Feed & Additive Magazine Issue 14 March 2022

March 2022 Year: 2 - Issue: 14 Valérie Kromm, Animine Copper in ruminants: From essential to toxic Katrien D’hooghe, BFA Belgian feed industry and trends Stefano Vandoni, Balchem Different encapsulation techniques lead to various nutrient bioavailability www.feedandadditive.com The Importance and Optimization of Particle Size in Feed Production Ways to Improve Performance in Dairy Animals

The world is like a volcano that has been boiling inside for many years and waiting for the 2000s to erupt… It has been continuing to unleash all its lava on us for the last few years. Outbreaks, pandemics, climate change, problems of access to food, economic depressions, wars… The peoples of the world have to face another problem, even before they are able to overcome one of them yet. Of course, being both the protagonist and the victim of an important part of these problems is a strange situation. First, to wreak havoc on the entire natural balance of the planet with unsustainable methods, and then to face the destructive effects of this disturbed balance... I guess this is what “reaping what you sow” means... Although many of us today have realized that we cannot continue to use existing resources carelessly, and to produce and consume unconsciously; it is not easy to change the way we had started to build. Moreover, it is a big problem that not everyone is in the same effort to change this path. We are on a planet where there are still those who hold the real agenda of the world behind their own individual agenda. On the big screens, on the one hand, we talk about the globalization of the world eloquently, and that the sharpness at the borders reduced, and on the other hand, the importance of freedom and independence. However, we continue to sharpen the boundaries with the power struggles of the hidden and visible actors in the background. I think the Russia-Ukraine war is the most obvious example of this. It is possible to understand economic and political wars to some extent, but armed conflicts, which have no other result than harming completely innocent civilian population, should have no place in today's world. In my opinion, armed warfare is the greatest anomaly of our age. I hope that this outdated method will soon leave its place to a peaceful dialogue that is more appropriate for our age. In Ukraine, in Syria, in Afghanistan, wherever it is around the world, there is never a winner in an armed warfare; there is always a loser... Not only two actively belligerent countries pay the price for this, but the whole world, directly or indirectly… I hope the world can move towards the days when we can write more beautiful and hopeful articles. See you in the next issue. Publisher Muhammed Akatay [email protected] Responsible Manager Özlem Buyruk Akatay [email protected] Editor-in-Chief Derya Yıldız [email protected] Editors James Watson Bahadır Büyükkılıç [email protected] Design Alper Kandemir [email protected] Web Design Caner Cem Pulat [email protected] Proofreading Lisa Atakul [email protected] Circulation and Data Responsible Amine Şengün [email protected] Ataturk St. Metropol Istanbul Ste. Block C1, No:376 Atasehir, Istanbul/TURKEY Phone: +90 850 220 5064 E-Mail: [email protected] Web: www.feedandadditive.com Branch: Ste B69, East Rutherford, New Jersey 07073, USA Publication Type: Periodic (Monthly) EDITOR The era of conflicts Derya YILDIZ All copyrights belong to Three Plus Media.© May not be used without permission. Responsibility of the ads belongs to the advertisers. feedandadd i t i ve

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66 DIFFERENT ENCAPSULATION TECHNIQUES LEAD TO VARYING LEVELS OF NUTRIENT BIOAVAILABILITY Stefano Vandoni, PhD, Technical Service Manager, Balchem ANH, EMEA ARTICLES C O N T E N T S 16 ISSUE FOCUS 18 IMPROVING FIBRE DIGESTIBILITY TO UNLOCK 3 Es IN DAIRY PRODUCTION Jac Bergman, DVM, Global Programme Manager Ruminants, Selko Feed Additives 24 MILK PROFIT: IT CAN ALWAYS BE BETTER Valentin Nenov, Global Ruminant Manager, Phileo by Lesaffre 28 ENHANCING EARLY LACTATION IN COWS THROUGH PLANT EXTRACTS Sebastien Constantin, Business Development Manager Phytogenic Bioactives, Pancosma 40 PHYTOGENICS: NATURAL ADDITIVES TO IMPROVE PERFORMANCE IN DAIRY Delphine Lacombe, Global Technical Manager Ruminants, Delacon Biotechnik GmbH 32 COPPER IN RUMINANTS: FROM ESSENTIAL TO TOXIC Valérie Kromm, Product Manager, Animine 46 HOW DO ENDOTOXINS AND RUMEN HEALTH INFLUENCE COW PERFORMANCE? Claudio Campanelli, Technical Manager – Ruminants, EW Nutrition 36 A SUCCESSFUL LACTATION IS A MATTER OF PREPARATION! Miriam Deppe, Product Manager Ruminants - Dietary Feed Supplements, Biochem 51 PROTECTED EXTRACTS FROM PLANT AND SEAWEED TO SECURE... Dr. Stephanie Ladirat, R&D Director, NUQO Feed Additives WAYS TO IMPROVE PERFORMANCE IN DAIRY ANIMALS

C O N T E N T S BELGIAN FEED INDUSTRY AND TRENDS 54 INTERVIEW Katrien D’hooghe, Managing Director, Belgian Feed Association (BFA) ADM releases 2022 alternative protein outlook 12 Fishmeal production up in 2021, fish oil production drops 8 International Egg Commission takes action to double egg consumption globally 74 ARBIOM receives €12 million to build its first commercial plant 76 Protix first insect genetic selection program yields incredible results 78 Annual DSM Mycotoxin Survey points to high risk level 81 BioMar increases amount of microalgae use in feed production 83 SPECIAL STORY 58 IMPORTANCE & OPTIMIZATION OF PARTICLE SIZE IN ANIMAL FEED PRODUCTION Arthur vom Hofe, Segment Manager Feed & Oilseeds, CPM Europe 62 THE IMPORTANCE OF FEED PARTICLE SIZE (GRANULOMETRY) IN LAYING HENS Estella Leentfaar, Nutritionist, Hendrix Genetics Layers Particle Size in Feed Production REPORTS GLOBAL FEED AMINO ACIDS MARKET 69 NEWS

