ISSUE FOCUS FEED & ADDITIVE MAGAZINE March 2022 49 SOLUTION: PRESERVE GRAM+ BACTERIA LEVELS WHILE DECREASING FREE LPS We have therefore seen how important it is to decrease the acetic:propionic ratio in the rumen to obtain a more significant share of available energy. However, the level of endotoxins in the rumen must remain low in order to avoid those problems of endotoxemia linked to very specific pathologies typical of "super productive cows". These pathologies (always linked to inflammatory manifestations) can be prevented by decreasing the level of free LPS in the rumen with a product that can irreversibly bind the LPS and thus make them inactive. In a trial with porcine intestinal cells (IPEC-J2) challenged by E. coli LPS, a decrease in the intensity of inflammation was observed when Mastersorb Gold was added. This decrease could be shown through a lower amount of phosphorylated NF-kB in an immunofluorescence trial, as well as through the reduced production of Interleukin (IL)-8 in the cells measured by ELISA. The fact that pig intestine tissue was used does not affect the adsorption concept. In this case, these intestinal cells are only a vehicle to demonstrate that in an aqueous solution containing 50 ŋg of LPS / ml and in the same solution with the addition of Mastersorb Gold, the level of LPS actually active is decreased, as a part of the LPS was tied up by Mastersorb. The solution with a lower level of LPS gave minor "inflammatory" reactions to intestinal cells, and this can be statistically reported in dairy cows. CONCLUSIONS To demonstrate how the decrease in the level of LPS in the rumen is directly correlated with inflammatory states in general, a trial with a total of 60 dairy cows shows that the inclusion of 25g of MasFigure 3. Effect of Activo Premium on ruminal constituents Figure 4. Effect of Activo Premium on animal performance
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