Feed & Additive Magazine Issue 33 October 2023

October 2023 Year: 3 Issue: 33 Feed Additive INTERNATIONAL MAGAZINE FOR ANIMAL FEED & ADDITIVES INDUSTRY Arno Duchateau, Impextraco Intestinal health as driver for an efficient animal production system Virginie Blanvillain, AB Vista Gut health status as a tool for evaluating animal robustness and resilience in commercial conditions Stella Child, Good Food Institute Europe Alternative proteins are not the only solution, but they have a clear role to play… www.feedandadditive.com Importance and Protection of Gut Health in Farm Animals The Impact of Cell and Plant-based Proteins on Livestock Sector

The need to feed the growing world population with sustainable resources is one of today’s main research topics. Aside from the efficient utilisation of existing food resources, there is also intensive work to create new food sources. Cellbased meat, also known as cultured meat produced from animal cells in a laboratory environment, is considered as one of these new food sources. Companies working on cell-based meat production aim to make meat consumption possible without the need for slaughtering and suffering animals. Of course, some very important issues such as less greenhouse gas emissions, less water consumption, less land use and less antibiotics compared to traditional animal meat are among the strong arguments used by this industry to express itself. You will be able to produce meat without slaughtering any animals, feed millions, maybe even billions of people with it, and while doing so, your environmental footprint will be much smaller than traditional livestock farming systems... Don't you think this seems extremely utopian in today's conditions? Time will tell us how utopian or not this is in reality. The industry is still at the beginning of the road and there are still many challenges to overcome. One of the most important challenges at the moment is high production costs. Because it would not be wrong to say that the current production costs are extremely utopian for an industrial production target and consumer preference. Another challenge is to prove that the environmental impact is really lower. There is some research on this, but more research is needed on the environmental impact of very large volumes of production. Also, the long-term health effects of these meats have not yet been seriously researched. In conclusion, as the industry develops and research increases, a balance may need to be struck between the advantages and disadvantages of cell-based meat. What is not utopian in this environment is the fact that many well-established companies are investing in this field. These investors include companies from the animal nutrition industry. While we cannot yet predict how this industry will evolve, it is important to keep a close eye on how it will affect traditional animal husbandry. Because the traditional livestock industry hosts an extremely large employment and economy and may need a radical transformation depending on the development of the cell-based meat industry. In this month's issue, we have brought together two different views on the impact and future of cell-based proteins, especially cell-based meat. We hope it will be informative... Now let's return to our main field, namely animal health and nutrition... This month, we have focused on a topic that we can also call the key to success in animal production, gut health. In the content of this issue, you can find various articles on research and practice in this field. We wish you a pleasant reading... See you in the next issue... Publisher Muhammed Akatay [email protected] Responsible Manager Özlem Akatay o[email protected] Editor-in-Chief Derya Yıldız [email protected] Editors Bahadır Büyükkılıç Burak Varol [email protected] Design Alper Kandemir [email protected] Web Design Caner Cem Pulat [email protected] Proofreading Lisa Atakul [email protected] Circulation and Data Responsible Amine Şengün [email protected] Ataturk St. Metropol Istanbul Ste. Block C1, No:376 Atasehir, Istanbul/TURKEY Phone: +90 850 220 5064 E-Mail: [email protected] Web: www.feedandadditive.com Publication Type: Periodic (Monthly) EDITOR Cell-based meat: The future? Utopia? Derya YILDIZ © Three Plus Media | All rights reserved. Reproduction, in whole or in part, is prohibited without the written permission of the publisher. *DISCLAIMER: All views and ideas expressed in articles published in the magazine reflect the point of views of the author(s), not that of the Editor-in-Chief, Editors, Editorial Board or Publisher of Feed & Additive Magazine. The Editor-in-Chief, Editors, Editorial Board and Publisher assume no responsibility or liability in such cases. The publisher does not give any warranty and representation to the accuracy or completeness of the contents and data published in the magazine. All responsibility of the contents, articles and information published in the magazine belongs to the authors. The publisher and the authors cannot be held responsible for any damages resulting from the use of the whole or part of the content of the magazine. feedandadditive

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60 THE FUTURE OF CULTURED MEAT AND ITS IMPACT ON LIVESTOCK Dr Jean-François Hocquette & Dr Sghaier Chriki & Dr Marie-Pierre Ellies-Oury, INRAE, France CON T E N T S SPECIAL STORY LifeStart Calf Symposium to highlight discovery transforming calf nutrition 8 Lallemand's experts offer deep diving into liquid feed hygiene 11 Alltech launches new prebiotic fiber for gut health 91 Anitox launches first-ever biosecurity audit app 16 Zivo Bioscience explains new trial results on its non-antibiotic product 92 Trials confirm efficacy of methane-reducing feed supplement 18 Tereos launches Profeed Advanced to support immune in aging dogs 87 NEWS REPORTS GLOBAL PET FOOD MARKET 76 65 ALTERNATIVE PROTEINS ARE NOT THE ONLY SOLUTION, BUT THEY HAVE A CLEAR ROLE TO PLAY… Stella Child, Good Food Institute Europe The Impact of Cell and Plant-based Proteins on Livestock Sector ARTICLES 68 HOW TO MITIGATE FORMULATION COSTS WHEN INGREDIENT PRICES ARE HIGH By EW Nutrition Technical Team 72 UNVEILING THE HIDDEN POTENTIAL OF STARCH IN AQUAFEEDS Kalaiselvan Pandi & Amit Ranjan, TNJFU

