protein in animal feed and a lack of protocols and standards for insect-rearing facilities. Furthermore, current UK and EU legislation prohibits the use of livestock manure and slurry as feedstock for insects, restricting its potential to reduce the growing problem of agricultural waste and the associated environmental pollution. Currently, the feedstocks allowed to breed and feed insects are primarily of vegetal origin. Examples include vegetables not meeting specific supermarket standards, byproducts from vegetable processing such as potato or carrot peelings, or spent grains from beer or spirits production. Animal products are not permitted, except dairy products and eggs. The project, named InSAFE, is funded by Innovate UK and BBSRC as part of the Novel Low Emission Food Production Systems competition and will evaluate the use of non-permissive feedstocks, including pig slurry, chicken manure, and sewage sludge, to raise black soldier fly (BSF). Protocols, codes of practice, and Food Safety and Quality standards will also be developed for a range of production scales, from small lab-scale units to commercial farms. A new, state-of-the-art insect-rearing demonstrator facility, developed and installed by the UK’s leading insect technology company Entocycle, will house the research and is situated at the National Pig Centre on the University of Leeds’ research farm in Yorkshire. Read more>> Leeds University and Entocycle to evaluate use of non-permissive feedstocks in UK to raise BSF

NEWS 16 FEED & ADDITIVE MAGAZINE January 2024 Trouw Nutrition and the Global Dairy Platform (GDP) agreed to collaborate on a global pre-competitive Animal Nutrition Workstream to help support and guide research on innovative solutions to decarbonise the dairy sector. Developed as part of the Pathways to Dairy Net Zero (P2DNZ) initiative, the task force will provide leadership in the dairy nutrition space, focusing on opportunities to minimise the environmental impact of dairy production through innovative forage, feed, and feeding developments and technologies. To launch this partnership, GDP and Trouw Nutrition will host two P2DNZ webinars on January 17. The GDP is a dairy value chain organisation whose members collaborate pre-competitively to address issues important to the industry. GDP developed the P2DNZ initiative, a growing global movement dedicated to reducing dairy’s greenhouse gas emissions over the next 30 years by accelerating action against climate change throughout the dairy sector. Over 200 organisations, representing nearly 40% of global milk production, have already signed on. JJ Degan, Ruminant Manager of Global Strategic Marketing at Trouw Nutrition, said: “At Trouw Nutrition, we are committed to investing in improving the future of dairy farming. We believe that the P2DNZ’s collaborative and pre-competitive approach will enable us to collectively identify game-changing opportunities that will help us move the needle on the urgent task of reducing the dairy sector's climate impact while also increasing the supply and access to nutritious dairy foods for a growing global population." The dairy sector supports one billion people’s livelihoods while also providing nutritious dairy products to billions across the globe. It will play a critical role in helping to achieve Nutreco’s purpose of Feeding the Future: ensuring the 10 billion people on the planet in 2050 have enough high-quality nutrients to thrive. But at the same time, dairy is a significant contributor to greenhouse gas emissions, representing 2.5% of global emissions, so lowering its impact is an urgent priority. Read more>> Trouw Nutrition to chair Animal Nutrition Workstream for dairy net zero Enorm Biofactory inaugurated the largest insect farm in Northern Europe in Hvirring, Denmark. Enorm aims to produce 100 tonnes of larvae per day on its new farm. On the new 22,000-square-meter farm, black soldier fly larvae (BSFL) will be bred and processed into insect protein and insect oil. Much of the technology installed at Enorm Biofactory was supplied by Better Insect Solutions, a subsidiary of the Big Dutchman Group that specialises in complete solutions for insect farming. Better Insect Solutions provided the climate systems for the breeding and growing sections, air cleaning and heat recovery equipment, the liquid feeding system, and the crates for the larvae. Overall, around EUR 70 million was invested in the project. Northern Europe's largest insect farm opens in Denmark