NEWS 6 FEED & ADDITIVE MAGAZINE March 2022 Evonik announced that it will build a methyl mercaptan plant at its site in Mobile, Alabama in the U.S. Methyl mercaptan is an intermediate in the production of MetAMINO® (DL-methionine) and is currently sourced from third parties. This backward integration step strengthens Evonik's site in Mobile as a global, best-in-class methionine hub for reliable and cost-optimized supply to North and South American markets. The plant is scheduled to come on stream in the second half of 2024. The investment budget of the project is approximately EUR 150 million over three years. "The new methyl mercaptan plant in Mobile is another important step in the consistent implementation of our global methionine asset strategy," says Johann-Caspar Gammelin, head of EvonEvonik strengthens world-scale methionine production hub in U.S. Evonik will build a methyl mercaptan plant in Alabama, U.S. The investment budget of the project is around 150 million Euros over three years. Phileo by Lesaffre announced that the company will invest in a new factory in Valladolid, Spain for the production of Selsaf®, Phileo’s nutritional solution enriched in organic selenium. Committed to a sustainable agrifood industry and to answer the growing demand, Phileo by Lesaffre announced the Lesaffre investment in a new factory in Valladolid, Spain for the production of Selsaf®, Phileo’s nutritional solution enriched in organic selenium. Last fall, the construction of a new factory began and the first phase is expected to be completed next summer. For the year 2023, a second phase of investments is planned that will allow the increase of the production capacity in the face of the growing demand for these solutions. “The new facilities dedicated to the production of Selsaf® aim at satisfying a growing demand for these products in Europe, Africa and the Middle East and allow for local sourcing,” says Claire Jollet, Western Europe Director of Phileo by Lesaffre. “We hope to start delivering from the new plant by the end of the year,” she adds. Selsaf® helps the body against oxidative stress (which is detrimental to animal performance), both in the short term (thanks to the selenocysteine) and in the long-term (due to the selenomethionine) and boosts natural defenses of animals. This dual protection leads to dual benefits to farmers and consumers. For farmers, Selsaf® supports animal health (better embryo viability and survival rate, more selenium transfer to offspring, less morbidity) and increases animal performance (higher feed efficiency, growth performance, laying intensity…). In reducing the oxidation, and therefore the rancidity of animal-derived end-products, Selsaf® also brings a lot of benefits to consumers, with more selenium in meat, milk and eggs, and more food pleasure with increased meat quality (less drip loss, increased juiciness and tenderness). Phileo by Lesaffre to invest in new factory in Spain

NEWS FEED & ADDITIVE MAGAZINE March 2022 7 ik's Nutrition & Care division. "We are securing our cash flow from the methionine business to finance our growth with system solutions in Nutrition & Care." The core of the methionine asset strategy are the three world-class production hubs: Mobile, Antwerp (Belgium) and Singapore. Together these hubs serve the expanding global market for DL-methionine. As part of the asset strategy, the smallest methionine plant at the German site in Wesseling was discontinued in 2021. At the same time the European MetAMINO® Verbund was strengthened by investment in intermediates at this site. The global methionine production hubs in Antwerp and Singapore are already fully backward integrated. This contributes – just like the present investment in Mobile – to improved supply security and a market-leading cost position. It also facilitates future and highly efficient debottlenecking over the next years. The investment is also a building block of the division's sustainability strategy: it will reduce the carbon footprint of DL-methionine from Mobile by about seven percent. More than 25,000 metric tons of CO2 equivalents can be saved each year. Methionine is used in livestock farming to feed animals healthily, efficiently, and sustainably. Nutreco and Holmøy Group to make strategic investments in Andfjord Salmon Nutreco, a global leader in animal nutrition and aquafeed, and Norwegian seafood group Holmøy, have agreed to invest in Andfjord Salmon, as part of an approximately $4.3 million private placement. Nutreco, a global leader in animal nutrition and aquafeed, and Norwegian seafood group Holmøy, have agreed to invest in Andfjord Salmon, as part of a NOK 38 million (approximately $4.3 million) private placement. In addition, Andfjord Salmon has entered into an operational feed supply agreement with Nutreco’s aquaculture business line, Skretting. Nutreco, with over 12,000 employees in 37 different countries, is a global leader in animal nutrition and aquafeed, with advanced nutritional solutions at the origin of food for millions of consumers worldwide. In Norway, Nutreco operates through its aquaculture business line, Skretting. Holmøy is a seafood group based in Vesterålen, North Norway. It is one of Norway’s largest players within whitefish and salmon. Holmøy group will invest in Andfjord Salmon through its fish farming company Eidsfjord Sjøfarm AS, which already owns 66,400 shares in Andfjord Salmon. Nutreco will invest NOK 20 million in Andfjord Salmon, while Eidsfjord Sjøfarm will invest NOK 8 million. In conjunction with investment, Andfjord Salmon has entered into a long-term, running strategic feed supply agreement with Skretting. The feed supply volume will be adapted on an ongoing basis to Andfjord Salmon’s production volumes of Atlantic salmon. Under the agreement, Skretting commits to supply a feed that is specifically tailored to Andfjord Salmon’s flow-through technology to ensure optimal water quality, fish welfare and growth conditions in the land-based pool.

