Feed & Additive Magazine Issue 23 December 2022

December 2022 Year: 2 - Issue: 23 Dr Maygane Ronsmans, Beneo The natural way to support a pet’s gut health Susanne Wiegel, Nutreco Cellular farming or cultured meat: What does it mean for livestock? Lien Vande Maele, Orffa Betaine as nutritional strategy to improve meat quality www.feedandadditive.com Cultured Meat and its Impact on Livestock Industry New Trends in Pet Foods and Pet Food Ingredients

Animals have been an invaluable part of human life for centuries. Throughout history, they have met and continue to meet our needs in many areas from nutrition to transportation, from protection to health, from trade to emotional support. It would not be an exaggeration to say that the sustainability of human life is possible with the existence of animals. Because animals have an important role in ensuring the continuity of the ecosystem that provides us the opportunity to live. Defending the life rights and well-being of living things that have such vital roles for human beings is now much more than a personal choice or a conscientious approach…This is a serious responsibility and everyone should share this responsibility too. Fortunately, awareness of this issue is increasing today. There are more and more people following closely the rights to life and welfare of wild animals, farm animals and pets. Legislation in this regard is also increasing rapidly. When the issue of the right to life and welfare of animals is mentioned - probably because we share a one-to-one life - we see that there is a special focus on pets. Especially with the Covid-19 pandemic, the interest of people for pets has intensified when they spend a long time indoors. Our little friends at home managed to be our biggest supporters in coping with the feeling of loneliness during this difficult term. With the pandemic, people's spending more time with their pets also mediated the rise of a new trend: Humanization… People now see their pets as members of their families, so they focus more on their general needs, nutrition and health. This trend is also driving an entire pet nutrition and health industry to reconsider their solutions. So, we cover “New Trends in Pet Foods and Pet Food Ingredients” in this last issue of 2022 and we discussed the changing new approaches in pet food with valuable industry representatives. In addition, new alternative protein sources such as "cultured meat", which are among the topics that have attracted attention in recent years, and "cellular agriculture" are among the topics we have discussed in this issue. I believe that the interview we did with Nutreco, one of the important representatives of the animal nutrition industry, on its investments in cellular agriculture and alternative protein sources, is remarkable in understanding the future of this field and its meaning for traditional livestock. Hope you will enjoy reading this issue… See you in 2023... Publisher Muhammed Akatay [email protected] Responsible Manager Özlem Buyruk Akatay [email protected] Editor-in-Chief Derya Yıldız [email protected] Editors Bahadır Büyükkılıç Burak Varol [email protected] Design Alper Kandemir [email protected] Web Design Caner Cem Pulat [email protected] Proofreading Lisa Atakul [email protected] Circulation and Data Responsible Amine Şengün [email protected] Ataturk St. Metropol Istanbul Ste. Block C1, No:376 Atasehir, Istanbul/TURKEY Phone: +90 850 220 5064 E-Mail: [email protected] Web: www.feedandadditive.com Publication Type: Periodic (Monthly) EDITOR HUMANIZATION TRENDS IN PETS Derya YILDIZ © Three Plus Media | All rights reserved. Reproduction, in whole or in part, is prohibited without the written permission of the publisher. *DISCLAIMER: All views and ideas expressed in articles published in the magazine reflect the point of views of the author(s), not that of the Editor-in-Chief, Editors, Editorial Board or Publisher of Feed & Additive Magazine. The Editor-in-Chief, Editors, Editorial Board and Publisher assume no responsibility or liability in such cases. The publisher does not give any warranty and representation to the accuracy or completeness of the contents and data published in the magazine. All responsibility of the contents, articles and information published in the magazine belongs to the authors. The publisher and the authors cannot be held responsible for any damages resulting from the use of the whole or part of the content of the magazine. feedandadd i t i ve

Perform efficiently with confidence Poultry feed can amount to 70% of your overall production costs. Efficient nutrient utilization in your flock ensures healthy development, boosts productivity, and improves performance. By improving efficiency and sustainability, we at DSM help drive your flock’s performance and your profitability with confidence. If not us, who? If not now, when? WE MAKE IT POSSIBLE Follow us on: www.dsm.com/anh

18 ISSUE FOCUS 24 ADM ACCELERATES PLANET-FRIENDLY PET FOOD INNOVATIONS Jorge Martínez, Pet Nutrition, ADM 32 NOVEL AND GREEN HUMAN-GRADE PET FOOD FORMULATION LANDSCAPE Behnaz Shakersain, PhD, AstaReal Sweden 42 WHY IS IT IMPORTANT TO GIVE YOUR DOG WEIGHT CONTROL? Loi Lee, Lachance Group 40 FIBER INNOVATIONS – TIME TO SHINE Dr. Astrid Bosse, JRS Petfood & Aquafeed 20 TRENDS IN PETFOOD AND ROAD TO REGULATORY APPROVAL WITHIN THE EU Dawn Botha, Pen & Tec Consulting 28 IMPROVING PET FOOD DIGESTIBILITY WITH YUCCA SCHIDIGERA Emily Dickson, Alltech Inc 36 THE NATURAL WAY TO SUPPORT A PET’S GUT HEALTH Dr Maygane Ronsmans, BENEO NEW TRENDS IN PET FOODS AND PET FOOD INGREDIENTS 44 CELLULAR AGRICULTURE OR CULTURED MEAT: WHAT DOES IT MEAN FOR LIVESTOCK? Susanne Wiegel, NuFrontiers, Nutreco 48 CELLULAR AGRICULTURE: A POWERFUL SUPPLEMENT FOR THE FUTURE Robert E. Jones, Mosa Meat SPECIAL STORY Cultured Meat and its Impact on Livestock Industry