NEWS 6 FEED & ADDITIVE MAGAZINE October 2023 Adisseo, one of the world's leading feed additive companies, held an aqua seminar with the theme "Protect Your Margins" during TARS 2023 in Nusa Dua, Bali. The event brought together about 80 online and onsite participants from all over Asia Asia for an insightful day of learning and collaboration. According to the statement from Adisseo, aquaculture is a sector that continues to experience positive growth to meet world food demand. Besides farming techniques and the application of biosecurity, nutritional intake, and health are also factors that require consideration to support the improvement of the fish and shrimp performance in aquaculture. The strategic application of feed additives can be a way to help maintain business profits, namely by minimizing the risk of losses due to diseases and helping accelerate recovery in post-disease outbreaks. As a global company in the field of feed additives, Adisseo takes an active role in supporting the aquaculture sector by providing specific solutions for different aquaculture species, as well as services to increase effectiveness and efficiency in business. With this approach, it is hoped that it will encourage the fish and shrimp farming business in a sustainable and profitable manner. Kicking off the seminar, Alexander van Halteren, Global Commercial Manager, delivered a comprehensive update on the latest aquaculture and ingredient market trends. According to Alexander van Halteren's update, global disruptions in weather, freight, ingredients, energy, and inflation have created a perfect storm for the aquaculture industry. The outlook continues to be rather bleak, especially for the Asian shrimp market. Participants gained valuable insights into the evolving landscape of raw material supply and demand in the current geopolitical and environmental context. Dr. Waldo Nuez Ortin, Global Aqua R&D Manager, took participants on an intriguing journey into Adisseo’s latest aqua research findings. Exploring the depths of aquaculture innovation, from product conception to lab and field validation and elucidating the modes of action. Some of Adisseo’s latest aqua research strategies, such as co-infection challenge models, bioassays, parasite-animal models, and molecular transcriptomic methods were shared as examples. Attendees got a first-hand look at the research advancements that could help shape the industry’s future. Read more>> Grupo Almar, one of the top 5 shrimp producers in the world and dsm-firmenich, a global leader in health, nutrition, and beauty, announced a joint multi-year commitment to measure and improve Grupo Almar’s environmental footprint using Sustell™, dsm-firmenich’s intelligent sustainability service. Grupo Almar is partnering with dsm-firmenich to use Sustell™ across all their shrimp production as well as access dsm-firmenich’s sustainability experience and expertise. Full environmental footprinting of Grupo Almar’s shrimp production will commence starting January 2024 providing internationally valiAdisseo launches aqua seminar “Protect Your Margins” at TARS, Bali Grupo Almar and dsm-firmenich join forces for sustainable shrimp farming

NEWS FEED & ADDITIVE MAGAZINE October 2023 7 dated footprint results for the full year 2024 onwards. Grupo Almar wanted to formalise its sustainability journey and chose dsm-firmenich and Sustell™ to accurately measure and validate its carbon footprint as part of a full ISO 14040/44 compliant lifecycle assessment (LCA) based environmental footprint. The company looked at a multiple array of options and took inspiration from the salmon industry which has made significant progress in measuring and improving its environmental footprint thanks to tools like Sustell™. Wolfgang Harten, Chief Operating Officer of Grupo Almar, said: “Almar is making important and incremental progress to improve the sustainability of its shrimp production, by learning from others and by investing in technology – with tangible improvements already made. We are committed to continue moving forward, both in technology and in sustainability. Through our partnership with a global heavyweight in animal nutrition and health, dsm-firmenich, we have our sights set firmly on improving the sustainability of our industry. We chose Sustell™ to go beyond measurement by taking ownership of our full environmental footprint, managing our footprint 24/7 within our teams and organization.” Dr. David Nickell, Vice President Sustainability and Business Solutions, Animal Nutrition & Health at dsm-firmenich, added: “Grupo Almar is a first mover in the shrimp industry, with Sustell™ they will possess both a full, accurate environmental footprint and the scenario testing capabilities to map and make tangible improvements in sustainability. As seen from Sustell™ users around the world and across multiple industries, this then opens the door to greater production efficiency and farm profitability while unlocking new value opportunities, such as product eco-labelling, and access to sustainable finance.” Read more>> Novus International debuts a new program aimed at enhancing the financial success of broiler producers. The Scale Up™ Program for Broilers is focused on improving meat production and carcass quality through sustainably driven intelligent nutrition. “Intelligent nutrition is the combination of experienced people, insightful perspectives and smarter solutions that allow us to put more into everything we create,” said Dr. Gabriela Cardoso Dal Pont, the poultry solutions manager for Americas, Novus. “The Scale Up™ Program for Broilers focuses on three focus areas – performance, meat quality and carcass yield, and sustainability and animal welfare. By supporting these pillars through intelligent nutrition, we can positively impact profitability for the broiler producer.” According to the company's statement, Mintrex® Bis-Chelated Trace Minerals serve as the foundation for the program. Available in organic zinc, copper and manganese, the mineral is bonded to methionine source HMTBa, which protects the mineral as it travels through the digestive system to the site of absorption making it less susceptible to antagonism in the gut. This also means that over-supplementation, which is often found with inorganic trace minerals, is unnecessary. The program’s strategies, which are customized to meet the farm’s goals, include Mintrex® Zn trace mineral to support meat and carcass quality, Mintrex® Cu trace mineral to support performance, and the Reduce And Replace™ strategy, which is shown to reduce the excretion of zinc (up to 37%), copper (up to 41%) and manganese (up to 35 %). Read more>> Novus launches its new Scale Up Program for Broiler

