ISSUE FOCUS 26 FEED & ADDITIVE MAGAZINE July 2023 tive detection. However, this method is expensive in terms of initial capital outlay, analysis, and maintenance costs. The performance and proficiency of the Myco 9 Array was successfully validated in a third-party laboratory validation programme. This study involved third-party government and private laboratories, where different operators independently analysed 17 samples duplicated across all participating 3rd party laboratories. The Myco 9 Array showed high proficiency with 99% of the results falling within an acceptable Z-score range of -2|<Z<|+2 for all mycotoxins tested. Precision and accuracy range from 100% for DON, and 99% pass for AFs, ZEA, OTA and FBs. T-2 Toxins on the other hand recorded a 97% accuracy. The reproducibility co-efficiency of variation was between 16 - 20% meeting the set criterion of <40% and is, therefore, fit-for-purpose. Using a HorRat approach, the target reproducibility was met for DON, ZEA, T-2/HT-2 Toxins, FBs, OTA and AFB1,2, G1,2, assays. The study demonstrated that the Myco 9 Array results can be reliably reproduced for testing feed samples. There has been a consistent agreement between Myco 9 Array results and those of confirmatory methods for all nine mycotoxins including PAX and DAS. However, in this study samples were negative for the PAX and DAS assays, therefore, reproducibility data could not be computed for these two assays. The consistency of the data shows that the Myco 9 Array for the simultaneous detection of 9 mycotoxins in feed is fit-for-purpose. About Dr. Liberty Sibanda Dr. Liberty Sibanda, having completed his PhD in Pharmaceutical Sciences at Ghent University, has extensive knowledge of the diagnostics industry. An Applications Manager for Randox Food Diagnostics Ltd., Dr. Sibanda has over 20 years of experience developing and marketing mycotoxin test kits and has published numerous papers on the detection of mycotoxins in food and feed. Randox Food Diagnostics will be at the World Mycotoxin Forum in Belgium from the 9th – 11th October 2023, visit our booth or contact us to discuss our mycotoxin screening solutions further!
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