ARTICLES Skretting Vietnam inaugurates Lotus II fish feed factory 9 Bühler unveils Granulex® 5 series, the next generation hammer mill platform 11 Antimicrobial resistance: Now is the time for collective action 82 Darling Ingredients to acquire Polish rendering company Miropasz 88 Cargill acquires Owensboro Grain Company 12 Cargill announces its two senior appointments 84 Wynnstay acquires feed supplier Tamar Milling 90 Alltech launches Egalis™ range of silage inoculants 14 Nutreco announces acquisition of Mosegården’s nutritional activities 78 Unibio's shrimp trial shows 75% improved survival rate 86 NEWS REPORTS GLOBAL INSECT PROTEIN MARKET 72 58 THE BEAUTY OF THE BLEND - HOW ORGANIC ACIDS SUPPORT FEED SAFETY AND SHELF LIFE Agata Kozuchowicz, Selko Feed Additives 62 HOMEOSTASIS – THE GOLDEN PATH TO EFFICIENT POULTRY PRODUCTION Dr. Ruth Raspoet, Phileo by Lesaffre 68 INTELLIGENCE IN OPEN SEA AQUACULTURE - AI ENABLING SEAFOOD SUSTAINABILITY R. Dinesh, Mandapam Centre for Sustainable Aquaculture (MCeSA), TNJFU 50 BETAINE AS NUTRITIONAL STRATEGY TO IMPROVE MEAT QUALITY Lien Vande Maele, Orffa Additives BV. 54 MAXIMIZING BROILER PRODUCTION GROSS INCOME Christy de Wildt, Adisseo NL B.V.

NEWS 6 FEED & ADDITIVE MAGAZINE December 2022 Royal DSM, a global purpose-led science-based company active in Health, Nutrition and Bioscience, began construction of a new manufacturing facility at its site in Dalry, Scotland. The plant will significantly increase global production of Bovaer®, DSM's unique methane-reducing feed additive for cattle. In an official groundbreaking ceremony attended by the First Minister of Scotland, Nicola Sturgeon, and Dimitri de Vreeze, Co-CEO of DSM, the company marked the next step in its plan to use Bovaer® to reduce global methane emissions and contribute to worldwide methane reduction targets. DSM announced that planning had started during the UN Climate Change Conference last year in Glasgow. Construction officially began today on a facility that will help DSM scale-up production of Bovaer® in the coming years and marks another milestone in the company's ambition to bring its methane-reducing feed additive to the market worldwide. This development of DSM's Dalry site has been supported by the Scottish Government through Scottish Enterprise, the national agency supporting economic development and innovation. Reducing methane emissions is crucial for keeping DSM starts construction of production facility for Bovaer® DSM announced that it has started construction of large scale production facility for its novel methane-reducing feed additive for ruminants, Bovaer® in Scotland. Calysseo, a joint venture between world-leading alternative protein company Calysta and worldwide animal nutrition leader Adisseo, has announced it intends to build an alternative protein facility in Al Jubail in the Kingdom of Saudi Arabia in partnership with Food Caravan. Advanced discussions and detailed planning are underway between the partners, who have formalised their intent to move forward with a development in the country. The partnership is aiming to build a 100,000 tonne-capacity fermenter in the Kingdom of Saudi Arabia to produce Calysta’s novel protein ingredients, which require no animal or plant ingredients to produce. It is hoped the facility could become operational by the end of 2026, pending necessary construction and approvals. Confirmation over the intent to build comes after Calysseo announced it has successfully switched on its maiden 20,000 tonne fermenter in China, which will produce FeedKind® Aqua protein for the aquaculture market. The KSA facility would be five times the size of the current Chinese fermenter. “Our mission is to help the world meet one of its most pressing goals – feeding a global population that’s set to hit 10 billion by 2050. To do that, we need more sustainable protein, and to produce protein in places we are not currently able to. That’s what’s so exciting about the possibility of a Calysseo facility in KSA,” said Alan Shaw, CEO, President, and co-founder of Calysta. “This would be our second industrial-scale plant and an important step on delivering enough sustainable protein to meet growing and pressing global demand. Our patented fermentation technology is proven to produce a high-quality protein ingredient and thanks to the efficiencies of using a naturally occurring bacteria process, we are ready to rapidly scale up and meet that demand.” Read more>> Calysta and Adisseo partnership plans to build alternative protein facility in Saudi Arabia

NEWS FEED & ADDITIVE MAGAZINE December 2022 7 Alltech, one of the world’s largest producers of organic minerals for animal nutrition, opened its first organic mineral production facility in Vietnam. Alltech, a global leader in the agriculture industry, opened its newest Bioplex® plant, the first organic mineral production facility in Vietnam. The state-of-the-art manufacturing plant is Alltech’s eighth mineral production facility worldwide. The plant’s production capacity is 7,000 metric tons per year, and it has the potential to create 100 new jobs, while being conveniently located to serve local customers and support import/ export activity. Alltech is one of the world’s largest producers of organic minerals for animal nutrition. The company has committed significant resources into organic trace mineral production, quality control and research, as trace mineral nutrition is essential to animal health. Alltech’s Bioplex® range of minerals are supported by more than 21 years of research and offer higher bioavailability to animals than inorganic minerals. The minerals are readily absorbed, stored and utilized by the animal, supporting overall health, immune status and reproductive function. Furthermore, because Alltech’s organic minerals are better utilized by animals, producers are able to achieve more with less. Mineral excretion from animals through manure is also reduced, resulting in less environmental impact. “Our organic mineral program reflects our focus on sustainability in all aspects, from the health of the animal and the nutrition of the meat, milk and eggs produced to the economic well-being of the producer and the impact we have on our planet’s land and water,” said Dr. Mark Lyons, president and CEO of Alltech. “Our investment in enhancing our mineral production in Asia reflects our confidence in the market’s continued growth and our alignment with our customers’ commitment to better nutrition from farm to market.” Read more>> Alltech opens Vietnam’s first organic mineral production facility warming below the 1.5°C target outlined in the Paris Agreement. Over 100 countries have signed a Global Methane Pledge, agreeing to reduce global methane emissions by at least 30% by 2030. The addition of new signatories in recent months, such as Australia in October 2022, underscores growing recognition of the need for urgent action. Ruminants (mainly cows) emit around 20% of all methane gasses globally, so reducing emissions in ruminant herds will play a vital role in meeting global climate ambitions. Dimitri de Vreeze, Co-CEO of DSM, commented: "After many years of hard work and dedication, it's a wonderful feeling to attend the groundbreaking ceremony for such a large-scale production facility. This is a historic moment for DSM: We are continuing to deliver on our 2030 methane pledge, one of our food systems commitments. I wish the construction team every success as they develop this plant in the coming years." Read more>>

