Feed & Additive Magazine Issue 25 February 2023

February 2023 Year: 3 - Issue: 25 Aurélien Piron, Lallemand Turning fiber into milk: The role of the rumen engine Gavin Boerboom, Selko Feed Additives Capturing the full value of trace mineral supplementation Marisabel Caballero, EW Nutrition Redesigning feed formulation during price hikes: What can nutritionists do? www.feedandadditive.com Increasing Digestibility & Feed Conversion Rate in Animals Managing Rising Feed Costs and Creating New Dietary Options with Alternative Feed Ingredients #Earthquake #Turkey #Syria

At the beginning of this week, we completed the final preparations of this issue and scheduled to publish. But on Monday morning (February 6th), we awoke to a great disaster. Turkey was shaken by two major earthquakes with a magnitude of 7.7 and 7.6, which occurred one after the other. These earthquakes, which were felt in a very wide area, caused great destruction and loss of life, especially in Turkey and Syria. While we were writing these lines, the loss of life in Turkey and Syria had approached ten thousand. Experts estimate these earthquakes as the most destructive earthquakes of our century. Currently, thousands of people are still trapped under the rubble. However, the spread of the disaster over a wide area (10 provinces in Turkey) and its devastating impact caused great damage to the transportation and communication infrastructure. Furthermore, severe winter conditions make rescue efforts extremely difficult. On the other hand, many countries around the world with one heart are making great efforts to support people in the earthquake zone in different ways. This is a very humane and morale boosting situation for the victims living in the earthquake-hit areas. Unfortunately, we were able to complete this issue of our magazine with a delay under the feeling of this disaster’s sadness. We hope that we have been able to prepare a useful issue for you once again. Hope to see you in the next issue... Publisher Muhammed Akatay [email protected] Responsible Manager Özlem Buyruk Akatay [email protected] Editor-in-Chief Derya Yıldız [email protected] Editors Bahadır Büyükkılıç Burak Varol [email protected] Design Alper Kandemir [email protected] Web Design Caner Cem Pulat [email protected] Proofreading Lisa Atakul [email protected] Circulation and Data Responsible Amine Şengün [email protected] Ataturk St. Metropol Istanbul Ste. Block C1, No:376 Atasehir, Istanbul/TURKEY Phone: +90 850 220 5064 E-Mail: [email protected] Web: www.feedandadditive.com Publication Type: Periodic (Monthly) EDITOR #earthquake #Turkey #Syria Derya YILDIZ © Three Plus Media | All rights reserved. Reproduction, in whole or in part, is prohibited without the written permission of the publisher. *DISCLAIMER: All views and ideas expressed in articles published in the magazine reflect the point of views of the author(s), not that of the Editor-in-Chief, Editors, Editorial Board or Publisher of Feed & Additive Magazine. The Editor-in-Chief, Editors, Editorial Board and Publisher assume no responsibility or liability in such cases. The publisher does not give any warranty and representation to the accuracy or completeness of the contents and data published in the magazine. All responsibility of the contents, articles and information published in the magazine belongs to the authors. The publisher and the authors cannot be held responsible for any damages resulting from the use of the whole or part of the content of the magazine. feedandadd i t i ve Dear Readers,

Minerals are an essential nutrient for production animals. It is very important to feed the most stable and bioavailable source of trace mineral, in order to optimise health, wellbeing and performance, as well as minimising environmental impact. THE BENEFITS OF INTELLIBOND MINERAL OPTIMISATION Increased stability Improved palatability Low solubility Increased bioavailability Improved fibre digestibility Smart minerals, Smart nutrition, Smart decision Learn more, visit www.selko.com

C O N T E N T S 18 ISSUE FOCUS 26 TURNING FIBER INTO MILK: THE ROLE OF THE RUMEN ENGINE Aurélien Piron, Lallemand Animal Nutrition - France 36 Feed efficiency in animal nutrition: HOW TO DO MORE WITH LESS? Caroline Chylinski & Catherine Ionescu, ADM Animal Nutrition 42 ADDRESSING EFFICIENCY: Late-finisher solution in the US shows value globally Kobe Lannoo, Agrimprove 20 NUTRITIONAL SOLUTION IMPROVING DIGESTIBILITY OF SOWS AND PIGLETS Aurélie Montagnon, Orffa Additives BV. 32 CAPTURING THE FULL VALUE OF TRACE MINERAL SUPPLEMENTATION Gavin Boerboom, Selko Feed Additives 38 "GUT HEALTH" IN ANIMALS, THE UBIQUITOUS TERM IN ANIMAL NUTRITION Jessika Van Leeuwen & Nicolas Anglade & Alfred Blanch, Hamlet Protein INCREASING DIGESTIBILITY & FEED CONVERSION RATE IN ANIMALS SPECIAL STORY 48 REDESIGNING FEED FORMULATION DURING PRICE HIKES: WHAT CAN NUTRITIONISTS DO? Marisabel Caballero, EW Nutrition 53 CONTROLLING COSTS THROUGH THE USE OF ALTERNATIVE INGREDIENTS IN POULTRY DIETS Diogo T. Ito, Hendrix Genetics Layers Managing Rising Feed Costs and Creating New Dietary Options with Alternative Feed Ingredients