NEWS 8 FEED & ADDITIVE MAGAZINE March 2022 Royal DSM announced that Bovaer®, methane-reducing feed additive for dairy cows, has been approved for marketing in the EU. This marks a significant milestone for the company. According to the market reports prepared by IFFO, fishmeal production increased by 3.6%, while fish oil production dropped by 6% in the JanuaryDecember 2021 period. Fishmeal production up in 2021, fish oil production drops DSM receives landmark EU market approval for Bovaer® The Marine Ingredients Organisation (IFFO), the international trade organisation that represents the marine ingredients industry, released its market intelligence report. According to the report, in the January-December 2021 period, fishmeal production Royal DSM announced that European Union (EU) member states approved the marketing of the methane-reducing feed additive for dairy cows, Bovaer®, in the EU. After inclusion in the EU registry, expected in the coming weeks, it is the first time a feed additive authorised in the EU for environmental benefits can be marketed. This marks a significant milestone for DSM, paving the way for Bovaer® to revolutionize the dairy market. Methane has a global warming potential 28 times greater than carbon dioxide, which is why reducing methane emissions was identified at COP26 as one of the most significant short-term means of tackling climate change. More than 100 governments pledged to cut methane emissions by 30% by 2030, as part of the Global Methane Pledge, launched by the EU and the US. Bovaer® will contribute to the greening of the EU's agriculture, and to the objectives of the Farm to Fork Strategy. As stated in the European Commission's confirmation of approval, the innovative feed additive is safe for use without impacting the quality of dairy products and is the first of its kind to be available within the EU which can reduce methane emissions. The dairy industry has an important role to play as enteric methane from dairy cows is responsible for up to 60% of the global greenhouse gas emissions from milk production. DSM's ruminant feed additive Bovaer® consistently reduces these methane emissions by around 30%. Bovaer® is the result of a decade of scientific research, including more than 50 peer-reviewed studies published in independent scientific journals and 48 on-farm trials in 14 countries across 4 continents. DSM has entered into partnerships with several major dairy companies to prepare for the implementation of Bovaer® at large scale. DSM has also already begun engineering works for a large-scale Bovaer® plant at DSM's manufacturing site in Dalry, Scotland.

NEWS FEED & ADDITIVE MAGAZINE March 2022 9 Supplant D Health and performance go hand in hand • Improved health status • Stable rumen environment • Smoother transition • Reduced somatic cell count agrimprove.com Aromabiotic® Cattle increased by 3.6%, while fish oil production dropped by 6%. The total fishmeal production of the countries analysed in the IFFO reports is estimated at 2.443 million metric tonnes (mt) in 2021, compared to 2.359 million mt reported in 2020. Peru, Chile and India increased their cumulative fishmeal output in 2021, while the Northern European countries, the USA and African countries reported a collective decrease of more than 160,000 mt, year on year. These countries make up approximately 50% of the world production, and can be taken as a proxy for global trends. In terms of fish oil, on the contrary, the overall output of the countries analysed in the IFFO report has dropped by almost 6%. The total output in 2021 is currently estimated at 546,000 mt, compared to 581,000 mt in 2020. Chile, Iceland and India were the countries that have improved their production in 2021, while all the other areas, including Peru, the key producer, reported a contraction. From January to April 2022, little production of marine ingredients is expected, and most of it is expected to be based on by-products. The record level of fishmeal imports reported in 2021 might be reached again in 2022. The demand of marine ingredients is expected to be still dominated by aquaculture, in a context where Chinese authorities have committed to managing the sector according to strict environmentally protective criteria. China’s aquaculture currently remains off-season. The average trading price of aquatic products rose in December 2021 and January 2022 year on year, supported by healthy trading volumes. The farmgate prices of farmed species rose before the Spring Festival holidays as expected. The pig sector continues to suffer from over-capacity, leaving the price of live pigs still under pressure. The Spring Festival holidays, which traditionally imply a higher consumption of pig meat, may only provide a short-lived respite.

NEWS 10 FEED & ADDITIVE MAGAZINE March 2022 Singapore-based alternative protein company INSEACT opened the doors to its facility in Singapore, in a ceremony attended by government representatives, investors and industry partners. After receiving the keys to the completely empty site in September of last year, INSEACT completed construction and equipment installation and set up production and R&D activities in only 4 months, INSEACT launches Singapore’s largest insect protein facility Alternative protein company INSEACT has opened Singapore's most advanced and largest insect protein facility. Kerry acquires biotechnology companies c-LEcta and Enmex Kerry, one of the world's leading flavor and nutrition companies, has announced that it has made two significant biotechnology acquisitions that will expand its expertise, technology portfolio and manufacturing capabilities. The company has reached agreement to acquire the leading biotechnology innovation company, c-LEcta while also confirming that it has acquired the Mexican enzyme manufacturer, Enmex. c-LEcta is a leading biotechnology innovation company specialising in precision fermentation, optimised bio-processing and bio-transformation for the creation of high-value targeted enzymes and ingredients. Based in Leipzig, Germany and employing over 100 people, c-LEcta has established itself as a leading innovator in disruptive new sciences for the pharmaceutical market, with a strong pipeline of functional bioactives across food, beverage and other consumer markets. Enmex is a well-established enzyme manufacturer based in Mexico, supplying multiple bio-process solutions for food, beverage and animal nutrition markets. With a long history of partnering with global customers, Enmex has a complementary enzyme portfolio and a strong manufacturing infrastructure, which will extend Kerry’s fermentation and enzyme manufacturing capabilities into Latin America. Kerry announced two significant strategic biotechnology acquisitions, leading German biotechnology innovation company c-LEcta and Mexican enzyme manufacturer Enmex.