NEWS 8 FEED & ADDITIVE MAGAZINE December 2022 Research insight into mycotoxin mitigation in dairy cows Nutreco invests in cellular agriculture by taking a minority share in Roslin Tech Scientists renowned for their research on mycotoxin risk management in livestock shared insights on how mycotoxins affect the bottom line of dairy cow operations during the Selko Feed Additives global webinar “Multipronged Approach to Mycotoxin Risk Management in Dairy Cows,” on 27 October 2022. Prof. Antonio Gallo, Animal Nutrition Department at Università Cattolica del Sacro Cuore, Italy and Dr. Swamy Haladi, Global Programme Manager, Mycotoxin Risk Management at Selko, shared how dairy cows are exposed to higher number of mycotoxins as compared to monogastric animals, the challenges faced in diagnosing mycotoxicosis, the economic impact of mycotoxins and the latest research on the practical strategies for mitigating mycotoxin risk in dairy cows. Both geopolitical and climate factors are elevating mycotoxin risk for livestock production. Beyond a reduced grain supply resulting from the Russia-Ukraine conflict, widespread droughts have lowered grain production in many regions. Lower grain supplies have led to concerns about moulds and grain quality, triggering import alerts. Ruminants are often thought to be less susceptible to mycotoxins than monogastric animals. The rationale is that the rumen helps break down contaminants in the rumen. While the rumen protects against some contaminants, it does not defend against all mycotoxins. For some mycotoxins, the mycotoxin degradation in the rumen can lead to more toxic metabolites than the parent mycotoxin. The complex dairy cow diet presents more opportunity for contamination with multiple types of mycotoxins. Read more>> Nutreco announced that it has taken a minority share in Scotland-based Roslin Technologies Limited in the company’s recently closed Series A funding round. The £11 million funding round is intended to fuel Roslin Tech’s next wave of growth in its mission to become the leading provider of animal cell lines to the emerging cultivated meat sector. This latest investment gives Nutreco a stake in another link of the developing cultivated protein supply chain and enables the company to make further progress towards its purpose of Feeding the Future. Nutreco expects the animal protein markets to continue to grow and to offer exciting prospects and remains fully committed to supporting livestock and aquaculture customers with ingredients and technologies. However, to sustainably feed an ever-growing population, the world will need more solutions than animal protein – and cultured meat is a great alternative. “Insights gained from this partnership could provide an opportunity for Nutreco to supply ingredients to the cultivated protein industry and accelerate our commitment to our purpose of Feeding the Future,” said Nutreco CEO Fulco van Lede. “It offers us a pole position in combining genetics with feed leadership – amplifying our commitment to innovation, quality and sustainability.” Read more>>

NEWS FEED & ADDITIVE MAGAZINE December 2022 9 Specialty Feed Additives to optimise animal performance and customer value Learn more, visit www.selko.com YOUR SELKO BENEFITS Unique products backed by sound science Expertise to help customers succeed Manufactured at the highest quality standards Skretting’s second aquafeed factory in Vietnam opened in Long An Province on 18 November. The investment aims to promote sustainable growth – to better serve and bring mutual benefits to customers in the region. Long An Province was strategically chosen due to its convenient proximity to the Mekong Delta, which is regarded as the cradle of Vietnam’s seafood industry. The facility is also a key element of Skretting’s strategy to develop further in Asia. Supported by its two factories in Long An Province, Skretting Vietnam specialises in the production of high-quality feeds for the domestic market as well as for many major aquaculture operations across South Asia. The new factory is Vietnam's most modern fish feed factory to date. With a total investment of $24 million, the factory boasts two modern production lines with an annual production capacity of 100,000 tons. It is equipped with a highly automated management system and features two separate production lines that can manufacture different products without the risk of cross-contamination. Additionally, an ultra-fine grinding system increases the nutritional uniformity of each pellet, which in turn improves the ability of fish to absorb nutrients. Read more>> Skretting Vietnam inaugurates Lotus II fish feed factory Skretting announced the opening of its second aquafeed factory in Vietnam as a key element of its strategy to develop further in Asia.

