ISSUE FOCUS 46 FEED & ADDITIVE MAGAZINE May 2023 Wheat 1 2 34 5 6 78 9 101112 131415 161718 1 23 4 56 7 891011 EA concentration (µg/kg) 1205 442 EA concentration (µg/kg) ELISA LC-MS/MS 12 EA LC-MS/MS extra EA ELISA LC-MS/MS 12 EA LC-MS/MS extra EA 600 400 200 0 400 300 200 100 0 Barley SUMMARY AND CONCLUSIONS • Since 1st January 2022 there are European limits in effect for the presence of EAs in cereal products. • LC-MS/MS is the most common method for quantitatively measuring EAs. This method is precise, but also time-consuming and expensive. The industry needs a faster, cheaper method, so that contaminated batches can be identified earlier. • With the contribution of a consortium of companies led by WFSR, Randox Food Diagnostics has developed an ELISA that is sensitive enough to measure EA around the limit value. The ELISA has been validated for rye, barley, wheat, oats and spelt, and no false negatives were found for the field samples tested. • The ELISA requires only a simple sample preparation without the need for additional sample clean-up. • The ELISA seems to be sensitive to some EA that are not regulated, such as the clavine type EA. These EA usually occur in lower concentrations than the twelve regulated ones but can nevertheless lead to false suspect samples. ACKNOWLEDGEMENT *The research was carried out in project LWV19252, Dutch initiative for evaluation and quality assurance of fast methods in food safety testing, which was funded by TKI Agri&Food. The partners involved were: The Committee of Grain Traders, Nutrilab BV, Eurofins Labco, Randox Food Diagnostics and Wageningen Food Safety Research. For more information on Randox Ergot Alkaloids ELISA, visit their website here or contact them via email. This article has been translated from the Dutch original article which can be found here. Figure 3
RkJQdWJsaXNoZXIy MTUxNjkxNQ==