NEWS FEED & ADDITIVE MAGAZINE January 2024 17 Evonik’s Biotech Hub is partnering with the Technical University of Munich (TUM) and RWTH Aachen to develop a novel bacterial consortium to strengthen the immune system of chickens and prevent colonization by pathogens of their intestinal tract. The aim is to enhance health and help reduce the use of antibiotics. Evonik is both a partner in the three-year joint Chicken Synthetic Microbiota (ChiSYN) project and the project coordinator. The total project volume is over €2 million and is funded by the project partners on a pro-rata basis and by the Federal Ministry of Food and Agriculture (BMEL). “For our health, we need healthy food, and for that, we need healthy animals,” says Christoph Kobler, who heads the Biotech Hub. “Our innovative microbial consortium contributes to both animal health and sustainable human nutrition.” The aim of the project partners is to develop a prototype feed additive that makes sure that “beneficial microorganisms” colonise the gut of chicks. To achieve this, bacteria that strengthen the immune system and make colonisation by pathogens more difficult are selected from a broad population of chickens. The project partners then combine these microorganisms to create a novel consortium. “In the development of this bacterial consortium, we benefit from our experience of gut simulation and the successful development of probiotics for livestock farming,” says Stefan Pelzer, who heads the In Silico & Target Systems research unit at the Biotech Hub. Pelzer and his team have been working with the dynamic chicken gut simulation model “DAISy” since 2018. They use the findings to develop new gut health products and disruptive, high-precision nutritional concepts that take account of gut bacteria for the first time. In this project, Evonik’s experience in industrial biotechnology is perfectly complemented by the skillsets of its strategic partners at the universities in Aachen and Munich. Read more>> Evonik develops microbial consortium to reduce antibiotics “Enorm Biofactory is a milestone for insect farming in Europe, and we are delighted that our equipment has made such an important contribution to the success of this lighthouse project,” said Dr. Frank Hiller, CEO of Big Dutchman. “We value this cooperation very highly because the larvae of the black soldier fly have enormous potential. We believe that this alternative protein source can permanently replace a large part of the soy that is currently being imported to Europe.” Accordingly, Big Dutchman has bundled the group’s insect farming know-how into the subsidiary Better Insect Solutions, which was founded in 2020. Enorm Biofactory breeds black soldier flies that mate and lay eggs, from which the larvae hatch. The larvae’s main food source is residue from the regional food industry. After around 12 days, they are processed into insect oil and insect meal, products that have already shown promising results in connection with the management and health of pigs and poultry. The goal to produce 100 metric tonnes of larvae per day. Read more>>

NEWS 18 FEED & ADDITIVE MAGAZINE January 2024 FEFAC: Outlook for compound feed demand in 2024 remains uncertain European Compound Feed Manufacturers’ Federation (FEFAC) explained its EU compound feed production estimates for 2023 and market outlook for 2024. EU compound feed production (EU27) for farmed animals in 2023 is estimated at 144.3 million metric tonnes, reflecting a 2% decrease compared to 2022, according to data forecasts provided by FEFAC members. The EU feed market in 2023 reflects continued political and market crisis management pressures and a growing demand for providing sustainable feed solutions to address market dynamics and regulatory considerations. These trends are a response to the adverse impacts of climate change and animal diseases on the supply of raw materials, such as droughts and floods, and on animal production capacity, including Avian Influenza (AI) and African Swine Fever (ASF). Additionally, national policies ranging from greenhouse gas reduction goals to nitrate emission regulations have contributed to these shifts. Benson Hill, Inc., self-described as an ag tech company unlocking the natural genetic diversity of plants, announced that recent advances in its soybean breeding programme will drive the doubling of its seed portfolio by 2025. The latest field evaluations on Benson Hill’s third generation of Ultra High Protein Low Oligosaccharides, non-GMO soybean varieties, showed protein gains of 2% over the previous generation and achieved a yield gap of only 3 to 5 bushels per acre, compared with commodity GMO soybeans. “We’ve successfully demonstrated that CropOS®, our AI-based prediction and data insights platform, can drive our predictive breeding efforts and give us a step-change forward on multiple traits like protein and yield,” said Jason Bull, Chief Technology Officer of Benson Hill. “We are now seeing massive gains in the field that minimise the tradeoff between yield and protein, surpassing expectations from when we began building on the high-protein soybean genetics we acquired in 2019.” “What this means for the industry is that we’re accelerating our speed to market with de-risked, outcome-based products in record time,” Bull added. “We expect to expand our portfolio of seed innovations again in 2025 to offer two dozen varieties that encompass protein, lower-indigestible sugars, and quality oil.” Benson Hill Chief Executive Officer Deanie Elsner also announced today at FARMCON 2024 an expansion of its commercially available soybean portfolio for 2024, adding five value-added varieties to its lineup. Benson Hill previously offered about a dozen soybean seed varieties that deliver ultra-high protein, high-oleic and low-linoleic oils, and low-oligosaccharide quality traits. Read more>> Benson Hill introduces five 2024 soybean varieties