NEWS 10 FEED & ADDITIVE MAGAZINE December 2022 Elanco Animal Health Incorporated, one of the global leaders in animal health, and Tonisity International Limited, an animal health and nutrition company based in Ireland, have entered into a Distribution and Promotion Agreement in respect of Tonisity’s novel, gut enhancing porcine products, Tonisity Px™ and Tonisity PxW™. Under the terms of the Distribution and Promotion Agreement, Elanco will act as exclusive distributor of Tonisity’s Swine Products in Brazil, Belgium, Denmark, France, Germany, Norway, Portugal, Spain, Sweden, and The Netherlands. As exclusive distributor, Elanco will be responsible for marketing, promotion and distribution and sale of the Swine Products in these markets. The specific transition timelines to a distribution via Elanco in early 2023 are currently being elaborated. Both Elanco and Tonisity have agreed to explore collaboration opportunities in other markets, when appropriate. The addition of Tonisity’s Swine Products to Elanco’s portfolio fits perfectly with Elanco’s strategy of promoting the intestinal health of animals, improving their overall health and leading to a more efficient production, providing its customers an innovative product portfolio. Read more>> Elanco and Tonisity form strategic partnership Kemin Industries expands encapsulation capabilities with two new facilities Kemin Industries has increased its production of encapsulation ingredients at manufacturing site in Italy with two new facilities and added pilot plants for product development. Kemin Industries, a global ingredient manufacturer that strives to sustainably transform the quality of life every day for 80 percent of the world with its products and services, has expanded its encapsulation production site in Cavriago, Italy, with two new facilities. To inaugurate the new manufacturing plants, Kemin Animal Nutrition and Health – EMENA (Europe, Middle East, North Africa) hosted a global customer event, delivering exclusive insight into the next generation of ruminant nutrition. More than 10 years ago, Kemin initiated a worldwide strategy to elevate its role as a major player in the ruminant industry. The company has invested in its ruminant solutions by hiring new experts, focusing on innovation and strengthening its expertise in encapsulation technology. In 2019, this led Kemin Animal Nutrition and Health – EMENA to launch two new products: KESSENT® and CholiGEM™. The introduction of KESSENT made Kemin the only company to provide Methionine and Lysine in a reliable way. KESSENT, along with CholiGEM, the choline-chloride ingredient to improve animal health, rounded out Kemin’s Ruminant Essentialities product portfolio, offering a fullrange of solutions for livestock health and nutrition needs. The expansion of Kemin’s encapsulation facilities is another step toward optimally serving customers worldwide. Read more>>

NEWS FEED & ADDITIVE MAGAZINE December 2022 11 ADM launches new sweetening feed solutions for piglets ADM has launched two products under its SUCRAM series, designed to enhance the palatability of feeds for young animals, especially weaned pigs. ADM, a global leader in human and animal nutrition, announced the launch of two products under its SUCRAM range, an in-feed sweetening solution for livestock species. Developed by ADM’s global feed additives business, Pancosma, SUCRAM M’I Sweet and SUCRAM Specifeek are sweeteners designed to improve the palatability of feed for young animals – particularly weanling pigs. Palatants and sweeteners are useful nutrition solutions to improve feed intake and enhance efficiencies for producers while also supporting animal health and well-being. “Significant diversity exists between the sweet taste receptors among different species, impacting the perception of sweetness,” said Fabio Catunda, ADM Global Swine Commercial Director. “What is sweet for one species may not be perceived as sweet in another species due to these inter-species differences. This is critical to understand when developing effective sweetening products such as SUCRAM.” In-feed sweeteners have demonstrated to be an effective attractant to encourage feed intake at the weaning stage, as piglets make the transition from liquid to solid feed. Read more>> Bühler unveils Granulex® 5 series, the next generation hammer mill platform Bühler announced the launch of the Granulex® 5 series. After a decade of research, the new hammer mill series packs in many small innovations that together add up to a big boost in performance. Its groundbreaking modular system offers significant energy savings and flexibility while maintaining the highest product quality and safety standards. Grinding is one of the most energy-intensive process steps in many food and feed plants. In today’s competitive markets, rising energy costs are a serious issue. A big challenge faced by businesses that rely on grinding is, therefore, how to make more with less. Greater energy efficiency is one of the prime benefits of the new Granulex® 5 series, with 10% reduction in energy consumption per ton, and in some cases the reduction can be as much as 30%. The new hammer mill also has the highest throughput ranges, improved granulation profile, and an operator-friendly design. Capacity is increased by 10% and the time required for screen changeovers is reduced by 50%. The new solution can also be connected to Bühler Insights to enable 24/7/365 data-based decision making. The hammer mill is among the most widely used grinding solution in industrial food and feed processing plants, valued for its flexibility and adaptability. “It is not easy to revolutionize an old machine category,” says Dean Ekkaia, Product Management Director Value Nutrition at Bühler Group. Read more>>