NEWS FEED & ADDITIVE MAGAZINE March 2022 11 while remaining 10% under budget. In just the first few weeks of ramping up in December 2021, the colony produced 60 million insects. INSEACT specialises in producing sustainable insect protein for aquaculture, starting with shrimp farming. It uses waste from palm oil operations as a raw material to feed the insects. Its cutting-edge solution enables a fully circular economy by redirecting waste streams from palm oil production away from carbon-emitting landfills to be bio-converted by the insects. The new facility is operated by INSEACT’s growing team of 8 employees, half of whom have extensive prior insect protein experience. The facility adapts engineering technologies used across other manufacturing and farming industries to lay the foundation for large scale expansion of its operations. Michael Badeski, COO, said: “The past 12 months have seen INSEACT go from zero to one. Last January, the company had no employees, only partially secured seed funding. Today, we are producing insect protein from the most advanced and fastest-built insect pilot facility in Singapore.” Tim van Vliet, CEO of INSEACT, added: “INSEACT differentiates itself using palm oil waste: a more sustainable, affordable and scalable feedstock. In addition, our modular technology enables fast and low-cost scaling.” The insect start-up Enorm Biofactory has closed a funding round of 50 million Euros. With new partners, a strong team, and permits in place, Enorm Biofactory will shortly scale up the insect factory in the middle of Jutland from pilot plant to a yearly production of application 11,000 tons of insect meal. As soon as February, Enorm Biofactory will start building the first new square meters for the future factory in Hedensted Municipality, Denmark. When the new site is in full production in the second half of 2023, 100 tons of larval biomass per day will be produced, equivalent to application 11,000 tons of insect protein meal per year. Closing the funding round is a major milestone for the company to be able to realize the project. Among new shareholders is, one of Europe’s largest agribusiness companies, DLG. The financing round also includes loans from The Danish Green Investment Fund and Nykredit Bank. “With our current suppliers and cooperation partners, and DLG as a shareholder, we have a strong foundation to develop Enorm to commercial scale” says Carsten Lind Pedersen, CEO at Enorm Biofactory. Enorm Biofactory was established in 2017 and is run by Carsten L. Pedersen (CEO, 59), his daughter, Jane L. Sam (COO, 33), Arne Blok Lauridsen (CTO, 32) and additional 13 employees. For the last 3 years, Enorm Biofactory has established a pilot plant and is today producing 3 tons of insect meal per week. The planned scale up is therefore app. 70 times the current production. Enorm Biofactory to build largest insect factory in Scandinavia Danish insect producer Enorm Biofactory announced that it closed its funding round and ready to build the largest insect factory in Scandinavia.

NEWS 12 FEED & ADDITIVE MAGAZINE March 2022 ADM released its alternative protein outlook for 2022, revealing what’s next for protein alternatives, which are expected to climb to a staggering $125 billion by 2030. ADM, a global leader in nutrition that powers many of the world’s top food, beverage, health and wellness brands, released its alternative protein outlook. The protein outlook is a deeper dive into one of the seven top consumer trends identified by the company for 2022. The findings, based on research from the company’s proprietary Outside VoiceSM consumer insights platform, reveal what’s next for protein alternatives, which are expected to climb to a staggering $125 billion by 2030. “In the last 12 months, the number of plant-based meat, cheese and dairy products available to consumers has more than doubled,” says Leticia Goncalves, president of Global Foods for ADM, who oversees the company’s focus on high-growth business. “Innovation driven by future-forward brands like Air Protein, Future Meat Technologies, Nature’s Fynd, in partnership with larger industry players such as ADM, are transforming the way we will feed a growing global population sustainably. Products aimed at meeting the heightened demand for health-forward solutions that can deliver on evolving consumer taste and texture expectations will continue to come to market over the next decade,” she adds. ADM releases 2022 alternative protein outlook Aiming to reshape the future of protein production, Nutreco announced that it launched the 2022 Nutreco Feed & Food Tech Challenge (NFFTC) to find startups and other pioneers with innovative and disruptive ideas. Nutreco is looking for startups and other pioneers with innovative and disruptive ideas to produce more food with fewer resources. Candidates can now apply to 2022 Nutreco Feed & Food Tech Challenge (NFFTC). Start-ups across the globe are invited to submit their applications. The challenge focuses on breakthrough innovations within livestock farming, aquaculture and alternative food proteins. The winning start-up receives a scientific validation trial at one of Nutreco’s research centers. Nutreco experts will select 15 teams to receive coaching by industry experts and to pitch virtually to the jury in June. The jury will consist of Nutreco and industry experts. The top five teams will then be invited to an in-person event in the Netherlands later in the year. Applications need to be sent by April 25 at 12:00 CET. Requirements for the pitch deck can be found on the Nutreco website. “Feeding the Future will require significant technological innovation. A single idea could make a huge impact on the future of protein and our planet,” says Joost Matthijssen, Nutreco’s Director of Venturing and NFFTC head juror. Nutreco looks for startups with innovative ideas