NEWS FEED & ADDITIVE MAGAZINE January 2024 19 Moreover, shifts in production methods, as well as reduced or shifting demand due to changing consumer preferences (the impact of food price inflation), are affecting compound feed production differentially across Member States. While countries such as Germany, Ireland, Denmark, and Hungary have witnessed approximately a 5% decline in feed production, other countries like Austria, Bulgaria, Italy, and Romania have experienced a modest increase. The remaining Member States have either marginally decreased their feed production or maintained it at a level similar to the previous year. Regarding the outlook for compound feed demand in 2024, the scenario remains uncertain. Key factors, such as the impact of animal diseases, economic uncertainty, persisting high food price inflation, ongoing weather irregularities, and the increased imports of poultry meat products from Ukraine, are affecting local production. The influence of "green and animal welfare" policies is expected to adversely impact the market outlook for livestock and feed production, although costs for key feed materials, mainly feed cereals, have fallen back to levels before the Russian invasion of Ukraine. Read more>> Novus shares innovation supporting poultry industry at IPPE The Novus experts will present four abstracts as part of the International Poultry Scientific Forum (IPSF), which is held in conjunction with IPPE. The poster and oral presentations take place January 29-30, 2024, at the Georgia World Congress Center. “IPSF is the premiere event to learn the latest research on environmental management, nutrition, physiology, pathology, and disease,” says Gonzalo Prat, Novus senior director and managing director for the Americas. “We are honoured to participate in the forum to share how intelligent nutrition is supporting bird health and producer goals.” Dr. Zavarize said the abstracts from Novus showcase the importance of highly bioavailable trace minerals in poultry production. “The poultry industry recognises that trace minerals support health and performance, but not all minerals are the same,” she says. “Research like this showcases the difference a mineral source can make, both on its own and in combination with other products.” Read more>> Novus shares hatchability insights at IPPE’s TECHTalks Hatchability has become a top concern for the broiler breeder industry in the United States. Investigating the cause and how producers can support fertility and hatch are the focus of a presentation by Novus during the TECHTalks at IPPE. “In recent years, the U.S. has seen some of the worst hatchability numbers since the late 1980s. The U.S. Department of Agriculture in October 2023 reported average hatchability at just 80.4%,” says Hugo Romero, Ph.D., Novus global poultry technology executive manager, who will present the talk. “During this presentation I’ll examine the potential causes for this phenomenon and how adjustments to the ration can help combat it.” Dr. Romero’s presentation for the TECHTalks will be from 11:3011:50 a.m. EST on Tuesday, January 30 in Hall A at Booth A244. Read more>> Hugo Romero

ISSUE FOCUS 20 FEED & ADDITIVE MAGAZINE January 2024 ANTIBIOTIC-FREE PRODUCTION & IMMUNITY DEVELOPMENT

ISSUE FOCUS FEED & ADDITIVE MAGAZINE January 2024 21 Use of advanced technologies and feed additives in connecting gut microbiome maturation to broiler performance Vivek Kuttappan, Cargill Boosting calf immunity through MCFA in mother’s diet Frederik Gadeyne, Agrimprove Metabolites and gut maturation in newly weaned piglets Yvonne van der Horst, Selko Global & Tetske Hulshof, Trouw Nutrition WOAH calls for antimicrobials not to be used as growth promoters Antimicrobial resistance in shrimp farms Abisha Juliet Mary S J, TNJFU Intestinal health and pathogen risk-reduction benefits antimicrobial management Célia G da Silva & Dr. Luiz Souza, ADM