NEWS 12 FEED & ADDITIVE MAGAZINE December 2022 Protix, one of the leading companies in insect ingredients for feed and food, has released impressive comparative sustainability figures for its products following a Life Cycle Assessment (LCA) conducted by the Deutsches Institut für Lebensmitteltechnik (DIL e.V.). The analysis shows outstanding scores for four insect-based ingredients from Protix, which food, feed and fertilizer manufacturers can use to reduce their ecological footprint, while addressing demand for more sustainable nutrition. Highlights of the LCA, a commonly accepted way of evaluating the effects that a product has on the environment over the entire period of its life, include: - The CO2 footprint and impact on global warming is up to 24 times lower with insect-based PureeX® (at 0.439 CO2 equivalent per kilogram product) than with poultry meat (10.33 kg CO2 equivalent) which is commonly used in pet food as a high moisture protein source - ProteinX® insect meal (at 1.149 kg CO2 equivalent) has almost a seven-fold lower CO2 footprint than the soy protein concentrate (at 7.5 kg CO2 equivalent) often found in livestock and aqua feed - Flytilizer®, Protix's insect-based fertilizer, shows only 0.02 kg CO2 equivalent per kilogram of product - Each kilogram of ProteinX® reduces water consumption by 330 litres (190 litres vs 520 litres for soy protein concentrate) - Replacing coconut oil with LipidX® in pet food and livestock feed returns over 12m2 of arable land to nature for each kilogram of fats. LipidX® uses 0.898 m2 of land compared with coconut oil at 12.98 m2. Read more>> Cargill and Owensboro Grain Company, a fifth-generation family-owned soybean processing facility and refinery located in Owensboro, Ky., announced that they have entered into a definitive agreement where Cargill will add Owensboro Grain Company (OGC) to its North American agricultural supply chain business. Owensboro Grain Company was founded in 1906 as a small grain merchant and today operates a fully integrated soy processing facility, producing soy products, including protein meal and hull pellets for animal feeds, crude and degummed oil, lecithin, various blends of refined vegetable oil for human consumption, biodiesel, glycerin and industrial waxes. The addition of Owensboro Grain Company enhances Cargill's efforts to modernize and increase capacity across its North American oilseeds network to support growing demand for oilseeds driven by food, feed and renewable fuel markets. "We are pleased to welcome Owensboro Grain Company into our Cargill family," said Leonardo Aguiar, president of Cargill's North American agricultural supply chain. "Our two companies have tremendous operational histories, similar heritages as grain merchants, and values, including an unwavering commitment to prioritizing people - making this a tremendous fit. Additionally, this is a significant milestone in Cargill's journey to create a connected and modernized grain experience for our customers." Read more>> Cargill acquires Owensboro Grain Company Protix releases comparative sustainability figures for its products

NEWS FEED & ADDITIVE MAGAZINE December 2022 13 MAXIMIZE FAT ENERGY LECIMAX Improve nutrient efficiency with FRA® LeciMax Increased body weight Increased profitability Decreased FCR Read why lysolecithins in FRA® LeciMax are unique in their kind as they effectively improve fat digestion and nutrient absorption in poultry. www.adisseo.com

NEWS 14 FEED & ADDITIVE MAGAZINE December 2022 Alltech launches Egalis™ range of silage inoculants Rumin8 to test methane reducing feed additive in Brasil Alltech has announced Egalis™, a new range of high-quality silage inoculants that maximise nutrient quality retention and reduce dry matter loss. Australian climate technology company Rumin8 is set to commence a trial of its methane reducing feed additive in Brasil. The trial is due to commence in late 2022 and run for 130 days. Alltech, one of the global leaders in animal health and nutrition for more than 40 years, is proud to introduce Egalis™, a new range of high-quality silage inoculants that maximise nutrient quality retention and reduce dry matter loss so producers will have more and better silage to feed and fewer concerns about wasting feed. Egalis controls and drives forage fermentations through the use of highly efficient homolactic bacteria and elements that inhibit fungal growth. “The Egalis range of products brings together decades of expertise in fermentation, utilising bacteria strains specifically selected to maximise nutrient and dry matter protection,” said Matthew Smith, vice president at Alltech. “Together with our on-farm support teams and range of services, Alltech helps to improve fermentation to produce superior-quality silage, enabling producers to hold onto more of what they grow, from field to feed passage.” Read more>> Rumin8 announced that it will test its methane reducing feed additive in Brasil, the home of the world’s largest beef cattle herd. The company has signed a trial agreement with the Sao Paulo State University (UNESP) to undertake an extensive herd-based trial in a pasture-dominant grazing system. The trial is notable for its size with 63 performance cattle and an equal control amount for maintaining continuous grazing and stocking rates. The performance animals will be assessed for a large range of measurements (ranging from weight gain and methane reduction to nitrogen retention, microbial fermentation and change in volatile fatty acids). “The trial is led by Prof. Ricardo Andrade Reis who is world renowned for his work in the measurement of methane in grazing animals, and the technical integrity of this trial reflects that professionalism and will provide a scientifically robust data set,” said Rumin8 Managing Director David Messina. “It is an extremely detailed study which we anticipate will lay the foundation for product registration in Brasil. Brasil possesses the largest beef cattle herd in the world, so is an important potential market for Rumin8. For that reason, we have opted for a pastured-based trial to mirror the predominant beef production system in Brasil, but will extend to a feedlot-based trial at the conclusion of the initial phase.” Read more>>

NEWS FEED & ADDITIVE MAGAZINE December 2022 15 The National Industrial Hemp Council of America (NIHC) argues that hemp seed can be a sustainable and economical animal feed ingredient due to worldwide grain shortages and higher costs. The President and CEO of the Council, Patrick Atagi, in a letter urged the FDA to approve hemp seed for animal feed. The letter comes after a widely attended webinar hosted by the NIHC and the American Association of Feed Control Officials (AAFCO). The meeting brought together officials from the United States Department of Agriculture (USDA), FDA, industry stakeholders, and policymakers from across the country. The August webinar highlighted the science around animal feed and was attended by over 1,000 state regulators, veterinary professionals, and hemp industry advocates. Atagi points out the numerous clinical trials on hemp seed ingredients in animal feed by Land Grant Universities and others that have been submitted to the FDA that all show the same outcome, that there is no transference of cannabinoids into the nation’s food supply chain from animals raised on hemp seed meal. Those results have been consistent across the various species of animals, including laying hens, hogs, and dairy cattle. Atagi also pointed out that the U.S. Drug Enforcement Agency (DEA) said earlier this year that hemp seeds are not considered a controlled substance. Read more>> NIHC urges FDA to approve hemp seed for animal feed Orffa develops, customizes, sources and offers feed additives and feed additive concepts for the animal nutrition market. In short: we create, select and deliver. WE ENGINEER YOUR FEED SOLUTIONS We offer both in-house developed and branded concepts and products as well as distribution products: the ideal mix of technical products and basic feed additives. Engineering your feed solutions www.orffa.com - Follow us on Visit our NEW website WE CREATE, SELECT AND DELIVER FEED ADDITIVES