NEWS 8 FEED & ADDITIVE MAGAZINE October 2023 ICCF publishes its guidance document on identification and characterization of feed ingredients The International Cooperation for Convergence of Technical Requirements for the Assessment of Feed Ingredients (ICCF) Steering Committee announced that it endorsed the guidance document on ‘Identification and characterization of feed ingredients’ during its meeting on the 05th of September 2023. In 2021, an Expert Working Group composed of dedicated regulatory authorities and industry experts from the founding ICCF members was created to provide recommendations on information necessary for the establishment of the identity and characterization of feed ingredients. This guidance document will support the applicants to gather the relevant information for that purpose. The guidance document is accompanied by a checklist to support the applicants, when collecting the data to be included in the submission package. Read more>> Trouw Nutrition, the livestock feed business of Nutreco, and the University of Nottingham School of Veterinary Medicine and Science are convening scientists, producers, nutritionists, and veterinarians from around the globe to share emerging research and novel discoveries that will nurture the future of tomorrow’s dairy herds. Expanding on the ruminant science and practical application shared at two prior symposiums, the 3rd LifeStart Calf Symposium will be held on 17-18 October at the East Midlands Conference Centre in Nottingham, United Kingdom. Presenters include several of the world’s most renowned authorities on calf nutrition, development, animal behavior, and farm management. A highlight of the symposium will be the unveiling of a discovery that offers the potential to transform a longstanding practice in calf nutrition. The event agenda includes optional on-farm workshops along with remarks from experts in calf development and management. Sessions will cover farm case studies, emerging insights into the optimal environmental conditions for calf rearing, and strategies for reducing risks on the farm. The application of precision nutrition to enhance calf-rearing success will set the stage for a major technology announcement. The 3rd LifeStart Calf Symposium will unveil a novel technology inspired by the discovery of how fat composition in milk provides natural metabolic signals to prepare the calf’s rumen and intestinal tract for starter feed. Scientists identified specific signals that play a role in avoiding digestive challenges such as acidosis, scouring, and the weaning slump. Affecting more than 18% of dairy replacement calves, digestive disorders are a major cause of illness leading to problems with early life development as well as health and performance problems later in life. “Milk plays a communication role beyond its great nutritional value,” said Javier Martin-Tereso, Ph.D, manager of the ruminant team at Trouw Nutrition Research Development. Read more>> LifeStart Calf Symposium to highlight discovery transforming calf nutrition

NEWS FEED & ADDITIVE MAGAZINE October 2023 9 At Phileo, we design efficient nutritional solutions to support a sustainable future within the pork industry. Our probiotic and active yeast fractions are proven to optimize post weaning transition till finishing enhance feed converion, and growth performance resulting in better meat yield and quality. Act with nature for animal care. Naturally improved animal performance

NEWS 10 FEED & ADDITIVE MAGAZINE October 2023 Bühler and NRGene collaborate to increase competitiveness of black soldier fly industry Bühler, the Swiss technology group, and NRGene Technologies Ltd., an Israeli genomics company, announced a strategic collaboration in the field of black soldier fly (BSF) genetics and processing. The two companies are combining their expertise to provide solutions and services to this rapidly evolving industry. The partnership between Bühler and NRGene offers BSF producers a comprehensive solution that incorporates Bühler's high-end equipment and engineering capabilities and NRGene's novel genetics and AI-based genomics tools. Production equipment and tailor made high-performance BSF strains are integrated into a customized solution that ensures cost-effective industrial scale production from the start. "The insect industry is still optimizing its production costs. Advances in insect genetics are key for achieving this. The benefit of genetics is already demonstrated in traditional agriculture. Combining the tailored insect strains with our advanced indusBioflytech constructions its second plant for BSF larvae production in Spain Bioflytech, one of Europe's leading companies in the production of industrial insect protein for animal feed, announced that work is progressing well on the plant it is building in Palas de Rei, in the Spanish autonomous region of Galicia. According to the company's statement, the rendering and drying plants are being set up, and in the coming weeks the first of the six fattening halls will start operations, each of which will produce 2,000 tonnes of fresh black soldier fly larvae per year, until reaching the maximum production capacity of this first phase, of 12,000 tonnes per year, during 1Q2024. In the coming years, further major investments are planned in this plant in order to reach 100,000 tonnes per year of black soldier fly larvae production. This new, second Bioflytech plant - the other is located in Fuente Álamo, in the Spanish region of Murcia - is equipped with a gas rendering installation designed by the company and unique in the market, a disruptive technology that will make it possible to manufacture tailor-made flours with specific percentages of protein and fats according to the customer's demands, and which is set to revolutionise the insect protein flour production sector. The Palas de Rei plant will also have a second conventional rendering line, which is currently in the testing phase and is expected to start production this November. Bioflytech was founded in 2012 as a biotechnology company linked to the University of Alicante. Six years later, the company Moira Capital acquired the majority of its capital, and at the end of 2019 moved its operations to Fuente Álamo. Bioflytech's first and so far, only plant in Fuente Alamo currently produces 4,000 tonnes of fresh larvae per year. One of the largest black soldier fly hatcheries in the world is located here, with a production of 180 kilos of black soldier fly eggs per month. Bioflytech's strategic plan includes an investment of 2.8 million euros this year in various improvements in this plant, with the aim, among others, of increasing the monthly egg production to 350 kg. Read more>>