NEWS FEED & ADDITIVE MAGAZINE March 2022 13 Alltech announced that the ONE Conference (ONE) will be held May 22-24 in Kentucky, USA both in person and virtually on a first-class platform. The Alltech ONE Conference (ONE) will return to Lexington, Kentucky for a collaborative exploration of the challenges and opportunities in the agri-food industry and beyond. Alltech, a global leader in animal health and nutrition, announced that their 38th annual flagship event will be held May 22-24 both in person and virtually on a firstclass platform, with live-streaming and on-demand presentations available to ensure accessibility to everyone, everywhere. Reflecting the most relevant topics impacting the agri-food industry, the key themes of the discussions held at ONE will include science, sustainability and storytelling. ONE attendees will derive inspiration from keynote speakers who have unleashed the power of innovation and courageous leadership for positive impact. While the world-class keynote speakers at ONE will offer universally valuable insights, the subject- and species-specific tracks will explore emerging opportunities in aquaculture, beef, crop science, dairy, pig, poultry, equine, health and wellness, business, and brewing and distilling. Confirmed speakers are currently listed on the ONE website, with more to be added. Virtual attendees of ONE will have access to live-streamed keynotes and pre-recorded track presentations that can be viewed on-demand. For in-person attendees, the ONE experience will extend into beautiful downtown Lexington, Kentucky, with special events, dinners and tours. Alltech ONE Conference (ONE) returns to Kentucky, May 22-24

NEWS 14 FEED & ADDITIVE MAGAZINE March 2022 MarinBio to take part in €5-million EU project Turkish company MarinBiotechnology (MarinBio), known for its microalgae and probiotic production, announced that it will take part in a European Union project with a budget of 5 million 95 thousand euros, together with Mugla Sitki Kocman University. Within the scope of the “Climate smart, ecosystem-enhancing and knowledge-based rural expertise training centers (RURALITIES)” project, regional development-oriented trainings will be provided in the fields of agriculture, livestock and aquaculture. Studies will continue for 60 months for the project with the code HORIZON-CL6-2021-COMMUNITIES-01, which has 54 partners. Istituto Di Istruzione Superiore A. Cecchi from Italy will be the coordinator of the project. MarinBio, which is the leader in Turkey with a monthly production of 10 tons of microalgae, is also preparing to establish a new facility. The company, which plans to produce algae oil in its new facility, aims to operate not only in agriculture and animal husbandry, but also in different fields such as human nutrition and cosmetics. Görkem Koyun, the founder of Marin Biotechnology, says, "I think that the AlgomeTM product, which has a high lubrication feature, will attract attention by individuals on a Vegan-Vegetarian diet due to its rich Omega-3 & DHA profile in its oil content". Expressing that algae, which can be used in about 15 different industries, will have a different importance in the future thanks to its sustainable production model and high quality nutritional content, Koyun emphasizes that microalgae should be supported to the end and its production increased. “We produce with completely biotechnological methods and there is no waste material as a result of production. We carry out an environmentally friendly and efficient production,” Koyun adds. MarinBio, a Turkish company that makes significant contributions to sustainable agriculture and animal husbandry with biotechnological methods, announced that it will participate in a European Union project with a budget of 5 million euros. Swedish startup Volta Greentech announced the results of its pilot on a commercial beef farm. The practice showed that methane emissions were reduced by on average 81 percent in animals fed the seaweed-based feed supplement. Volta Greentech showed in a pilot at a commercial beef farm that methane emissions burped out by the animals were reduced by on average 81% when fed the company’s seaweed-based feed supplement. In the pilot at the farm “Tre Bönder’’ outside Eskilstuna in Sweden, 17 animals were fed the seaweed-based feed supplement as part of their diet to reduce methane production. The seaweed comes from Volta Greentech’s land-based pilot factory in Lysekil. The feed suppleSeaweed cuts methane emissions by over 80%

NEWS FEED & ADDITIVE MAGAZINE March 2022 15 The distribution of Bioiberica's Palbio™ range through Mill Tech Co., Inc. in South Korea has been approved after fulfilling stringent regulatory requirements. Spain-based Bioiberica, a global Life Science company committed to improving the health and well-being of people, animals and plants, announced a new strategic agreement with Mill Tech Co., Inc. for the exclusive distribution of its hydrolysed protein range for animal nutrition, Palbio™, in South Korea. The agreement comes after the successful fulfilment of rigorous export requirements to the country, enabled by Bioiberica’s robust production process, which ensures the biosecurity of its entire animal nutrition portfolio. This move marks a milestone in the companies’ long-standing strategic partnership, solidifying their collective offering in South Korea. The companies have been working together for several years to distribute Bioiberica’s Nucleoforce™ range, used to support the immunological and intestinal health of farm animals and aquaculture species. Palbio™ 50 RD is now authorised for distribution in more than 40 countries, including the recent addition of South Korea. It is a safe source of high-quality hydrolysed proteins in the form of L-α-amino acids and bioactive peptides. Palbio™ 50 RD have been shown to positively affect the intestinal health of young animals, improving digestion and feed intake to ensure productive improvements in pig, poultry, and fish. “A strategic market for Bioiberica, South Korea has some of the highest regulatory standards for animal nutrition products. As such, only the highest quality products are eligible for sale in the country,” says Joan Bassa, Business Units Director of Heparin Science, Animal Nutrition and Plant Health at Bioiberica. Dr. HB Lee, CEO of Mill Tech Co. Inc. adds: “We’re delighted to have expanded our partnership with Bioiberica, working together to bring Palbio™ to the South Korean market. We are convinced that our customers’ needs will be more than met through Palbio™, thanks to its primary effects in animals, as well as the value delivered via its assured biosecurity.” Bioiberica has new animal nutrition partnership in South Korea ment is grown sustainably using renewable energy and waste heat from another nearby factory. Next year, Volta Greentech is planning to build its first large-scale seaweed factory - Volta Factory 02 - with a planned production of 50 tonnes of the feed supplement per year. Further capital will be raised during the year for building the next factory as well as for research and development. “The 1.5 billion cows on the planet produce 5 percent of global greenhouse gas emissions, twice the amount of the world’s airplanes. We have now shown that our seaweed has the potential to reduce a large part of those emissions. The market potential and the impact we can achieve with this solution is huge,” says Volta Greentech CEO and co-founder Fredrik Åkerman. The greenhouse gas methane is 28 times more potent than CO2 at a 100-year time horizon. At COP26, more than 100 countries signed the Global Methane Pledge to reduce 30 percent of methane emissions by 2030. For many countries, reducing emissions from cattle may hold the greatest potential to reach that target.