NEWS 16 FEED & ADDITIVE MAGAZINE December 2022 DSM announced that it will halt animal grade Rovimix® Vitamin A production for at least 2 months due to high costs and it will reduce Rovimix® Vitamin E-50 production. Royal DSM announced a halt to Rovimix® Vitamin A production in Sisseln, Switzerland, for at least 2 months commencing January 2, 2023. Rovimix® Vitamin E-50 production will be significantly impacted as well due to its common upstream infrastructure. At the start of the month November, DSM communicated to clients the necessity to enact price increases for its high-quality vitamins to justify continuation of production in Europe in the current challenging cost environment. With higher energy costs foreseen this winter, DSM has decided to halt Rovimix® Vitamin A production in Sisseln, Switzerland, which also impacts Rovimix® Vitamin E-50 production. Existing contractual commitments will be honored and allocation procedures have been activated across the global production network to enact this in an orderly manner. DSM is committed to sustainable production of the highest quality feed additives, including the longstanding Rovimix® product range produced in Sisseln, Switzerland, provided that aligns with our aim to generate sustainable profitable growth. DSM halts animal grade Vitamin A and reduces Vitamin E production Layn Natural Ingredients, one of the world’s largest vertically-integrated manufacturers and innovators of natural, functional botanical extract ingredients and solutions, announced the launch of TruGro® PW, an effective and proven solution validated in randomized in-vivo trials that combines selected polyphenol-rich botanical extracts to help support healthy growth and gut health in piglets post weaning. Swine producers face many challenges in the post-weaning phase as piglets transition from sow milk to solid feed. The intestinal and metabolic immaturity of piglets makes digesting solid feed more difficult, which can lead to villi atrophy, loss of barrier function, low nutrient absorption, irregular fermentation in the hindgut and pathogen microbial proliferation. Post-weaning piglets also often experience increased stress levels due to changes in nutrition and environment during this growth phase. This can lead to loss of appetite, diarrhea and stunted growth. “Traditionally, swine producers have used zinc oxide in the diet to control post-weaning diarrhea, enhance digestive function, increase secretion, improve immune response, reduce oxidative stress and control pathogens,” said Juan Javierre, Nutrition Scientist, TruGro division of Layn Natural Ingredients. “However, in June 2022, the European Union banned the use of pharmacologic doses of zinc oxide in animal feed. Layn recognized the need to provide swine producers with safe and proven alternatives.” Read more>> Layn Natural launches TruGro PW for post-weaning piglets

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ISSUE FOCUS 18 FEED & ADDITIVE MAGAZINE December 2022 NEW TRENDS IN PET FOODS AND PET FOOD INGREDIENTS

ISSUE FOCUS FEED & ADDITIVE MAGAZINE December 2022 19 Trends in petfood and road to regulatory approval within the EU Dawn Botha, Pen & Tec Consulting ADM accelerates planet-friendly pet food innovations Jorge Martínez, Pet Nutrition, ADM Improving pet food digestibility with yucca schidigera Emily Dickson, Alltech Inc Novel and green human-grade pet food formulation landscape Behnaz Shakersain, PhD, AstaReal Sweden The natural way to support a pet’s gut health Dr Maygane Ronsmans, BENEO Fiber innovations Time to shine Dr. Astrid Bosse, JRS Petfood & Aquafeed Why is it important to give your dog weight control? Loi Lee, Lachance Group

ISSUE FOCUS 20 FEED & ADDITIVE MAGAZINE December 2022 "It is obvious that pet parents want the best for their four-legged companions. But sometimes it is not so obvious to owners what they need. With the humanization of pets, comes the danger of thinking that “human-grade food” would be safe and even healthier for pets. This is not necessarily true." Cats and dogs have always been very popular companions for humans, but it is a wellknown fact that during the Covid-19 pandemic, people who were able to do so, adopted pets to keep them company. There was an increase in adoptions from shelters across the world as many people faced hard lockdown restrictions by themselves and wanted company. During recent years, owning pets has become more popular as the middle class continues to grow, and households have more disposable income available. Pets have also been proven to have a calming and supportive effect on humans, something that was valued during the emotionally trying time of the pandemic. With pets becoming increasingly more humanised by their owners, so does their diet. In this article, we´ll take a closer look at some of the trends we can expect in the future and where the European regulatory authorities stand on these. CONSUMER NEEDS The consumer, in this instance will be the pet, as well as the pet owners. It is obvious that pet parents want the best for their four-legged companions. But sometimes it is not so obvious to owners what they need. With the humanization of pets, comes the danger of thinking that “human-grade food” would be safe and even healthier for pets. This is not necessarily true. Small animal veterinary practices deal with many cases each day of owners feeding their pets a little nibble from the dinner table thinking it is harmless. The consequences can be acute, or chronic in the case of severely overweight pets. There is a vast number of pet-customers to whom pet food companies need to tailor their diets for. Owners want convenience, tailored diets, simple and healthy ingredient lists and in novel food formats. The importance of a balanced diet will always be at the forefront for pet owners. This goes without saying that pet food companies can no longer get by with having just the basic kibble on offer. Although this is the easiest choice when starting out, it is not as simple as picking up a bag at your local grocery store anymore. Pets need diets based on their size, level of activity, age, and very often breed specific. TRENDS IN PETFOOD AND ROAD TO REGULATORY APPROVAL WITHIN THE EU Dawn Botha Technical Marketing Manager Pen & Tec Consulting