C O N T E N T S ARTICLES PFAS found in organic eggs in Denmark 11 Dr. Eckel and Evonik become partners on phytogenics 13 IFF to offer two new solutions for poultry and swine in Canada 76 Protix appoints Lynn De Proft as new CFO 84 Bactocell authorized in EU as a technological additive 15 BioZyme launches new postbiotic solution for egg quality 79 PetDine’s facilities earn high safe quality food rating 72 Selko delivers two TECHTalks at IPPE for poultry producers 75 Easy Automation acquires IS Micro Ingredient System product line from GSI 83 NEWS REPORTS GLOBAL CATTLE FEED MARKET 64 SUCCESS STORY PLANNING, DESIGN AND IMPLEMENTATION IN ONE OF THE WORLD’S MOST DIFFICULT TIMES 45 61 INCREASING EGG PRODUCTIVITY AND SHELL QUALITY THROUGH ALKALINE THERAPY APPLICATION Asli Selvi, MSc, Intermak A.S. 58 CHOLINE TODAY, EUROS TOMORROW AND POSSIBLY FOR GENERATIONS TO COME Marcos Zenobi, MSc, Ph.D., Balchem

NEWS 6 FEED & ADDITIVE MAGAZINE February 2023 At the IPPE fair in Atlanta, BASF and Cargill announced their expanded cooperation, adding the United States (US) to their existing feed enzymes development and distribution agreement. Together, the two companies are committed to bringing innovative enzyme-based solutions to the market, generating distinctive value for animal feed customers. By combining the enzyme research and development strengths of BASF with Cargill’s know-how in application and broad market reach, BASF and Cargill expand their partnership to offer enzyme solutions in the US BASF and Cargill further expanded their partnership to offer high-performance enzyme solutions and to support animal wellbeing, farmer sustainability and productivity efforts in the United States. Nutreco and BiomEdit signed a partnership agreement with the aim of transforming feed additives through microbiome technology. Nutreco and BiomEdit announced a ground-breaking, long-term strategic research and commercial partnership to bring livestock producers innovative and truly novel feed additives developed through microbiome technology. The partnership aligns Nutreco Exploration (NutEx), Nutreco’s team tasked with developing proprietary ultra-specialty ingredients to promote its purpose of Feeding the Future, with animal health’s most advanced microbiome biotech company, BiomEdit. The collaboration covers the discovery, development and commercialisation of Biome-actives developed using BiomEdit’s advanced microbiome science and bioinformatics platform. These novel feed additives address the health and sustainability challenges of today’s animal producers, for aquaculture, poultry, swine and cattle. In recent years, studies into the gut microbiome have led to a basic understanding of their role in human and animal health and in how foods are digested in the gastrointestinal tract. Influencing the gut microbiome – the microbes – what they produce and their environment, for example through food supplements or medicines, can have a major impact on human health – and, similarly, can impact the health, well-being, and performance of animals. “Saying that microbiome science has created a buzz in the last decade is an understatement. However, practical applications of this scientific progress remain scarce. Instead of focusing on microbiome composition and how to affect it, at Nutreco, we believe that mining the microbiome to find microbial functions that will benefit the animal’s physiology will unlock real sustainable progress for the welfare, health, and productivity of farm animals,” explained Nutreco’s Chief Science Officer David Bravo. Read more>> Groundbreaking collaboration between Nutreco and BiomEdit

NEWS FEED & ADDITIVE MAGAZINE February 2023 7 the partners will form a joint innovation pipeline for animal protein producers. With the expanded geographical reach, BASF and Cargill aim to bring the voice and commercial insights of US protein producers to craft the next generation of enzymes jointly. Through this next stage of collaboration, BASF and Cargill strive to deliver solutions that address productivity, sustainability, and cost challenges for US customers. “We are delighted further to strengthen our relationship with our US customers and BASF,” said Mariano Berdegue, the North America regional managing director for Cargill’s animal nutrition business. “The collaboration will provide more animal feed customers with access to a wide range of high-performance enzyme solutions that we have seen reduce nutrient waste, improve feed efficiency, and sustainably promote animal performance.” In 2021, BASF and Cargill moved the relationship beyond pure distribution agreements into the joint development of new enzyme technologies and applications. This extended partnership builds upon the successful go-to-market collaboration between the companies across Argentina, Brazil, Mexico, Portugal, Spain, the Middle East, and Africa. As part of the partnership, BASF and Cargill will co-develop, produce, market, and sell customer-centric enzyme products and solutions. “With the expansion of our collaboration to the US, we continue to build our joint success story. We are proud to be on this journey with Cargill to increase further the value creation opportunities for animal feed customers,” added Gisele Santos Bin, Global Sales Director Feed Enzymes & Feed Performance Ingredients at BASF. Read more>> Evonik Animal Nutrition, sharing news about methionine production expansion at the International Production & Processing Expo (IPPE), will commission new methyl mercaptan production plant in Alabama in 2024. With the backward integration into methyl mercaptan, Evonik will be able to produce all necessary intermediates for its methionine production in Mobile itself – as is already the case in Evonik’s other global methionine hubs in Antwerp and Singapore. This investment serves the expanding global market for DL-methionine, underlining Evonik’s clear commitment to the industry. “This backward integration makes us more independent of fragile supply chains, guaranteeing that all-important supply security for our customers in the Americas, as well as globally,” said Gaetano Blanda, head of animal nutrition business line. “It also strengthens our leading cost position and makes it easier for us to boost future capacity for methionine to grow with our customers. We’re looking forward to discussing this and more at IPPE.” Methyl mercaptan is an important intermediate in the production of DL-methionine (MetAMINO®) which helps livestock farmers to feed their animals efficiently, healthily and sustainably. Read more>> Evonik to commission its methyl mercaptan production plant in 2024