ISSUE FOCUS 22 FEED & ADDITIVE MAGAZINE January 2024 “The postbiotics could promote butyric acid producing bacteria directly or through cross-feeding mechanisms, while the essential oil compounds could control harmful bacterial, resulting in higher growth of butyric acid producing bacteria. Overall, the synergistic action of postbiotics and essential oil compounds could accelerate the development of a balanced and resilient mature microbiota in birds leading to improved performance.” USE OF ADVANCED TECHNOLOGIES AND FEED ADDITIVES IN CONNECTING GUT MICROBIOME MATURATION TO BROILER PERFORMANCE Vivek Kuttappan, DVM/PhD Poultry Technology Lead – Postbiotics Cargill Poultry production is forecasted to lead the global meat production by 59% in the next decade to meet consumer needs and ensure global food security (OECD/FAO, 2022). The challenge will be to meet this target in a sustainable way, making the most of limited feed resources and without being overly reliant on antibiotic or chemical compounds. As feed represents the largest cost input in poultry production, optimizing nutrient utilization and digestive efficiency are critical success factors. With 109 to 1011 bacteria/gram of intestinal digesta, the gut microbiome is a complex system with potential impact on gut health and production performance in broilers. Due to that complexity, understanding the changes in the gut microbiome and how we can influence it to adapt to challenges could be overwhelming at times. The use of advanced technologies and interventions, providing practical insights into the gut microbiome analysis of broilers to help improve animal health, performance, and preharvest food safety, can be advantageous in addressing sustainable broiler production. THE CONCEPT OF GUT MICROBIOME MATURATION Considering the complexity of the gut microbiome and the associated variations, it is important to establish the key attributes of a healthy gut microbiome. Extensive analysis of gut microbiome in broilers from field trials, as well as control research trials, were conducted using a non-invasive advanced microarray tool coupled with artificial intelligence – Galleon™ Microbiome Intelligence – to identify biomarkers for performance, preharvest food safety, harmful bacteria, etc. Results revealed that the broiler gut microbiome undergoes a maturation process from a simple to a more diverse microbial population (Figure 1). During the first week of age, the poultry gut microbiome primarily contains lactate producing bacteria (Lactobacillaceae, Streptococcaceae etc.). The short chain fatty acid (SCFA) producing bacteria (Lachnospiraceae, Ruminococcaceae etc.) uses lactate as a substrate, and thus there is a cross-feeding mechanism between the groups. As a result, the SCFA-producing bacteria increases in abundance later and produces SCFA such as butyric

ISSUE FOCUS FEED & ADDITIVE MAGAZINE January 2024 23 acid. Butyric acid has been reported to have several benefits by improving the gut integrity, barrier function, and reducing inflammation. Overall, the maturation of the gut microbiome results in a more resilient microbiota which can withstand challenges and support the birds in better digestion as well as utilization of nutrients. In fact, birds are most vulnerable to challenges during the initial stages of this maturation process. Interestingly, a comparison of high versus low performing flocks (~300g difference in body weight) showed that high performing birds had a more mature microbiome at an earlier age. Moreover, this could have helped the high performing birds to be more resilient to various environmental challenges, and divert more nutrients towards production performance. Low performing flocks were characterized by a lower abundance of lactate-producing bacteria during the first 14 days of production, resulting in lower numbers of short chain fatty acid (SCFA) producing bacteria at 35 days of age. The low producing birds showed higher abundance of several opportunistic harmful bacteria (Proteobacteria such as E. coli, Salmonella etc.), resulting in increased bacteria and food safety risk. Based on these findings, dietary interventions can be designed and implemented with changes in raw materials, nutrient levels, and using specific feed additives to promote a more favorable microbiome maturation, while keeping harmful bacteria under control. INFLUENCING GUT MICROBIOME MATURATION USING FEED ADDITIVES a. Postbiotics Learnings from these field trials, with respect to maturation of the gut microbiome, were used to evaluate the microbiome modulation benefit of a postbiotic-based feed additive. Postbiotics are defined by the International Scientific Association for Probiotics and Prebiotics (ISAPP) as preparation of inanimate microorganisms and/or their components that confer a health benefit on the host. A metanalysis of nine trials was conducted comparing birds with and without dietary inclusion of postbiotics, focusing on the changes in microbiome using microarray assay and artificial intelligence biomarker identification. The artificial intelligence model generated from the analysis revealed that nine out of ten biomarkers at 14d of age from the birds were linked to lactate producing bacteria. Interestingly, the dietary inclusion of postbiotics resulted in significantly higher abundance of lactate producers at 14d, which promoted the growth of Lachnospiraceae (short chain fatty acid producers), indicative of an early maturation of gut microbiome compared to the respective no-additive control. b. Syngery between postbotics and essential oil compounds In addition to that, a unique combination of postbiotics with a proprietary blend of essential oil compounds was evaluated for gut microbiome modFigure 1. Healthy gut microbiome maturation profile in broilers

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