ISSUE FOCUS FEED & ADDITIVE MAGAZINE December 2022 21 The industry now provides a range of pet foods adapted to the needs of all different kinds of pets. Whether they represent a daily ration (complete food) or are used in conjunction with other foods (complementary food), industrially prepared pet foods contain all the right components in carefully prescribed proportions for a pet to lead a healthy life. Pet parents are interested in convenience as well, therefore it would be in the best interest of pet food companies to make sure that their products are available via online shopping platforms, as far as possible. NOVEL FORMATS Pets are viewed as part of the family and based on that fact, consumers may seek diets containing feed additives that may benefit the pet in more ways than simply fulfilling the daily caloric requirements. There are multiple reasons for owners to supplement their pet´s normal diet. One of them might be to aid joint and mobility issues as pets become older or due to breed phenotype. Another might be to help with anxiety experienced by pets. Supplements can be added to the pet´s diet, as recommended by a veterinarian. Very often with pets, much like with ourselves, we start out with the best intentions of diligently taking the prescribed supplement, but we fall short of being 100% compliant. An innovative way to overcome this issue, is for the supplements to be incorporated into the pet food. One such example would be “calming” dog food. Formulated by veterinary nutritionists, this type of dog food already contains supplements. The owner simply needs to feed the meal as required, without the added worry of remembering to additionally include the supplement themselves. This would be very convenient if many oral medications as well as supplements could be incorporated into pet food. It is, however, not as straight forward in practice, as many of the supplements and medications are not stable during the manufacturing process. Efficacy of the supplement or medication in the finished feed is of the utmost importance. Technology is ever advancing, and the prediction is certain that there are some very exciting and innovative products in the pipeline which will be of great benefit to pets. REGULATIONS WITH RESPECT TO PET FOOD IN THE EU In the EU, pet foods are regulated the same as all animal feed which must be compliant with all relevant EU Regulations in prior to marketing including: • Regulation (EC) No 183/2005 (laying down requirements for feed hygiene)1 • Regulation (EC) No 1831/2003 (feed additive regulation)2 • Regulation (EC) No 429/2008 (detailed rules for the implementation of Regulation (EC) No 1831/2003)3

ISSUE FOCUS 22 FEED & ADDITIVE MAGAZINE December 2022 • Regulation (EC) No 767/2009 (controlling the placing on the market and use of feed)4 • Regulation (EU) No 68/2013 (catalogue of feed materials)5 It is the responsibility of the Feed Business Operator (FBO) to ensure that feed products are compliant. EU laws apply to all EU Member States and EEA (European Economic Area) countries meaning that if an ingredient has a legal status in the EU, then it can be marketed in pet foods, pet supplements and pet treats in all 27 EU Member States. The European Pet Food Industry Federation (FEDIAF) is the trade body representing the European pet food industry. 18 national trade associations and 5 companies (Affinity Petcare, Hill’s Pet Nutrition, Mars PetCare, Nestlé Purina Petcare and Wellpet) are members of FEDIAF6. Their mission is to provide safe products for the benefit of pets, and they believe that pet ownership has the ability to enhance the lives of all Europeans6. FEDIAF often co-operates closely with the EU authorities in order to work towards this goal. FEDIAF have worked to develop various guidelines centred on providing harmonised information on the regulation of pet food products in the EU. Publications include guidelines based on NRC specifications, that can be used by members of the Federation to formulate diets. These guidelines are updated regularly to ensure that manufacturers throughout Europe keep up to date with the latest in scientific and nutritional advancements. The latest version has been released in 2021. FEDIAF also developed the FEDIAF Code of Good Labelling Practice7, a user-friendly guide for consumers which provides on pet food labelling, validated by EU authorities and Member States. The Code is intended to be used by companies and authorities alongside the relevant existing EU legislation. FEDIAF also supports the sustainably production of pet food throughout Europe by the following and are committed to support recycling, ethical sourcing of raw materials and encouraging the efficient use of energy8. HOW APPROVAL IS OBTAINED Pet foods need to be approved by the European Commission (EC) to be commercially marketed. Each ingredient in the formulation needs an approval or notification, based on the type of ingredient it is. Raw feed materials generally only need a notification to be submitted to the EU authorities if not already officially listed as a feed material. A list of the registered feed materials available within the EU can be found in the above-mentioned Catalogue of Feed Materials as well as the EU Feed Materials register website. As per EU Regulation No 767/20099 complete pet food is considered to be pet food that, by reason of its composition, is sufficient for a daily ration10. Other forms of pet food can be wet food, treats and raw chews. Ingredients in these products also need to obtain approval from the regulatory bodies and final products must be compliant with the relevant feed regulations in order to be commercially marketed. As many pet foods will contain ingredients that will be considered as feed additives, these need to be approved by the EC according to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 September 2003 on additives for use in animal nutrition11. For new additives, this process requires the submission of a feed additive application to the EC followed by a scientific evaluation by the European Food Safety Authority (EFSA) before subsequent EC authorisation. It is often in the best interest of the pet food company to seek assistance with these approvals from regulatory consultancies, especially when the product is in a novel form or if the finished product contains an ingredient that for instance would be used as a treatment for an ailment or parasite. FUTURE TRENDS To stay ahead of consumer demands, the pet food industry would need to keep growing. It is estimated that around 30% of global sales are generated in Europe alone. The continent is considered one of the industry leaders in pet food and related prod-