NEWS 8 FEED & ADDITIVE MAGAZINE February 2023 Royal DSM, a global purpose-led science-based company, and Foundation Earth, an independent, non-profit organization issuing front-of-pack Eco Impact scores on food products, announce their agreement to cooperate on eco-labeling of food and beverages to empower consumers to make more sustainable food choices based on transparent and credible information. Within the new partnership DSM will undertake full Life Cycle Assessments (LCAs) for participating brands under the Foundation Earth method, enabling companies to access an aggregated eco impact score on their food and drink products, as well as an ecolabel that can be displayed on the front-of-pack. The scores delivered by Foundation Earth range from A+ to G and are re-certified yearly, making it possible for product owners to improve their production and grade over time. The agreement means that agrifood companies joining Foundation Earth can now access DSM’s Sustell™ service to measure and improve the environmental impacts of animal protein products (e.g. eggs, milk, fish and meat), based on accredited methodologies (e.g. EU PEF). Developed by DSM with Blonk Consultants, Sustell™ is a first-ofits-kind intelligent sustainability service that delivers accurate, simple, and actionable farm-level solutions to improve animal protein production’s environmental footprint and profitability. By catalyzing sustainable value chains and enabling environmental transparency and supply chain to differentiate, Sustell™ helps producers achieve their sustainability commitments. Read more>> The 6th International Poultry Meat Congress (UBEK), organized biennially by the Association of Poultry Meat Producers and Breeders, will be held in Titanic Deluxe Golf Belek Antalya between 1-5 March 2023. Many important scientists, experts and industry stakeholders from many countries of the world and Turkey are expected to participate in the organization, which is the only and largest congress to be held with international participation in the poultry industry. Turkey’s poultry meat industry, in the new world order; It is preparing to evaluate sustainable production and efficiency, self-sufficiency, local production, logistics service and technological developments at the 6th UBEK. Stating that the Poultry Meat Congress, which has been held every two years since 2011, could not be held for about 2 years due to the COVID-19 pandemic, BESD-BIR Chairman of the Board Naci Kaplan explains the importance of getting together again with the following words: “We are very happy to hold the largest and most important congress organized with international participation in Turkey for the sixth time. In addition, we are proud to organize our congress on the same date as the 100th anniversary of our Republic and the 30th anniversary of the Association of Poultry Meat Producers and Breeders (BESD-BIR). ” Read more>> International Poultry Meat Congress to bring scientists together in Antalya, Turkey DSM and Foundation Earth partner to promote food eco-labeling

NEWS FEED & ADDITIVE MAGAZINE February 2023 9 Symphiome™ Microbiome managed precisely When it comes to optimal gut health, the microbiome should function like an orchestra playing in perfect harmony. Conducting this orchestra is Symphiome™, a unique first-of-its-kind Precision Biotic. Symphiome™s unique mode of action increases metabolic functions intrinsic to the bird’s microbiome that detoxify unabsorbed amino acids and leaked host protein independent of the microbiota composition. Symphiome™ is the first-of-its-kind Precision Biotic that optimizes your birds’ resilience to enteric stress, aids in nutrient utilization, improves welfare, and reduces emissions. Symphiome™. Microbiome managed precisely If not us, who? If not now, when? WE MAKE IT POSSIBLE REDUCES EMISSIONS OPTIMIZES RESILIENCE TO ENTERIC STRESS AIDS NUTRIENT UTILIZATION IMPROVES WELFARE FEED | Follow us on: www.dsm.com/anh

NEWS 10 FEED & ADDITIVE MAGAZINE February 2023 Novus acquires Agrivida to develop new feed additives Volare to build industrial-scale facility for circular protein Novus International acquired biotech company Agrivida, with which it started a commercial partnership two years ago. Global animal health and nutrition company Novus International, Inc. announced its acquisition of biotech company Agrivida Inc. Through the purchase, Novus takes ownership of the proprietary Interius™ technology Agrivida developed to embed feed additives inside grain. This move by Novus comes two years after it began a commercial partnership with Agrivida to support the sale of the start-up’s flagship product, Grainzyme®. “Since December 2020, we’ve taken the time to understand and explore what Interius™ technology is capable of,” said Novus President and CEO Dan Meagher. “With this technology we believe we can revolutionize the feed additive industry through the expression of high-value, functional proteins inside grain, providing new products that are sustainable, both environmentally and operationally.” While the category of functional proteins includes enzymes, which Agrivida has already accomplished through Grainzyme®, the Novus Innovation team is expanding the product offerings to include antibodies embedded in grain. “Single-domain antibodies present an opportunity to naturally address the health challenges producers face in antibiotic-reduced and antibiotic-free production. These antibodies are a challenge to produce cost-effectively and deliver to the animal in a sustainable way,” said Novus Vice President and Chief Innovation Officer Al Zimmerman. “Interius™ technology addresses these challenges by removing costly fermentation and coating processes as well as offers the potential to stack different antibodies and/or enzymes in a single corn product solution for the animal. Traditionally these efforts would require blended products, which would mean more processing and cost. The Interius™ technology delivers this benefit in a single ingredient – corn.” Read more>> Deeptech company Volare, which produces insect-based protein, plans to build its first industrial-scale facility in Järvenpää, Finland. When opened, the facility will produce about 5,000 tons of environmentally friendly protein and lipids annually. Volare’s circular protein is used in fish feed and pet foods among others. Volare’s mission is to bring true circularity into food production. The new facility aims to support this mission with an annual 5,000 ton production capacity of insect-based protein and lipids. “We produce high-quality protein and lipids from the side streams of food production, and this has an extremely positive impact on the environment. With the new facility, we will be able to respond to the growing demand of climate-friendly protein in Europe,” said Tuure Parviainen, Volare’s CEO. Volare has applied its insect-based protein to dog food and bird food. The products are also suitable for instance feed applications for fish and other animals. In 2022, Volare’s circular protein became available to consumers through Alvar Pet’s dog treat and Leo & Wolf ’s seed bar for birds. The company believes that by changing the diets of animals, it is possible to make the entire food chain more sustainable. Volare has applied its insect-based protein to dog food and bird food. The products are also suitable for instance feed applications for fish and other animals. Read more>>

