ISSUE FOCUS 46 FEED & ADDITIVE MAGAZINE November 2022 week after weaning, then groups positive control and FA-esters were inoculated with SS9 (isolate 2524) via oral and nasal routes with 3 mL at the dose of 1.0 x 109 CFU/mL per route. Piglets from negative control receiving phosphate buffer saline instead. Results showed that SS9 concentration decreased in negative control and +FA esters, from 4.0 to 2.2 and 4.9 to 3.2 Log10 CFU.mL-1, respectively. In +C, SS9 concentration remained constant (5.4 Log10 CFU.mL-1) (Figure 3). This trial highlighted the beneficial effects of fatty esters supplementation to reduce S. suis colonization in piglet tonsils. BENEFITS IN POULTRY: FATTY ACID ESTERS CONTRIBUTE TO GUT HEALTH BY THEIR EFFECTS ON MICROBIOTA AND INTESTINAL CELLS Through an in vivo trial performed on a necrotic enteritis (NE) model on broilers, comparing 4 groups on 110 animals per group: Control (infected untreated), Positive Control (control with antibiotic), 2 groups with low and high dosage fatty acid esters- supplementation, it has been observed a positive effect of supplementation on the reduction of Clostridium perfringens. Microbiota relative abundance of Clostridium was reduced by an average of 30% and 50% in fatty acid esters groups, respectively, compared to control. In treated group, antibiotic was highly efficient toward Clostridium spp. (less than 0.05% of abundance). However, the beneficial Lactobacillus bacteria were also significantly reduced (71% relative abundance vs 91.3% in control), associated with an increase of the genus Corynebacterium, Staphylococcus, Shigella and E. coli, that represented respectively above 7%, 10%, 4.5% and 2.5% of the microbiota. Fatty acid esters supplementation limited the predominance of these opportunistic bacteria (Figure 4). In concert with the antimicrobial effect and their ability to balance the microbiota with the maintaining of the beneficial microflora, inclusion of fatty acid esters has shown direct effects on the histomorphology of the gut by increasing the villus length (Figure 5). Herein, supplementation of fatty acids esters may facilitate the nutrient absorption, maximizing nutrient utilization and that in turn growth performance in broiler chicken (-0.02 to 0.04 pts of ICC). CONCLUSION With growing evidence, fatty acids esters solutions appeared as useful in preventing and mitigating microFigure 3. Streptococcus suis (SS9) concentration in swine tonsils (Log10 CFU.mL-1) (*) Treatment X day interaction (P<0.001)
RkJQdWJsaXNoZXIy MTUxNjkxNQ==