ISSUE FOCUS FEED & ADDITIVE MAGAZINE December 2022 23 ucts12. The pet food industry remains an exciting space within the animal feed industry and the future looks bright for pets in terms of new and innovative products on the horizon. References 1. https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=CELEX%3A02005R0183-20220128 2. https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=CELEX%3A02003R1831-20210327 3. https://eur-lex.europa.eu/legal-content/GA/TXT/?uri=CELEX:32008R0429 4. https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=CELEX%3A02009R0767-20181226 5. https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=CELEX%3A02013R0068-20220724 6. https://europeanpetfood.org/about/structure/ 7. https://europeanpetfood.org/self-regulation/labelling/ 8. https://europeanpetfood.org/sustainability/sustainability-2-2/ 9. https://eur-lex.europa.eu/LexUriServ/LexUriServ. do?uri=OJ:L:2009:229:0001:0028:EN:PDF 10. https://ec.europa.eu/environment/eussd/smgp/pdf/ PEFCR_PetFood_FinalPEFCRs_2018-05-09.pdf 11. https://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:02003R1831-20210327&from=EN 12. https://europeanpetfood.org/pet-food-facts/pet-foodtrends/ About Dawn Botha Dawn Botha was born and raised in South Africa and completed her Master of Science in dairy nutrition at the University of Stellenbosch near Cape Town. Later in her career, she took an interest in marketing management and decided to add this to her skill set. She has extensive experience within the animal nutrition and animal health fields, in particular production animals, and has worked for multinational animal health companies in South Africa. She joined the team from Pen & Tec Consulting as their Technical Marketing Manager and is now based in Barcelona, Spain. Pen & Tec Consulting have recently become part of the Argenta Global group. [email protected] www.pentec-consulting.eu Training offer at: www.pentec-academy.com REGULATORY APPROVAL FOR PET FOOD & INGREDIENTS IN THE EU Seek out assistance during the application phase to avoid potential delays

ISSUE FOCUS 24 FEED & ADDITIVE MAGAZINE December 2022 Modern pet parents are looking for ways to feed their four-legged family members in much the same way that they feed themselves. According to ADM’s 2023 global trends research, this includes a preference for pet food that aligns with the popular flexitarian lifestyle, in which many sources of protein, both traditional and alternative, are used to comprise a more sustainable and earth-friendly diet. Additionally, exactly what ingredients are used in their pets’ food, where it comes from, who made it and how it was made are of growing importance to both North American and global consumers. To meet these needs, ADM Ventures, the corporate venture capital division of ADM, has made two new important partnerships to support the development of expanded protein sources and earth-first production practices. Bond Pet Foods is a pioneer at the forefront of alternative protein, employing precision fermentation to make nature-identical chicken, beef, fish and other meat proteins for pet food applications. Innovafeed, a leader in the growing space of insect proteins, has taken advantage of the approval by the Association of American Feed Control Officials (AAFCO) for black soldier fly larvae (BSFL) to be used in adult dog food and treats in the U.S. ADM’s investment in these two forward-thinking companies represents its commitment to producing pet food the right way, to benefit pets, people and the planet. PRECISION FERMENTATION AS A PET PARENT PREFERRED SOLUTION The 163 million dogs and cats that comprise America’s pet population eat an enormous amount of protein. As the world’s population continues to increase, responsible protein production is of critical importance, particularly for animal protein which has a significant impact on the planet’s land, water and energy resources. Bond Pet Foods is solving this problem by applying precision fermentation to produce nutritionally complete nature-identical meat proteins for dogs and cats that are far less resource intensive than their animal protein counterparts. “We’re excited to be a part of this next chapter of Bond Pet Foods’ growth,” said Jorge Martínez, President, Pet Nutrition for ADM. “More and more, consumers demand their pets eat the same sustainable, responsibly produced food that they themselves eat, and proteins produced by precision fermentation are key to being part of the future of the industry. We’ve expanded our capabilities and are proud to be part of the Bond Pet Foods journey.” Precision fermentation, Bond’s production proJorge Martínez President Pet Nutrition, ADM ADM ACCELERATES PLANET-FRIENDLY PET FOOD INNOVATIONS ADM Ventures invests in two startups on the leading edge of developing alternative proteins for pet food, including animal-free proteins created by precision fermentation and alternative protein made from insects.

ISSUE FOCUS FEED & ADDITIVE MAGAZINE December 2022 25 cess, is similar to industry-standard practices for making widely used ingredients like lactic acid, vitamin B12 and enzymes for cheese production. These processes are safe and established and require no animal farming. For reasons like these, animal-free protein is perceived as sustainable and humane by modern pet parents. ADM’s funding will contribute to significantly expanded capacity and capabilities in Bond’s Boulder, Colorado operations with ground having recently been broken on a new 15,000 sq. ft. facility. “With their support,” added Rich Kelleman, CEO and Co-Founder of Bond Pet Foods, “we’ll be able to take our work to the next level, developing and scaling a variety of meat proteins for our valued partners in the pet food space.” INSECT PROTEIN FOR EARTH-FRIENDLY PET FOOD The ADM/Innovafeed partnership includes the construction of a new Innovafeed production site next to ADM’s grain processing plant and headquarters in Decatur, Illinois. The location of this strategically placed facility will allow Innovafeed to employ its circular industrial symbiosis model. Not only will ADM provide feedstock for BSFL but also waste heat and water, which Innovafeed’s plant will upcycle to reduce reliance on fossil fuels. Additionally, the proximity of the facilities inherent in this model reduces the energy costs of transportation, saving approximately 120 gigawatt hours (GWh) per year, or the annual energy consumption of more than 10,000 households. This collaboration also aims to commercialize the use of dried whole black soldier fly larvae as an alternative protein for the North American pet food industry. BSFL oil, already tentatively approved for adult dogs in the U.S. and approved by the European Food Safety Authority (EFSA) for use in dog and cat food, is also being developed as a source of fat for pet nutrition products. Furthermore, a pilot research facility is being constructed in Decatur to allow for joint research and development on pet food formulation, such as palatability studies and protein performance. “Our pet nutrition experts will collaborate with the Innovafeed team and other research scientists to unlock the benefits of black soldier fly lar-

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