NEWS FEED & ADDITIVE MAGAZINE February 2023 11 Protenga expands smart insect farm with new funding One of the circular economy initiatives Protenga announced The Yield Lab Asia Pacific as its latest investor. “Looking to an eventful and successful 2022, we are delighted to welcome The Yield Lab Asia Pacific as our latest investor in Protenga, closing our current funding round for the first time. As we further expand Protenga’s operations, we look forward to working with The Yield Lab Asia Pacific team and accessing their extensive network of relevant industry experts,” the company said in a statement. The company will evaluate The Yield Lab Asia Pacific’s investment in the 2nd generation smart insect farm production platform. SEEDS Capital and JBI Innovations also attended the first close. “The Yield Lab has a fantastic Asia Pacific and Global reputation and presence, and the expertise to back it. We are strongly aligned with their mission to sustainably revolutionize agrifood systems thanks to our scalable renewal nutrition production platform” said Leo Wein, Founder and CEO of Protenga. Protenga’s technology production platform is developing fast, with current insect farming facilities having reached several key productivity and cost milestones this year. Based on these operating validations achieved in 2022, Protenga’s second generation Smart Insect Farm blueprint will see its first deployments together with strategic partners in 2023. Read more>> The environmental contaminant PFAS (Perfluoroalkyl and Polyfluoroalkyl Substances) was found in organic eggs from chicken farms from all over Denmark, whereas the content was low in eggs from free-range, barn and battery hens. This is shown by a study carried out by the DTU National Food Institute in collaboration with the Danish Veterinary and Food Administration. According to a statement from the institute, the substances are most likely transferred via the fishmeal used for feed. This relationship was found because uniform concentrations and compositions of PFAS compounds was found across large chicken flocks that consume organic feed. Especially children aged 4 to 9 years who eat more than 2.5 organic eggs per week are at risk of getting too much of the unwanted substance. The European Food Safety Authority, EFSA, has set the tolerable weekly intake of the sum of four specific PFAS (PFOA, PFNA, PFHxS and PFOS) at 4.4 nanograms per kg body weight per week. Among the children who eat many eggs (5-6 eggs per week), the intake is 10 nanograms per kg body weight per week. In addition, all citizens in Denmark are further exposed to PFAS from many other foods and sources, all of which contribute to the total intake. “When children are at risk of being exposed to more than twice as much PFAS solely from eggs as the amount that is the limit for a safe intake, the risk is noticeable. Especially when the intake for all age groups is close to the limit of what EFSA assesses as safe,” said Associate Professor Kit Granby from the DTU National Food Institute. Read more>> PFAS found in organic eggs in Denmark

NEWS 12 FEED & ADDITIVE MAGAZINE February 2023 Evonik, one of the world leaders in specialty chemicals, announced that it has invested €25 million in methionine intermediates production plant upgrade in Wesseling, Germany. The investment will safeguard long-term supply of MMP to European methionine production hub. MMP is integral in the production of MetAMINO® (DL-methionine) which is used in animal feed to improve the performance of livestock farming. The precursors of this essential amino acid have been produced in Wesseling for more than 50 years. Dr. Gaetano Blanda, head of Animal Nutrition business line, said: “With this investment, Evonik is strengthening the European methionine network to safeguard the long-term supply of MMP to our MetAMINO® production hub in Antwerp, Belgium. The move underscores our commitment to serving and expanding the global DL-methionine market and secures the best possible supply security for our customers.” The conversion and expansion of the plant in Wesseling has been made possible by the development of a new production process, that will allow to avoid the storage of chemical intermediates, such as acrolein, at the site. Commenting on the plant upgrade, Dr. Jan-Olaf Barth, head of the Essential Nutrition product line, said: “Evonik is known for its innovative approach and world-class technology, and in Wesseling we have now developed a process that increases efficiency and, with Responsible Care in mind, further enhances safety at the site.” Read more>> Evonik invests €25m in methionine intermediates production plant BioZyme® announced its new product AO-Biotics®, a line of fermentation products derived the company’s innovative fermentation of Aspergillus oryzae (AO) which promotes resilience. Thanks to AO-Biotics®‘s unique capacity to produce cell components and metabolites, it is ideal for producing innovative postbiotics. The first product addition to the AO-Biotics is Amaferm®. For the last 50-plus years, Amaferm has been the hallmark ingredient in BioZyme’s premier line of animal nutritional supplements and health products. It is a prebiotic research-proven to enhance digestibility. While research continues to expand on Amaferm, BioZyme is also on track to develop new products under the AO-Biotics brand. These will include both prebiotics and postbiotics. “We are very passionate about utilizing our expertise in AO fermentation to develop new products that address common challenges in animal agriculture globally,” said Lisa Norton, President and CEO. BioZyme, a member of the International Science Association for Probiotics and Prebiotics (ISAPP), has long manufactured and marketed prebiotics. However, inaugural sales of postbiotics are scheduled for the first quarter of 2023. Postbiotics are defined by ISAPP as “a preparation of inanimate microorganisms and/or their components that confers a health benefit on the host.” Read more>> BioZyme® launches new brand for fermentation products

NEWS FEED & ADDITIVE MAGAZINE February 2023 13 Available in Pellet, Crumble, Meal and Fine Powder forms. Dr. Eckel and Evonik become partners on phytogenics Dr. Eckel Animal Nutrition has entered into a partnership with Evonik, one of the world leaders in specialty chemicals, effective January 1, 2023. Evonik plans to launch a first product from the partnership – a phytogenic solution developed by Dr. Eckel – in Europe in the first quarter of 2023. Phytogenics are plant-based feed ingredients which play an important role in sustainable solutions for livestock farming. Dr. Eckel is one of the most experienced and innovative suppliers in the field of phytogenics. The value-orientated family business is one of the world’s foremost companies in the sector with a specific focus on animal welfare, developing products that make animal nutrition more resource-efficient, climate-friendly and healthy. “As a dynamic company with a high pace in product development, we are able to create innovations in a comparatively short period of time,” said Dr. Antje Eckel, founder of Dr. Eckel Animal Nutrition. “With a strong partner like Evonik, we can allow selected products to access a much wider market in a short time. We are therefore very happy that together with Evonik we can open up new target groups and customers.” Evonik Gut Health Solutions focus primarily on stabilizing the gut health of animals through probiotics such as Ecobiol®, Fecinor®, and GutCare®. The partnership with Dr. Eckel will allow Evonik to address further animal health issues with selected products that expand Evonik’s portfolio. Read more>>