ISSUE FOCUS 16 FEED & ADDITIVE MAGAZINE March 2022 WAYS TO IMPROVE PERFORMANCE IN DAIRY ANIMALS

ISSUE FOCUS FEED & ADDITIVE MAGAZINE March 2022 17 Copper in ruminants: From essential to toxic Valérie Kromm Animine Phytogenics: Natural additives to improve performance in dairy Delphine Lacombe Delacon Biotechnik GmbH Protected extracts from plant and seaweed to secure & boost milk production Dr. Stephanie Ladirat NUQO Feed Additives A successful lactation is a matter of preparation! Miriam Deppe Biochem How do endotoxins and rumen health influence cow performance? Claudio Campanelli EW Nutrition Enhancing early lactation in cows through plant extracts Sebastien Constantin Pancosma Milk profit: It can always be better Valentin Nenov Phileo by Lesaffre Improving fibre digestibility to unlock 3 Es in dairy production: Efficiency, economics and environmental sustainability Jac Bergman Selko Feed Additives

ISSUE FOCUS 18 FEED & ADDITIVE MAGAZINE March 2022 Improving fibre digestibility to unlock 3 Es in dairy production: EFFICIENCY, ECONOMICS AND ENVIRONMENTAL SUSTAINABILITY "Researchers at Ohio State University found that dietary changes can boost the amount of milk cows produce during the lactation period. Changing elements in the diet to improve herd feed efficiency from 1.3 kg milk/kg dry matter to 1.5, lowers the amount of dry matter cows need to consume to produce a certain amount of milk. From an income perspective, this can result in a significant increase in income over feed cost." The decisions dairy farmers make to support their herds’ performance also have a big influence on the economics and environmental footprint of their farm. Improving feed efficiency is a proven strategy for increasing milk yield, improving income, and reducing the environmental footprint of dairy farming. Simply defined, efficiency means producing more without increasing inputs. Recently, the dairy sector has turned its attention toward improving feed efficiency. Given that the cost of feed is the largest contributor to production costs, improving the digestibility of dietary fibre is a key opportunity to drive better feed efficiency. Research conducted by Trouw Nutrition and leading agricultural universities show the benefits of dietary strategies to improve fibre digestibility in dairy cows. Below are excerpts of a conversation with Jac Bergman, Global Programme Manager Ruminants at Selko Feed Additives that addresses how improving fibre digestibility can unlock keys to improved efficiency, economics, and environmental sustainability. What does feed efficiency mean in terms of dairy farming and how is this metric calculated? Viewed through the lens of dairy farming, feed efficiency can be defined as the amount of fat corrected milk yielded per unit of dry matter consumed. In the U.S. dairy cow efficiency is most often calculated by dividing the kg of Energy Corrected Milk (ECM) by the kg of Dry Matter Intake (DMI). This method of calculating feed efficiency can be adapted to fat and/ or energy with various baselines. In the U.S., 3.5% is typically the fat corrected milk measure, whereas in most European nations the standard is 4%. Other ways for defining feed efficiency, include the kg of milk solids per kg of dry matter consumed, kg of milk per hectare (for cows in pasture environments), kg of nitrogen per kg of nitrogen consumed Jac Bergman DVM, Global Programme Manager Ruminants Selko Feed Additives This conversation is based on a webinar broadcasted in collaboration with Dr. Mike Hutjens from the University of Illinois.

ISSUE FOCUS FEED & ADDITIVE MAGAZINE March 2022 19 and kg of milk solids per unit of carbon dioxide output or greenhouse gas generated. Of course, herds are comprised of individual cows and when calculating feed efficiency for a dairy herd, there can be different efficiency values for cows of different ages or in different lactation stages, so these herd characteristics should be considered. How much can dietary changes improve feed efficiency? Researchers at Ohio State University found dietary changes can boost the amount of milk cows produce during the lactation period. Changing elements in the diet to improve herd feed efficiency from 1.3 kg milk/kg dry matter to 1.5, lowers the amount of dry matter cows need to consume to produce a certain amount of milk. From an income perspective, this can result in a significant increase in income over feed cost. As the feed efficiency of lactating cows can vary from <1.3 to >2.0, both income over feed costs and the environmental footprint can vary widely between dairy farms. What are some factors influencing dairy cows’ feed efficiency? A long list of factors can influence efficiency, but the four main ones are: ingredient digestibility which relates to feed quality, a cow’s days in milk as herds can become less efficient during the second half of lactation, somatic cell count because poor udder health can reduce milk yields and, rumen acidosis which affects digestion and impedes feed passage. Among these factors, ingredient digestibility stands out as an opportunity to influence rumen performance and subsequently economics and environmental stewardship. Even the best breeding and management practices will not deliver the benefits if a cow’s rumen function is not operating at an optimal level. Here is where dietary tweaking can help. Smart minerals, smart nutrition, smart decision Increasing feed efficiency of dairy cows means more milk is being produced out of the same amount of feed. For optimal performance, cows need a healthy rumen that digests fibre efficiently. Peer reviewed research shows that completely replacing sulphates with IntelliBond improves fibre digestibility and can increase milk production. healthylife.trouwnutrition.com Ask your nutritionist about the benefits of switching to a diet 100% free from sulphate trace minerals At last a trace mineral source that improves digestibility!

