Alternative Proteins Magazine - October 2024 Issue 4

ARTICLE 52 Alternative Proteins Magazine October 2024 Here is a brief on the applications of Silkworm derived ingredients in animal feeds & pet foods: Applications in Livestock Feed: 1. Poultry Feed: Silkworm pupae meal can be used as a protein supplement in poultry diets, improving growth performance and feed efficiency. 2. Aquaculture: It is also suitable for fish feed, enhancing growth rates and overall health of fish. 3. Swine and Ruminant Feed: Inclusion of silkworm pupae meal in swine and cattle diets can improve protein intake and promote better growth. It is also proven that in vitro rumen methane production goes down by 15-30% with an inclusion of 2-4% of Silkworm Pupae Oil, a promising way to not only provide necessary lipids in the diet but also solve for GHG emissions. Applications in Pet Food: 1. Dog and Cat Food: Silkworms can be processed into pet food, providing a novel protein source that is hypoallergenic and highly digestible. 2. Exotic Pets: For reptiles, birds, and other exotic pets, silkworms are a natural and nutritious food option. Table 1-2-3 are comparative analyses of the typical proximate compositions, Amino Acid profiles, Phospholipids & Sterols of Silkworm Pupae products in comparison to other conventional sources. APPLICATIONS OF SILKWORM PUPAE OIL IN AQUA FEED FORMULATIONS Silkworm Pupae Oil emits a distinctive and potent aroma, making it an effective chemoattractant to enhance feed palatability and attractability. The emergence of white spot syndrome (WSS) poses a significant challenge to crustacean aquaculture, resulting in substantial economic losses. Beta-sitosterol, identified as a potent inhibitor of white spot syndrome virus (WSSV), shows promise as an effective agent against WSS outbreaks in crustacean aquaculture1. For optimal growth of fish and crustacean larvae, diets typically require 1–3% phosphatidylcholine + phosphatidylinositol (PC + PI) of the diet's dry weight2. Alpha Linolenic Acid (ALA), constituting over 35% of Omega-3 fatty acids in Silkworm Pupae Oil, significantly influences the growth performance of fish like Coho Salmon (Oncorhynchus kisutch), impacting hepatic lipid metabolism enzymes and muscle fatty acid composition3. Research on White Legged Shrimps indicates that dietary supplementation with Linolenic Acid upregulates immune genes (peritrophin-44-like protein, lysozyme, and cathepsin C) and enhances antioxidant enzyme activity (superoxide dismutase Test parameter Unit Silkworm Pupae – Full Fat powder Silkworm Pupae – Defatted Meal Fish Meal 60% Fish Meal 65% Squid Liver Powder Shrimp Head Meal Soya Bean Meal Source Moisture Fat Protein Ash Fibre Carbohydrate Energy Loopworm Pvt Ltd 5.6 33.6 50.1 4.9 4.1 1.7 525.8 Loopworm Pvt Ltd 5.3 13.2 70.3 5.7 4.3 1.3 419.3 Indian Fishmeal producer 7.7 9.2 62.0 20.2 1.3 0 331.5 Indian Fishmeal producer 7.9 5.0 66.7 20 0.5 0 312 Indian Squidmeal producer 5.6 7.7 49.9 6.9 4.6 25.3 369.6 Indian Soymeal producer 9.9 0.9 54.5 7.2 6.3 21.2 310.5 Indian Jawla meal producer 6.9 3.5 55.6 28.7 3.0 2.3 263.6 g/100g g/100g g/100g g/100g g/100g g/100g kcal/100g Table 1

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