NEWS 14 FEED & ADDITIVE MAGAZINE February 2023 Alltech has released its 2023 Alltech AgriFood Outlook recently, highlighting global feed production survey data. Despite significant macroeconomic challenges that affected the entire supply chain, global feed production remained steady in 2022 at 1.266 billion metric tons (BMT) in 2022, a decrease of less than one-half of one percent (0.42%) from 2021’s estimates. The annual survey, now in its 12th year, includes data from 142 countries and more than 28,000 feed mills. Europe bore the brunt of the impact, including significant disease challenges, severe weather and the impacts of the invasion of Ukraine. The global COVID-19 pandemic has had major impacts on the agri-food sector, contributing to supply chain challenges and accelerating the adoption of new technology and environmental sustainability practices. The top 10 feed-producing countries over the past year were China (260.739 million metric tons [MMT]), the U.S. (240.403 MMT), Brazil (81.948 MMT), India (43.360 MMT), Mexico (40.138 MMT), Russia (34.147 MMT), Spain (31.234 MMT), Vietnam (26.720 MMT), Argentina (25.736 MMT) and Germany (24.396 MMT). Together, the top 10 countries produced 64% of the world’s feed production, and half of the world’s global feed consumption is concentrated in four countries: China, the U.S., Brazil and India. Vietnam experienced a great recovery in terms of its feed tonnage in 2022, entering the top 10 ahead of Argentina and Germany and crowding out Turkey, which reported reduced feed tonnage. Russia overtook Spain, where there was a significant reduction in feed production. KEY OBSERVATIONS FROM THE SURVEY: • Feed production increased in several regions, including Latin America (1.6%), North America (0.88%) and Oceania (0.32%), while Europe decreased by 4.67%, Africa by 3.86% and the Asia-Pacific region also dropped 0.51%. • Globally, increases in feed tonnage were reported in the aquaculture, broiler, layer and pet food sectors, while decreases were reported in the beef, dairy and pig sectors. • Although it experienced a narrow reduction in feed production, China remains the largest feed-producing country in the world, followed by the United States and Brazil. Read more>> Alltech publishes global feed research Leading Netherlands-based shrimp importer Klaas Puul is teaming up with Nutreco-owned Skretting, the largest shrimp feed manufacturer in Ecuador, and Dutch sustainable feed ingredient suppliers Protix and Veramaris to supply supermarkets across Europe with more sustainable shrimp produced in Latin America. Albert Heijn, the largest supermarket chain in the Netherlands and part of global retailer Ahold Delhaize, is the first retailer to commit to the project in line with its strong GHG emissions targets and broader sustainability agenda. Farmed seafood is becoming an increasingly important source of protein as global demand, and the world’s population, grows. However, one significant constraint on the growth of the farmed seafood industry is its reliance on marine ingredients in aquaculture feed. For sustainable aquaculture supply growth, alternatives to marine ingredients need to be included in feed. Read more>> Leaders partner to supply sustainable shrimp to Europe

NEWS FEED & ADDITIVE MAGAZINE February 2023 15 Bactocell authorized in EU as a technological additive Lallemand Animal Nutrition, a company that produces and develops yeasts and bacteria, announced its unique lactic acid bacteria strain Pediococcus acidilactici CNCM I-4622 (Bactocell) has been authorized in the European Union as a technological feed additive in the functional group of acidity regulators and of hygiene condition enhancers. The authorisation is for use in all animal species (Regulation (EU) 2023/53). Bactocell was already recognized by the European Food Safety Authority (EFSA) for its safety and potential to reduce pH and limit growth of coliform bacteria in liquid feeds. Authorisation covers the application of the lactic bacteria in mash compound feeds and/or solid feed materials used for the preparation of liquid feeds at a minimum inclusion level of 1×109 (colony-forming units) CFU/kg feed. This is the first lactic acid bacteria to receive such an authorisation related to the hygiene of liquid feed. In Europe, liquid feed is particularly popular for swine in countries including Denmark and Sweden, where more than 60% of finishers — as well as the majority of sows — are fed liquid feed. Ensuring the sanitary quality of liquid feed and the feeding system itself is a key priority for producers. David Saornil, Product Manager for Swine Application at Lallemand Animal Nutrition, commented: “We are very pleased with this new authorisation that validates the benefits of Bactocell as a solution to improve liquid feed quality in sows, fattening pigs and piglets. Bactocell was already recognized for its probiotic benefits on gut health. We continue to document our benefits to producers and the industry as a whole.” Read more>>