NEWS FEED & ADDITIVE MAGAZINE October 2023 11 trial technology will bring a step change in the production of insects," says Andreas Baumann, Head of Market Segment Insect Technology at Bühler. "The overall solution will result in a high yield of superior products while ensuring an efficient use of raw materials, land, water, and energy. This makes the investment in insect production more attractive as it gives owners a crucial competitive advantage in the marketplace." "There is a growing demand for meat to feed carnivorous pets and farm animals. It is our belief that to realize BSF's huge potential as an alternative sustainable protein source used for feed, it is essential to integrate the expertise of various disciplines into this new industry, from genetics to engineering. We are excited to partner with Bühler as we move towards a cost-efficient industry scaleup," says Dr. Gil Ronen, NRGene's CEO and Founder. Insects are a healthy and sustainable source of protein for animal feed. They can contribute to a circular economy model as insects can be fed on organic waste, such as food leftovers and waste from agriculture, to produce feed for pets, fish, and livestock. The residues from insect farming, or frass, can in turn be used as a fertilizer, contributing to the zero-waste approach. Read more>> Lallemand Animal Nutrition's R&D and tech swine experts met with the world's leading experts in swine health and nutrition at the Pig Research Summit – THINK Piglet Health & Nutrition 2023, in Copenhagen, Denmark. In addition to exchanging about the latest trends and research in healthy pig nutrition, the team presented new data on the contribution of lactic acid bacteria Pediococcus acidilactici CNCM I-4622 to the hygiene of pig liquid feed. According to the company's statement, in Europe, liquid feed is particularly popular for swine in countries including Denmark, The Netherlands, Belgium, France, Germany, Italy and Sweden, where more than 60% of finishers — as well as the majority of sows — are fed liquid feed. "Hygiene is key in liquid feed systems: the goal is to keep the feed at a low pH in order to limit the growth of undesirable microorganisms. Additionally, a good feed hygiene has an effect on feed efficiency. In sows for example, higher hygiene in the liquid feed is expected to increase milk production thanks to improved feed utilization, leaving more energy available for milk production. Ensuring the sanitary quality of liquid feed and the feeding system itself is a key priority to raise quality pigs," said Lallemand's experts. The lactic acid bacteria strain Pediococcus acidilactici CNCM I-4622 (BACTOCELL), which was already marketed as a zootechnical feed additive with benefits on performance for piglets and fattening pigs, has recently been authorized in the European Union as a technological feed additive, in the categories of acidity regulator and hygiene condition enhancer. This lactic acid bacteria has been recognized for its potential to reduce pH and limit growth of coliform bacteria in liquid feeds, offering an effective tool to help ensure liquid feed hygiene and quality. Read more>> Lallemand's experts offer deep diving into liquid feed hygiene

NEWS 12 FEED & ADDITIVE MAGAZINE October 2023 Interagency Food Safety Analytics Collaboration (IFSAC) priorities for 2024-2028 have been published. As one of the partners to the world's leading feed and food producers, Anitox announced that it is committed to understanding how IFSAC's commitment to improving the coordination of federal food safety analytics efforts and addressing cross-cutting priorities for food safety data collection, analysis, and use impacts the industry. The attribution to foodborne diseases includes animal-derived food products where feeds used in the various species production systems have been identified as a vector for pathogens of human health significance such as Salmonella. The role of feed as a potential carrier for pathogens has been extensively studied, and animal feed has been associated with Salmonella contamination in more than 500 publications. Just this year, researchers at the University of Georgia conducted a comprehensive review of almost 40 publications spanning over 50 years, focusing on detecting and assessing the prevalence of Salmonella in broilers. The study analyzed data from over 40,000 samples to determine the relative contribution of various pre-harvest factors that affect Salmonella prevalence in broiler operations. The researchers identified feed as a significant factor, accounting for 4.8% of Salmonella positivity in broilers. Read more>> After a few years of absence due to COVID restrictions, Orffa Belgium NV was able to organize its seminar again for Flemish customers, a well anticipated event. According to the company's statement, the values of Orffa coincide very well with current challenges in the animal feed industry, including but not limited to: methane reduction, feed cost reduction, alternatives for antibiotics, reduction of ecological footprint, reduce energy costs during production, battle diseases through additives and improve worker’s health, the theme of this year’s seminar was “One Health”. Orffa Belgium invited several experts in their field to shed some light on different aspects. Dr. Ir. Alfons Jansman, senior researcher at Wageningen Livestock Research, focused on how to reduce nitrogen excretion by adjustments to the feed of pigs and poultry. Shannon Groen, researcher at Delft Solids Solutions, shed some light on the different techniques available to test dustiness of feed additives and feeds. Next to that she highlighted solutions to improve dustiness in order to safeguard worker’s health. Katrien D'hooghe, Managing Director of the Belgian Feed Association, gave some insight on the vast amount of topics that they are currently focusing on, to be a bridge between farmers and politics on all levels. Their 12 topics with goals to make the feed producing sector more sustainable by 2023, are very much in line with Orffa’s focus points to maintain the Ecovadis Gold recognition in years to come. Read more>> Anitox evaluates new priorities of IFSAC Orffa Belgium organizes “One Health” themed seminar for Flemish customers

