ISSUE FOCUS 40 FEED & ADDITIVE MAGAZINE August 2022 test was performed using a pH of 5.0 for adsorption and 6.5 for desorption. The results of this in vitro test have been corroborated on several occasions (Graph 1). IN VIVO ENDOTOXIN BINDING These in vitro results demonstrate the potential capacity of a binder to capture toxins. Still, the fact remains that a product needs to prove effective in the animal. A recent ex vivo study by the University of Ghent effectively proved the capacity of a toxin binder to capture endotoxins in the intestine of piglets (Graph 2). In the study, the authors compared the effect on the production of cytokines by injection of endotoxins with or without the addition of the toxin binder. The model was intestinal loops of live piglets (Figure 3). Endotoxins have a wide variety of effects on livestock, including depressed feed intake, fatty liver, and loose feces to name a few. Different means to control endotoxins have been tested but most of them are too expensive to be considered in animal production. A cost-effective method is the use of Figure 3: In two 5-week-old piglets, 3 intestinal loops per treatment of 8 cm were produced at the level of the jejenum. Different conditions were then injected directly in the intestinal loop and a biopt of each look was taken to determine endotoxin binding potential Method of the in vivo study In the trial we had 4 different treatments: T1: 0.15% Vitafix Spectrum + 5,000 EU LPS T2: 0.15% Vitafix Spectrum + 100,000 EU LPS T3: 0.3% Vitafix Spectrum + 5,000 EU LPS T4: 0.3% Vitafix Spectrum + 100,000 EU LPS LPS binding efficiency LPS adsorption % - LPS desorption % Measured after 10 minutes, 1 hour and 3 hours Graph 1: The results of the in vitro test show the high binding capacity of Agrimprove’s toxin binder at high LPS concentrations in a short period of time Screening the endotoxin binding efficiency of Agrimprove’s toxin binder at high endotoxin concentrations
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