NEWS 16 FEED & ADDITIVE MAGAZINE February 2023 De Heus inaugurates animal feed factory in Ivory Coast Purina announces new feed additives for sow and piglet De Heus Animal Nutrition, a fully-fledged nutritional supplier producing compound feed, premixes, and feed specialties, announced that it has inaugurated its first animal feed factory in Ivory Coast. De Heus Animal Nutrition opened a new greenfield animal feed factory situated in the new industrial zone of PK 24 in Attinguié with an initial capacity of 120.000 MT pa. The factory produces complete feeds and concentrates to ensure farmers in Ivory coast and surrounding export destinations have access to a full product portfolio- tailored to their specific conditions and goals. Koen de Heus, CEO of De Heus Animal Nutrition expressed his gratitude towards the honored guests for their support to make this factory a reality. “In Africa, typically, we establish an export market through our Koudijs division, who deliver high quality concentrates and pragmatic farming advice. Once we have enough insights and knowledge about the market we investigate if subsequent investment in a local, high quality modern factory can bring further growth and progress,” said Koen de Heus. He further explained how De Heus Animal Nutrition strives to improve the future and livelihood of farmers worldwide. Through bringing knowledge and production of customized, high quality animal feed, farmers all over the world are able to increase productivity, improve animal conditions, reduce environmental impact and bring progress for their families and their communities. Read more>> Purina Animal Nutrition, a wholly owned subsidiary of Land O’Lakes, announced two new products formulated to support sow performance and reproductive efficiency and piglet respiratory health and immune function during the post-weaning period - Purina® EnduraSow™ feed additive and Purina® EnduraPig™ feed additive, respectively. “Disease challenges can have long-lasting impacts on sow farm productivity,” said Erin Bryan, Ph.D., swine nutritionist with Purina Animal Nutrition. “For example, sick sows tend to eat less, and sows with low body condition at farrowing tend to lose additional weight during lactation. These sows are at risk of taking longer to breed back or requiring multiple services, which negatively impacts reproductive efficiency across the herd.” EnduraSow™ feed additive is a low-inclusion feed additive that helps optimize sow productivity during a health challenge, as measured by more pigs weaned per sow per year in commercial trials. The additive also supports optimal sow farm efficiency by reducing the number of days from weaning to first service. EnduraPig™ feed additive helps prepare pigs’ immune systems to address health challenges and supports pig growth performance throughout a respiratory challenge. This is especially important at weaning, when the immune protection from sow’s milk declines before the pig’s immune system fully matures. Read more>>

NEWS FEED & ADDITIVE MAGAZINE February 2023 17 INTRODUCING HIGHEST CHOLINE CONCENTRATION IN THE INDUSTRY 1, 3 Zenobi et al., J Dairy Sci. 101:1088 (2018) 2 Lima et al., The Veterinary Journal. 193:140-145 (2012). All trademarks are property of Balchem Corporation © 2021 Balchem Corporation. Balchem Italia Srl Via del Porto Snc 28040 Marano Ticino (NO) Italy Telephone +39 0321 9791 Fax +39 0321 979249 E-mail [email protected] Website BalchemANH.com To learn more, contact your local Balchem representative or distributor. Combining advanced core design with industry- leading encapsulation technology, Balchem delivers the most unique rumen-protected choline product on the market today. • Most researched base choline product in the industry • Highest payload while eliminating the need for a carrier • Durable and feed stable • Reduced carbon footprint • Non-GMO • European manufacturing Highest Choline Concentration in the Industry The ReaShure line is proven to deliver results throughout an animal’s life. • 2,10 kg of milk per day improvement, or 640,50 kg more milk over the full lactation.1 • Proven reduction in metabolic disorders including ketosis, displaced abomasum and subclinical milk fever.2 • In utero impact on the calf, leading to growth and health improvements.3 Advanced Technology, Proven Results


ISSUE FOCUS FEED & ADDITIVE MAGAZINE February 2023 19 Nutritional solution improving digestibility of sows and piglets Aurélie Montagnon, Orffa Additives BV. Turning fiber into milk: The role of the rumen engine Aurélien Piron, Lallemand Animal Nutrition - France Capturing the full value of trace mineral supplementation Gavin Boerboom, Selko Feed Additives Feed efficiency in animal nutrition: How to do more with less? Caroline Chylinski & Catherine Ionescu, ADM Animal Nutrition "Gut health" in animals, the ubiquitous term in animal nutrition Jessika Van Leeuwen & Nicolas Anglade & Alfred Blanch, Hamlet Protein Addressing efficiency: Late-finisher solution in the US shows value globally Kobe Lannoo, Agrimprove

ISSUE FOCUS 20 FEED & ADDITIVE MAGAZINE February 2023 "Overall, it can be stated that nutritional emulsifiers have a great potential to improve health and performance in the swine industry. Benefits are related to the emulsification activity and thereby improving fat digestibility and energy efficiency. Nutritional emulsifiers offer a valid strategy to reformulate sow diets and to support piglets in the post-weaning stress period.” Health and performance of animals depend greatly on their diets and digestive system. Regarding diets, the used raw materials and the needed dietary nutrients are to be considered. It is also important to take into account the use of the nutrients in the metabolism of animals, for maintenance and production. Moreover, in the current agricultural world, sustainability is of great importance to minimize the environmental impact. One of the principles of sustainability, defined by the FAO (Food and Agriculture Organization), is improvement of the efficiency in the use of resources. Nutrients’ digestibility and absorption are therefore key parameters to take into account when formulating a balanced ration for optimized performance, feed efficiency and sustainability. Several feed additives can support digestibility of key nutrients such as fat and energy. In this article, the functioning and benefits of using nutritional emulsifiers will be discussed. Nutritional emulsifiers are widely used in various species, including fattening pigs, but the application in sows and piglets is relatively new. New results in sows and piglets highlight the efficacy of nutritional emulsifiers. Especially during lactation, nutritional emulsifiers could be interesting due to the possible transfer of nutrients via the colostrum and milk, which benefit piglets in the long run. FAT METABOLISM AND NUTRITIONAL EMULSIFIERS Fats and oils are important energy sources in feed formulation. That’s why it is important to study the role of these ingredients in the metabolism of the animal, in order to optimize energy efficiency and animal production. In the digestive tract, fat globules are emulsified by bile salts which are excreted by the gall bladder. These bile salts form smaller fat droplets which will be further digested by lipases, enzymes catalyzing the hydrolysis of fats. The digestion of fats and oils will lead to the formation of free fatty acids and monoglycerides. Bile salts will again help with the emulsification of these fatty acids, creating micelles. Micelles are easier absorbed through the intestinal epithelial cell layer. Bile salts, so-called natural emulsifiers, are key components to improve fat digestibility as they help to create a higher amount of smaller droplets, increasing the surface of action and efficiency of the lipases. NUTRITIONAL SOLUTION IMPROVING DIGESTIBILITY OF SOWS AND PIGLETS Aurélie Montagnon Central Technical Manager Orffa Additives BV.