NEWS FEED & ADDITIVE MAGAZINE October 2023 13 Novozymes and Bactolife join forces for health of piglets Novozymes and the Danish industrial biotech company, Bactolife, signed a joint development and commercialization agreement to finish development and launch the biosolution Ablacto+. The promising product can potentially stabilize the gut of piglets and reduce the severity of post weaning diarrhea (PWD). PWD is a major, global swine health challenge caused by E. coli infections in newly weaned pigs. Globally, PWD is estimated to lead to a loss of production of EUR 1-2 billion per year. Additionally, PWD is considered the main driver for antibiotic use in weaner pigs. “Post weaning diarrhea is one of the biggest challenges in the global swine industry. We are very excited to join this partnership with Bactolife. Together, we will be able to develop innovative biosolutions that can stabilize the gut of piglets, increase productivity, and significantly reduce the use of antibiotic use among pigs,” says Sofie Louise Trads, Director, Animal Health at Novozymes. “We are extremely happy to partner with Novozymes on this promising product. This is a major validation of Bactolife’s technology platform, and with a strong commercial partnership in the Animal Health space like Novozymes, we believe there is a significant business potential for both parties,” says Sebastian Søderberg, CEO at Bactolife. According to the company's statement, the product candidate, Ablacto+, is a functional feed additive which consists of binding proteins with a specific mode of action. Read more>> Supplant D MCFA blend for gut health support • Large antimicrobial spectrum • Supports the immune function by limiting white cell apoptosis • Better nutrient absorption through increase gut surface agrimprove.com Aromabiotic®

NEWS 14 FEED & ADDITIVE MAGAZINE October 2023 BASF offers Product Carbon Footprints for ingredients portfolio BASF calculated the Product Carbon Footprints (PCFs) for vitamins, carotenoids, beverage processing polymers, feed enzymes, and feed performance ingredients for the human and animal nutrition markets. The BASF methodology for calculating PCFs is certified by TÜV Rheinland according to the internationally recognized standard, ISO 14067:2018. The PCF sums up the total greenhouse gas emissions generated by a product over the different stages of its life cycle. This makes PCFs the central tool for creating transparency on emissions and identifying levers to reduce CO2 emissions. PCFs of BASF products comprise all product-related The European Soy Monitor 2021, containing an overview of estimations on the use of responsible and deforestation-free soy in European animal feed production in 2021, was published on 20 September 2023. The report points to 40% of soybean meal equivalent of total European soy consumption to be sourced according to the FEFAC Soy Sourcing Guidelines and 24% certified deforestation-free soy. The report also refers to FEFAC’s initial risk assessment which estimates that 93.9% of EU soy imports were sourced from low-deforestation regions, based on EU trade statistics and industry expert assessments. Since the publication of the first European Soy Monitor of 2018 estimations, the feed industry has demonstrated remarkable progress in responsible soy usage. The provisional data for 2021 indicates that FEFAC members reported an increase of 21% compared to 2018. Responsible soy, as defined by FEFAC, refers to soy provided through supplier and member schemes and programs that adhere to the criteria outlined in the FEFAC Soy Sourcing Guidelines. These guidelines encompass good agricultural practices, environmental considerations, and social responsibility. FEFAC President Pedro Cordero expressed his satisfaction with the continued progress, stating, "I am pleased with the continued positive trend that the European feed sector and its supply chain partners have been able to display as regards the industry's use of responsible soy. I am confident that we will make further progress on the mainstream market transition of responsible soy use from both imported and home-grown sources." Read more>> European feed industry continues its commitment to responsible soy