ISSUE FOCUS 20 FEED & ADDITIVE MAGAZINE March 2022 How can dietary tweaks support feed efficiency? More digestible feeds release more energy and protein to the animal which supports better feed efficiency. Improved understanding of the role that dietary, metabolizable protein plays for lactating dairy cows has established a relationship between protein in the diet and milk yield. When considering protein in the diet certain protein elements are especially valuable for supporting ruminal microbes. Ammonia, amino acids, and peptides (short chains of amino acid) are important. Sources of these acids include rumen degraded microbial protein, rumen undegraded protein and rumen protected amino acids – a more expensive option. While several amino acids are considered essential, they are not provided at required levels within most feed ingredients. Typically, a feed ingredient will have some, but not all of the amino acids. Rumen-protected amino acids are costly but potential benefits can include an increase in milk protein levels, an increase in overall milk yield during lactation and higher milk fat levels. Milk urea nitrogen is a measure of the cow’s ability to capture nitrogen originating from ongoing microbial protein synthesis in the rumen. The process measures the amount of ammonia processed by the liver, that becomes trapped in the milk. If levels are too high, the result can be reduced nitrogen efficiency and both fertility and rumen function may start to decline. Trace minerals provide an interesting option for supporting feed digestibility. Careful attention to the trace minerals included in a cow’s diet can have a big impact on rumen function and thus the digestibility of the dairy cow’s diet. How does trace mineral management support rumen performance in dairy cows? Trace minerals are an essential part of ruminant rations. As the basal diet does not supply these essential nutrients at adequate levels, they must be supplemented. But not all trace minerals sources are the same and the source of trace mineral has been shown to play a big role in how much of the mineral is available for use by the animal. Sulphate trace minerals have been used as a source of trace mineral supplementation in ruminant rations for the past 75 years. They are also commonly used in footbaths because they readily release trace metal ions known to be antimicrobial. While this mode of action is good in footbaths where free metal ions can detach and attack hoof related infections, such properties are highly undesirable in the rumen. Inside the rumen, sulphate salts are highly soluble upon contact with moisture, followed by their release of high concentrations of free metal ions that can now attack rumen, fiber digesting bacteria. Additionally, as trace minerals break down following contact with moisture, they can interact with other essential ingredients and additives in the feed, potentially reducing the value of costly feed ingredients. What can farmers do to protect their herds and their business from the challenges posed by sulphates? Choosing a trace mineral source that is not soluble is a good first step in preventing the harm posed by sulphate minerals. Hydroxy forms of trace minerals are largely insoluble at a pH of 4 or higher. As the rumen pH of dairy cattle is typically in the range of 6 to 6.5, hydroxy trace minerals are essentially insoluble (inert) within the rumen. In the abomasum, where the pH is usually below 3, Intel-

ISSUE FOCUS FEED & ADDITIVE MAGAZINE March 2022 21 liBond crystals dissociate layer by layer, resulting in a gradual and sustained release of trace metal ions in the cow’s duodenum. This strategic release supports the higher bioavailability of hydroxy trace minerals by the animal to support performance. Can the choice of trace mineral support other desirable activity in the rumen? As rumen fermentation improves, the production of volatile fatty acids which serve as an important source of energy increase. A trial including two group of animals compared animals fed sulphate trace minerals to a group receiving IntelliBond. Feeding IntelliBond resulted in an increase of volatile fatty acid production in the rumen of 11%, resulting in an increase in propionate production of 15% compared to animals receiving the control diet. Another trial conducted at Cornell University in New York State found that cows fed hydroxy trace minerals had increased milk production in early lactation compared to cows fed sulphate trace minerals. And from an efficiency perspective, cows fed hydroxy trace minerals reached peak production sooner and produced 3.5 liters more milk at the peak of lactation. The dynamics of lactation persistency in dairy cows indicate that 1 kg of extra milk at peak lactation is equal to 200 kg of extra milk per lactation. Other studies conducted with IntelliBond have shown a significant increase in fat corrected milk. Beyond improving efficiency and the environment, how can strategies to support fiber digestibility support producers’ economics? Feed efficiency is a key driver of dairy farm profitability. By tweaking the diet to improve fibre digestibility – like replacing sulphate trace minerals with hydroxy trace minerals - cows can produce more milk from the same amount of feed. Depending on the milk price, the incremental cost to supplement a cow with IntelliBond throughout her entire production cycle can be earned back within 3 to 4 weeks, resulting in a strong return on investment and a significant improvement of income over feeding costs on the dairy farm. References available upon request Figure 1: Sulphate sources of trace minerals were shown to be significantly more soluble in the rumen of cattle than IntelliBond[1].