ISSUE FOCUS FEED & ADDITIVE MAGAZINE February 2023 21 However, several parameters are impacting fat digestion, such as the level of fat inclusion, fat characteristics (e.g. ratio unsaturated/saturated fatty acids), diet (e.g. particle size) and physiology of the animal (e.g. age). For instance, gut health is an important factor that can impact bile salts and reduce their efficiency. This can be the case in young piglets which have a non-optimal intestinal environment. At weaning, when transitioning from liquid to solid feed, their digestive system is not fully developed and adapted, so digestion and absorption of nutrients like fats can be limited. At this stage, the use of a nutritional emulsifier can complement the action of bile salts. A nutritional emulsifier improves the emulsification of the fat globules in the gastro-intestinal tract, further enhancing the digestibility of fat and efficiency of energy (Figure 1). DIET REFORMULATION AND DIGESTIBILITY By optimizing fat digestion, nutritional emulsifiers improve nutrients’ digestibility and energy efficiency. This is why they are already used in different animal species and different types of diets, with different fat/oil sources and levels. Use of nutritional emulsifiers can be explained by the flexibility in the types of inclusion in feed. Nutritional emulsifiers can be used on-top of the initial diet or in energy diluted diets. On-top application will result in improved feed efficiency and performance, due to the better use of energy for growth and milk production. To reduce feed cost, nutritional emulsifiers can also be used in energy diluted diets. Lower fat inclusion will be compensated by a higher digestibility and absorption of sources which are high in energy. Reformulation can be done by determining matrix values and the energy upgrade of the additive, and including the nutritional emulsifier in a classical feed formulation tool, as an ingredient. Both strategies were tested in a recent Brazilian trial, coordinated by the University of Sao Paulo. In total, 116 lactating sows were followed for 26 days, from 5 days before farrowing to weaning of their piglets. The sows were divided into three groups. A control group, on a basal diet. A second group on an energy reduced diet, which received the basal diet with a reduction of metabolizable energy by 50 kcal/kg feed and the inclusion of a nutritional emulsifier (Excential Energy Plus, Orffa Additives BV). The third group received the basal diet with the same nutritional emulsifier on-top. In total, the trial followed 1596 born piglets and 1451 weaned Figure 1. Fat digestion and mode of action of nutritional emulsifiers.

ISSUE FOCUS 22 FEED & ADDITIVE MAGAZINE February 2023 piglets, with a homogeneous distribution amongst the sows’ groups. It was clear that the addition of the nutritional emulsifier in lactation diets increased the piglet weight gain from birth till weaning for both inclusion strategies. Compared to the control group, piglet growth increased by 8.6% for the reduction of energy treatment and by 7.3% for the on-top treatment. Enhanced piglet growth can be explained by the numerical improvement of the milk production of the sows related to the addition of the nutritional emulsifier (Figure 2). Moreover, the feed conversion rate of the sows, measured by the kg of feed consumed divided by the kg of piglet weight gain, was numerically improved (Figure 3). No effects on sows’ feed intake and piglets’ mortality could be observed. Beneficial effects on health were observed by an improved colostrum Brix value. The Brix value was measured by a refractometer (a device measuring the refraction index) within 12 hours after delivery and is an estimation of the immunoglobulins G (IgG) in the colostrum. Higher IgG intake could result in an improved immune function of the suckling piglets. When classifying the proportion of sows having a poor (< 19.9), limit (20 – 24.9), adequate (25 – 29.9) and good (> 30) Brix value, a higher proportion of sows having an adequate Brix value in the nutritional emulsifier treatments was observed (53% with energy reduction treatment and 58% with ontop treatment, compared to 38% for the control). The combination of improved weight gain of the piglets, milk production and feed conversion rate of the sows showed that using a nutritional emulsifier, with on-top or reformulation feeding strategies, enables enhanced fat digestion and energy efficiency for optimal performance and health of sows and their piglets. SUPPORT DURING POST-WEANING STRESS As mentioned previously, the digestive system of piglets at weaning is still immature and limits the digestibility efficiency of nutrients. On top of that, weaned piglets suffer from post-weaning stress due to environmental and nutritional challenges. The weaning period can negatively impact their immune system, health status and overall production. In order to test the effect of nutritional emulsifiers to support Figure 2. Daily milk production of sows fed a control diet or a similar diet with a nutritional emulsifier, either on-top or with energy reduction. Figure 3. Feed conversion rate of sows fed a control diet or a similar diet with a nutritional emulsifier, either on-top or with energy reduction.