NEWS FEED & ADDITIVE MAGAZINE October 2023 15 greenhouse gas emissions that occur until the BASF product leaves the factory gate for the customer: from the purchased raw material to the use of energy in production processes. A benchmarking shows that the PCF for specific BASF vitamin A and E products is significantly better – at least 20 % – than the global market average PCF of the corresponding third-party products. BASF’s benchmark assessments have been conducted according to ISO 14044, ISO 14067, and GHG protocol standards. The independent certification by TÜV Rheinland confirms the calculation of the PCFs and market averages according to ISO 14067:2018, based on data provided by BASF. The certification is documented in a database (www. certipedia.com) and accessible to every customer upon request. “We are committed to supporting our customers to progress on their emission reduction targets. Our offer of specific vitamin A and E products with certified product carbon footprint better than the global market average is outstanding and we are proud to deliver this competitive PCF advantage to our customers,” said Julia Raquet, Senior Vice President of Global Business Unit of BASF Nutrition Ingredients. Read more>> Phileo by Lesaffre shown that feeding yeast (1.3) (1.6) beta-glucan Safglucan can help pigs cope better with the negative effect of enteric disorders caused by Lawsonia intracellularis. This is a new (patent pending) application for Safglucan to help the pork industry achieve profitable production while also reducing antimicrobial usage. Enteric disorders caused by L. intracellularis are often the source of reduced and heterogeneous weight gain, decreased feed efficiency, diarrhea, and even sudden death in pigs. Such disorders cause significant economic losses to the pork industry worldwide, even when no clinical signs of this condition are visible. At the same time, our knowledge of L. intracellularis is improving with nutritional solutions gaining attention as a means of boosting swine performance and well-being on farms that are exposed to L. intracellularis. In addition, in the context of rising worldwide concerns over antimicrobial resistance, finding alternatives to antimicrobial substances has become an on-the-ground need. L. intracellularis is ubiquitous on swine farms, often circulating easily among herds. Ultimately, almost all pigs on a L. intracellularis-positive farm will come into contact with the bacteria during their lifetime, often during their grower-finisher stage. Once in the intestine, the bacterium damages the intestinal wall. The gut microbiome balance is affected, nutrient absorption is damaged and resistance to other pathogenic threats is compromised. Phileo has confirmed, however, that the yeast-based solution Safglucan has great potential to improve intestinal resilience and pig performance, thus limiting the need for antimicrobials. Phileo has shown that supplementing feed with Safglucan during the growing and finishing phases significantly improves growth and reduces the proportion of slow-growing pigs. In addition, a favorable shift in the microbiome has also been observed in supplemented pigs. Intestinal resilience was significantly improved as shown by a reduction in average L. intracellularis shedding and/or the decreased prevalence of pigs shedding high numbers of bacteria. Read more>> Phileo: Safglucan mitigates negative impact of Lawsonia intracellularis

NEWS 16 FEED & ADDITIVE MAGAZINE October 2023 Forty companies from across the world registered for the F3 - Future of Fish Feed’s latest aquaculture feed contest — the F3 Krill Replacement Challenge. This current contest from the F3 initiative is designed to spark innovation for alternatives to krill in aquaculture feed. “We are thrilled by the vast amount of interest, both in terms of variety of products and from companies located all over the world,” said F3 Judge Kevin Fitzsimmons, a professor of environmental science at the University of Arizona. “It is gearing up to be a great competition and already highlights the fact that there are many viable replacements on the market already that we don’t need to fish krill from the wild for an ingredient in feed.” The companies that registered for the F3 Krill Replacement Challenge have a variety of high-quality products ranging from black soldier fly and algae to single-cell proteins. The next step is for the F3 judges to select the 10 companies that will compete in the 12-week comparative feeding trial in which each competitor’s marine-animal free product will be incorporated into an F3-designed, plant-based feed for Atlantic salmon (Salmo salar). To determine a winner of the $100,000 USD grand prize, the F3 judges will compare salmon weight gain, feed-conversion ratio, and survival observed for each krill replacement product. A diet containing krill meal is also included in the trial as a control. An announcement of the 10 companies selected for the feeding trial is expected in late October 2023. Read more>> Forty companies to compete in F3 Krill Replacement Challenge Anitox, one of the feed pathogen control specialists, launched the first free app to collect and present feed mill biosecurity audit records for all geographies and regulatory bodies. Dr. Enrique Montiel, Anitox’s Global Director of Nutrition and Live Production, explains: "Auditing feed mills and amassing the evidence and data needed to produce meaningful biosecurity programs can be a headache. For years our feed mill technologists have been walking facilities with customers, collecting evidence and preparing reports for regulatory and compliance bodies across the globe. That has become significantly easier, thanks to the new feed mill biosecurity audit app.” The Feed Mill Biosecurity Audit app enables mill managers and quality teams to establish a baseline for biosecurity standards by identifying areas of concern, implementing sound manufacturing practices and adhering to regulatory biosecurity requirements. Anitox is uniquely placed to produce the audit app, having a longstanding relationship with one of Europe’s leading independent feed microbial quality auditors. “Our team of feed milling technologists and technical specialists solve Salmonella, virus and other feed pathogen-related challenges on a daily basis; now that knowledge has been put the good use for the wider community. Having proven the technology with our loyal customers, we’ve decided it's too useful to keep to ourselves,” added Montiel. Read more>> Anitox launches first-ever biosecurity audit app

NEWS FEED & ADDITIVE MAGAZINE October 2023 17 FERMNUTRAL POSTBIOTICS EXPLORE THE POTENTIAL OF E-mail [email protected] Tel +86 371 8892 7707 Website www.cpbiolinkcn.com Address 904, Suite A, Building 3,Shenglong Plaza, DongfengSouthRoad,Zhengdong New Area, Zhengzhou, Henan, P. R. China GUT HEALTH IMMUNOREGULATION INHIBIT PATHOGENS INCREASE EGG / MILK PRODUCTION PROMOTE GROWTH POSTBIOTICS FERMNUTRAL