ISSUE FOCUS 22 FEED & ADDITIVE MAGAZINE March 2022 How can hydroxy trace minerals improve fibre digestibility? Microflora in the rumen play an essential role in digesting dietary fibre. However, the soluble nature of sulphate minerals can allow oxidative salts to dissociate and reduce microflora’s ability to digest fibre. Multiple peer-reviewed studies have shown that completely replacing sulphate trace minerals with IntelliBond hydroxy trace minerals resulted in a significant improvement of fibre digestibility. A meta-analysis of 14 studies carried out with IntelliBond hydroxy trace minerals showed an average improvement of NDF digestibility of 1.7%. Prior University research conducted with dairy cows has shown that each one-point difference in NDF digestibility can represent an extra .25 to .3 kg of daily Energy Corrected milk production. In general, how do you place a value on a high efficiency cow? Research from cows tracked at five commercial farms provides some good data to support both the economics and environmental benefits of efficiency. Cows were grouped in quadrants and researchers examined the performance of the top performing 25% and least efficient 25%. The highly efficient cows generated about 49% more profit depending on input costs and milk price. Cows in the least efficient group tended to eat more dry matter and concentrate compared to their highly efficient counterparts – yet they produced abut 10 kg less milk per day. And from an environmental perspective, cows with high feed efficiency have been found to generate less methane and produce less manure compared to less efficient cows. The FAO reports that livestock production is responsible for 14.5% of all global greenhouse emissions, of which the dairy sector accounts for about 2.2% of greenhouse gas emissions. Thus, improving dairy cows’ productivity can support efforts to reduce methane release. Figure 2: Percentage improvement of fibre digestibility in 14 studies in carried out in cattle on different diets. A positive effect on fibre digestibility was found in 12 out of 14 studies. About Jac Bergman, DVM Graduated from the Veterinary Faculty in Utrecht, the Netherlands in 1990. Worked for 4 years in private practice and the Ambulatory clinic of the Utrecht Veterinary Faculty. Joined Intervet Animal Health in 1994 and worked there for 20 years in various roles. Currently working with Trouw Nutrition as Programme Manager Ruminant Feed Additives.

ISSUE FOCUS FEED & ADDITIVE MAGAZINE March 2022 23 INTRODUCING HIGHEST CHOLINE CONCENTRATION IN THE INDUSTRY 1, 3 Zenobi et al., J Dairy Sci. 101:1088 (2018) 2 Lima et al., The Veterinary Journal. 193:140-145 (2012). All trademarks are property of Balchem Corporation © 2021 Balchem Corporation. Balchem Italia Srl Via del Porto Snc 28040 Marano Ticino (NO) Italy Telephone +39 0321 9791 Fax +39 0321 979249 E-mail [email protected] Website BalchemANH.com To learn more, contact your local Balchem representative or distributor. Combining advanced core design with industry- leading encapsulation technology, Balchem delivers the most unique rumen-protected choline product on the market today. • Most researched base choline product in the industry • Highest payload while eliminating the need for a carrier • Durable and feed stable • Reduced carbon footprint • Non-GMO • European manufacturing Highest Choline Concentration in the Industry The ReaShure line is proven to deliver results throughout an animal’s life. • 2,10 kg of milk per day improvement, or 640,50 kg more milk over the full lactation.1 • Proven reduction in metabolic disorders including ketosis, displaced abomasum and subclinical milk fever.2 • In utero impact on the calf, leading to growth and health improvements.3 Advanced Technology, Proven Results

ISSUE FOCUS 24 FEED & ADDITIVE MAGAZINE March 2022 MILK PROFIT: IT CAN ALWAYS BE BETTER Even though global demand for milk is rising, along with a positive outlook for global milk prices, the margins for dairy farmers remain tight. Increasing feed efficiency seems to be the holy grail to improve milk production, fertility and the bottom line for dairies. And there is a dedicated nutritional program doing just that. Demand for dairy has remained strong through 2020 and will continue to be the key driver in dairy markets through 2021 and 2022, impacting higher farmgate milk prices in most dairy regions in the world. In Europe for example, the average European milk price in April this year reached 35.60 Euro per 100kg, an increase of 6% compared to the same period in 2020 (source: Milk Market Observatory). At the same time, dairy farmers are confronted with ever rising feed prices, higher production costs for milk and challenges such as heat stress and fertility issues that might stand in the way of reaching the desired high milk outputs. Not all things can be controlled, but what we feed our cows can be steered. UNDERUSED POTENTIAL OF THE RUMEN All farmers want to get as much out of 1kg feed. But increasing feed efficiency depends on a pallet of factors, including the quality of feed ingredients, forages and silage in terms of digestibility and nutrient bioavailability. But also feed availability (can the cow actually access the feed), dry matter intake, overall farm management and animal health and welfare are key factors to reach high feed efficiency levels. The rumen plays a crucial role in feed efficiency and can perform at its best when the diet is well balanced and contains enough fibre content amongst others. Better rumen function contributes to better digestion of the feed and hence more available energy is available for important things, all related to milk profit. • More energy to spend on milk production • More energy available to be fit for breeding • More energy available to deal with challenges such as heat stress. The rumen is, however, often challenged in high-producing dairy herds, due to high energy/ concentrated diets, not enough fibre content, overcrowding or climate conditions. This can lead to a malfunctioning rumen and associated problems such as rumen acidosis. By better taking care of the rumen, the rumen potential is therefore met, directly reducing nutrients loss while improving both milk yield and quality in the tank. THE PROGRAM MILK PROFIT New nutritional solutions are available to dairy farmers that enhance rumen health, the first step to better performance and breeding success. To achieve this, we have to look at the total lactation period of the cow, from the transition period all the way through the end of the lactation. This is because cows in each Valentin Nenov Global Ruminant Manager Phileo by Lesaffre

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