ISSUE FOCUS FEED & ADDITIVE MAGAZINE February 2023 23 weaned piglets and compensate for the poorly adapted digestion, a trial was performed in Japan following 47 piglets for 7 days after weaning (at 21 days of age). The piglets were randomly divided over 2 treatments: a basal diet and the basal diet supplemented on-top with a nutritional emulsifier (Excential Energy Plus, Orffa Additives BV). The addition of the nutritional emulsifier significantly improved both the body weight gain and the feed conversion rate, compared to the control group (Figure 4). Moreover, the feed intake was significantly higher. The nutritional emulsifier was able to support the piglets’ performance during the post-weaning stress period. The nutritional solution does not only show its benefits to digestibility and metabolism of piglets in relation to performance. Health is also a key factor to analyze, as it impacts greatly the resilience of piglets during the challenging period of weaning and their digestive system functioning. An Argentinian trial, following 30 sows during 22 days, from 90 to 112 days of gestation, brings this type of insight. The trial was coordinated by the National Scientific and Technical Research Council (CONICET). Half of the sows received a basal gestation diet, while the other half received the same diet with a nutritional emulsifier (Excential Energy Plus, Orffa Additives BV) on-top. After inducing labor at 114 days of gestation, the litter size was standardized to 13-14 piglets per sow. Remaining piglets, which were not selected, were adopted by other sows and not taking part in the trial. After weaning at 22 days of age, the 380 selected piglets were monitored for 15 days and randomly divided into four treatments: basal diet for the sows and piglets; basal diet for the sows and nutritional emulsifier on-top for the piglets; nutritional emulsifier on-top for the sows and basal diet for the piglets; and nutritional emulsifier on-top for the sows and piglets. The addition of the nutritional emulsifier to the diet of the piglets had several beneficial effects on the metabolism, intestinal morphology and activity of digestive enzymes. A significant increased Engineering your feed solutions www.orffa.com - Follow us on Find your local specialist at www.orffa.com EXCENTIAL ENERGY PLUS Saving energy, saving feed costs Excential Energy Plus: a 3rd generation nutritional emulsifier promoting energy and fat digestibilities “Excential Energy Plus gives swine farmers the opportunity to reduce feed costs, whilst getting the same growth performance and feed efficiency”

ISSUE FOCUS 24 FEED & ADDITIVE MAGAZINE February 2023 About Aurélie Montagnon Aurélie Montagnon is Central Technical Manager at Orffa Additives BV. Aurélie graduated from ISARA, France in 2020, with an engineering master’s degree in Agriculture, environment and resources management, with specialty in breeding, nutrition, environment and health. concentration of citrulline in the plasma was observed in piglets receiving the nutritional emulsifier in their diet (P < 0.05). Citrulline is a marker of the metabolic activity and functional mass of enterocytes in the intestine. The observed higher level showed the effect of the nutritional emulsifier to support piglets’ metabolism. Next to plasma components, a significant increased maltase activity was measured (Figure 5). In the digestive tract, maltase is an enzyme catalyzing the hydrolysis of maltose into glucose. Moreover, the addition of the nutritional emulsifier improved significantly the villus height, villi/crypt ratio and intestinal absorption surface in the jejunum and the ileum (P < 0.05). The treatment of the sows did not have any influence on the measured parameters. Combined, these results show the potential of a nutritional emulsifier to improve digestibility, absorption and intestinal health. The metabolism and digestive system of the piglet are better adapted to their new diet. This could lead, in the long run, to improved growth performance and feed efficiency of the pigs. NUTRITIONAL EMULSIFIERS FOR A BETTER FEED EFFICIENCY Overall, it can be stated that nutritional emulsifiers have a great potential to improve health and performance in the swine industry. Benefits are related to the emulsification activity and thereby improving fat digestibility and energy efficiency. Nutritional emulsifiers offer a valid strategy to reformulate sow diets and to support piglets in the post-weaning stress period. Focusing on feed efficiency is of vital importance nowadays in order to maximize the output of animals while keeping control of feed costs. In this way, margin and return on investment can be optimized. This is why different tools to improve digestibility are taken into account. Next to feed additives, choosing optimal ingredients with optimal digestibility coefficients and optimizing feed processing methods can benefit the animal industry. Nutritional emulsifiers, in combination with other strategies, can support the better use of raw materials and minimize the impact on the environment. Figure 4. Percentage change to the control with the nutritional emulsifier treatment on the body weight gain, feed intake and feed conversion rate in weaned piglets (P < 0.05). Figure 5. Intestinal maltase activity, when feeding the basal diet or the nutritional emulsifier to weaned piglets (P < 0.05).


ISSUE FOCUS 26 FEED & ADDITIVE MAGAZINE February 2023 Ruminants have the unique ability to release energy from plant fiber thanks to the fermentative activity of the rumen. The fiber components, and the subsequent production of volatile fatty acids (VFAs), provide the majority of the energy for the ruminant. Better understanding of how fiber is degraded in the rumen helps identify the levers that can help make sure fiber is no longer an untapped energy source. TURNING FIBER INTO MILK Fiber is slowly degraded in the rumen due to its physical structure and the lignin cross-linking that holds the fiber intact. Fiber degradation in the rumen is influenced by: • The anatomy of the fiber (pectin, hemicellulose and cellulose concentration), which is related to species • Lignin content and structure (maturity, species, stressors, etc.) • Particle size • Passage rate (highly influenced by particle size, uNDF, DMI) and its impact on rumen dynamics • Cud-chewing and ruminal contractions • Rumen microbial population (microbiome) influenced by several factors (diet, environment) The ruminant reduces the particle size of the forage through the eating process via initial mastication (chewing). These particles are swallowed and float to the top of the rumen mass. The particles are then regurgitated and rechewed (cud-chewing) to increase moisture content and surface area of the material as well as to abrade the fibrous portion. After initial mechanical abrasion of fiber through mastication, the feed is then exposed to the diverse population of microorganisms in the rumen. The first step in fiber degradation is the colonization in the rumen of the particle of fiber by fungi and bacteria. Fibrolytic microbiota are very sensitive to oxygen. Strict anaerobic conditions (measured though reTURNING FIBER INTO MILK: THE ROLE OF THE RUMEN ENGINE Aurélien Piron Ruminant Technical Manager Lallemand Animal Nutrition - France Key role of the rumen in digestion • A huge fermenter (up to 200 L) with unique digestive capacity • Over 100 billion microbes/mL of rumen fluid • A complex ecosystem: anaerobic bacteria, ciliate protozoa, fungi, archaea • Up top 70% of the feed is digested in the rumen