NEWS 18 FEED & ADDITIVE MAGAZINE October 2023 As one of the global leaders in animal nutrition and sustainable ag solutions, Alltech continues to partner with companies that share its commitment to Working Together for a Planet of Plenty™. Alltech announced that it is partnering with National Dairy Farms and Masakin Dairy Farms, two prominent companies owned by Emirates Food Industries (EFI), to bring leading-edge carbon footprint benchmarking and methane-reducing technologies to the Middle East. National and Masakin are known for their flagship milk brand, Hayatna, which has garnered widespread acclaim for its quality and freshness. Now, the two dairy giants are expanding their commitment to sustainability by embarking on a transformative journey to measure and mitigate their carbon footprints. With growing concerns about climate change, companies worldwide are under increasing pressure to adopt eco-conscious practices. As industry leaders, National and Masakin recognize the importance of curbing greenhouse gas emissions and setting new sustainability benchmarks. Through this Planet of Plenty partnership, National and Masakin will have access to a range of carbon footprint benchmarking technologies available through Alltech E-CO2, which will provide precise measurements of their environmental impact, enabling them to make data-driven decisions and set ambitious targets for reducing their carbon footprints. The dairies will also utilize nutritional technologies from Alltech that help lower methane emissions and enhance farm profitability by increasing feed efficiency and milk production. “The solidification of many years of collaboration with Emirates Food Industries in a Planet of Plenty Partnership represents the goals of the wider global Alltech family,” said Paul McVeigh, regional manager of Alltech Middle East. “This partnership illustrates what is possible in the Middle East region when companies join forces toward a single goal, and we are dedicated to supporting them with the best available nutritional technologies to achieve more milk while reducing their environmental footprint.” Read more>> Trials conducted at CERZOO, dairy farm of Università Cattolica del Sacro Cuore in Piacenza, Italy, confirm efficacy of Bovaer®, dsm-firmenich’s methane-reducing feed supplement for ruminants, achieving reductions close to 50% in methane emissions. Two on-farm trails conducted by the Università Cattolica del Sacro Cuore, the first of their kind in Italy, tested the efficacy of Bovaer® in ruminants consuming Italian dairy cow diets, that are rich in corn silage. At the recommended minimum dose, Bovaer® reduced enteric methane emissions by 44 to 50 percent while maintaining milk production and composition, milk fatty acids profile, animal health and welfare, and feed efficiency. These positive results, which follow more than 60 successful trials in 17 other countries, demonstrate once again that Bovaer® is highly effective in reducing methane emissions in dairy cows. dsm-firmenich, innovators in nutrition, health, and beauty and Parmalat, a leader in the Italian milk market and part of the Lactalis Group, announced that UniverAlltech and Emirates Food Industries embark on Planet of Plenty™ partnership in the Middle East Trials confirm efficacy of methane-reducing feed supplement

NEWS FEED & ADDITIVE MAGAZINE October 2023 19 sità Cattolica has concluded two highly successful trials of Bovaer®, dsm-firmenich’s methane-reducing feed supplement for ruminants. Reducing methane emissions is crucial to keep global warming below the 1.5°C target outlined in the Paris Agreement. Over 150 countries have signed the Global Methane Pledge, committing to reduce global methane emissions by at least 30 percent by 2030. To date, Bovaer® has helped save over 50,000 tons of CO2e by reducing methane emissions from dairy and beef cattle in its trials, pilots and implementations around the globe, making a vital and growing contribution to the realization of global climate targets. Mark van Nieuwland, Vice President Bovaer® at dsm-firmenich, said: “There’s no time to lose when it comes to reducing greenhouse gas emissions. Cutting methane emissions is the fastest way to combat global warming and will be high on the agenda at this year’s COP 28 in Dubai. I’m proud that dsm-firmenich can offer dairy farmers a solution that will contribute to tackling one of the greatest challenges of our time. Collaboration, new ways of thinking, and pioneering innovations are all crucial to making dairy farming more sustainable.” Read more>> CH4 Global and Lotte to provide methane-reducing feed supplement to 4 million cattle with CH4 Global, Inc., a climate tech company on the path to scalable and profitable production of products that enable the food industry value chain to radically reduce GHG emissions, announced partnership with multinational conglomerate, Lotte International. The intent of the strategic partnership is to bring Methane Tamer™, ruminant enteric methane-reduction technology, to South Korea. CH4 Global and Lotte International are currently advancing studies for regulatory approval in South Korea in consultation with the government. The partnership includes the initial supply to the Lotte-owned Australian feedlot, Sandalwood, for up to seven thousand head of cattle, primarily Wagyu Beef. As CH4 Global's exclusive partner in South Korea, Lotte will provide up to four million cattle with the enteric methane-reducing feed supplement. The climate impact, from reduced emissions, for four million cattle would be as much as ten million metric tons, or a conservative estimate of seven million metric tons of CO2-e per year (GWP100). The target launch is 2025, with Lotte leading commercialization and CH4 Global manufacturing and supply product. "As a company that operates a beef business in Australia, reducing methane gas is a very important ESG issue. Therefore, we are actively working together with CH4 to reduce methane gas generated at Sandalwood and supply low-carbon beef in the market. In addition, we are working to introduce CH4 Global products in Korea to support the Korean government's goal of reducing methane gas emissions," said Ki-ho Jeong, CEO of Lotte International. "From the start, CH4 Global has been focused on developing the products and partnerships that enable us to reach our critical goal of reducing methane emissions on a global scale. This partnership with Lotte is another step in the direction of achieving our vision and putting our groundbreaking feed additive in the hands of farmers and food producers worldwide," said Steve Meller, CEO and co-founder of CH4 Global. Read more>>


ISSUE FOCUS FEED & ADDITIVE MAGAZINE October 2023 21 Gut health and mycotoxins: What do we know so far? Dr. Alexandra Weaver & Dr. Radka Borutova, Alltech The role of postbiotics in promoting gut health in poultry Vivek Kuttappan, Cargill Gut health status as a tool for evaluating animal robustness and resilience... Virginie Blanvillain, AB Vista What is "gut health" in animal nutrition? Pietro Celi, DSM Animal Nutrition Feed microbial quality supports animal health and performance goals Dr. Enrique Montiel, Anitox Sustaining laying performance in aged layers Denise Cardoso & Tim Goossens, Adisseo Improving the profitability of fattening pigs David Harrington, Advanced Ag Products LLC Intestinal health as driver for an efficient animal production system Arno Duchateau, Impextraco The race to immune competence how β-glucans can help Sanne van Beers, Selko Global

ISSUE FOCUS 22 FEED & ADDITIVE MAGAZINE October 2023 “Antibiotics are used in poultry production, mainly to improve gut health and performance. However, owing to growing concerns about the use of medically important antibiotics in livestock production and the associated challenges, poultry producers need effective holistic approaches including – management and nutrition – to support antibiotic stewardship. Currently, several dietary measures are taken to promote animal health in poultry production. Among these, an emerging group being included in holistic approaches that shows promise in improving gut health and host defense is postbiotics.” Poultry production plays a paramount role in ensuring food security for the current global population. Amidst the forecasted growth and potential shifts in demographics of global human population, the industry needs to produce more utilizing limited resources, without becoming over depending on antibiotics/chemicals to meet consumer needs. To achieve this goal, live production needs to be optimized with respect to animal performance, nutrition, and management to get the maximum use of resources without wastage. Nutrition/feed accounts for about 70% of poultry production costs. Because the animal gut is the primary organ which utilizes feed, it’s no wonder why gut health has been a major focus for the poultry industry over the past few decades. The future of the poultry industry depends largely on applying scientific knowledge and modern tools to support gut health and effective utilization of nutrients for sustainable production. IMPORTANCE OF GUT HEALTH A healthy gut is vital for the proper digestion and absorption of nutrients. In addition, the gut is critical for the animal to survive the stressors to which the body is exposed to throughout the gastrointestinal tract, and in maintaining homeostasis. Developmental stages and immune status of the bird, the quality of feed ingredients, changes in feeding phases, environmental stressors, management, and pathogens are some of the things that can disrupt homeostasis and affect gut health (Figure 1). Maintaining the integrity of gut tissue, nourishing a healthy microbiome, along with supporting an effective immune system are the main components to a healthy gut. From an animal production perspective, gut health is closely related to animal performance, welfare, and food safety. Antibiotics are used in poultry production, mainly to improve gut health and performance. However, owing to growing concerns about the use of medically important antibiotics in livestock production and the associated challenges, poultry producers need effective holistic approaches including – management and nutrition – to support antibiotic stewTHE ROLE OF POSTBIOTICS IN PROMOTING GUT HEALTH IN POULTRY Vivek Kuttappan, DVM/PhD Poultry Technology Lead – Postbiotics Cargill

ISSUE FOCUS FEED & ADDITIVE MAGAZINE October 2023 23 ardship. Currently, several dietary measures are taken to promote animal health in poultry production. Among these, an emerging group being included in holistic approaches that shows promise in improving gut health and host defense is postbiotics. WHAT ARE POSTBIOTICS? According to the International Scientific Association of Probiotics and Prebiotics (ISAPP), postbiotics are defined as, “a preparation of inanimate microorganisms and/or their components that confer a health benefit on the host”. Postbiotics are the output of a controlled fermentation which can contain various biologically active substances such as proteins, small peptides, oligosaccharides, vitamins, minerals, enzymes, and other undefined metabolites (Figure 2). Fermentation is a complex process where the output can vary based on the microorganisms, substrate, and environmental conditions involved in the process. So, an in depth understanding of the process is necessary to ensure the consistent composition and benefits from the product. The advantages of postbiotics over pre- and probiotics are that postbiotics have little dependency on further in vivo utilization of the product to have an effect on the host. Additionally, a postbiotic can maintain stability across industrial feed processing and long-term storage, making it more available to the animal. Moreover, a postbiotic product with nothing filtered out or isolated can be quite intricate, which makes it unique from single or few-compound feed additives. BENEFITS OF POSTBIOTIC IN POULTRY GUT HEALTH Several postbiotic products are used in different animals, including humans, because of their potential benefits such as anti-inflammatory, antibacterial, immunomodulatory, anticarcinogenic, antioxidant, antihypertensive, antiproliferative, and hypocholesterolemic properties. Among these postbiotic products, the Saccharomyces cerevisiae fermentation product (SCFP) is a major category used in livestock, including poultry. Scientific studies have reported that dietary inclusion of SCFP resulted in improved body weight, feed conversion ratio, and meat yield in broilers and turkeys, and improved egg weight, yolk weight, and hatchability in layers/breeders. One of the modes of action of SCFP is through modulating immunity, especially by Figure 1. Gut Health and Associated Factors Figure 2. ISAPP Postbiotic Consensus Definition