F&A Alternative Proteins Edition

April 2023 Evelien van Donselaar, Selko Converting residual streams into protein accurately and reliably Alex Diana, Innovafeed The impact of the insect industry on sustainable protein production Robert Mansfield, Deep Branch Scaling gas fermentation technology to market-size animal feed protein www.feedandadditive.com TRENDING ISSUE by Feed Additive Magazine Alternative Proteins

The rate of increase in the world's population continues to be a cause for concern because feeding this growing population with what nature offers us seems challenging in today's conditions. That's why the experts of the agricultural industry focus on sustainable alternative resources and the efficient use of existing resources. Proteins, which have an important place in human and animal nutrition, are also one of the areas on which intensive studies continue. Alternative plant and cell-based protein options, insect-based protein sources and protein substitutes from algae, yeast, fungi or bacteria are attracting considerable attention as alternative protein sources used directly or indirectly (food and feed substitutions) in both human and animal nutrition. However, feeding the growing population is not the only reason alternative protein sources have attracted so much attention. When we add the fact of environmental concerns and sustainability to all these, it becomes inevitable to evaluate alternatives better. As Feed & Additive Magazine team, we have prepared a special issue this month to draw attention to alternative protein sources, the value of which is increasing day by day in animal nutrition. The content of this first special issue consists mainly of articles on insects and insect-based proteins. The leading representatives of the insect industry share with our readers the valuable information they have gained through their practices and research studies in the industry. We also tried to cover how we can turn existing resources and food products that are treated as waste into protein sources or how we can scale protein production with technology through our exclusive interviews. We hope this is a useful issue that you will enjoy reading. Hope to meet you again in our following special issues to discuss other alternative protein sources in more detail in the near future… Enjoy your reading… Publisher Muhammed Akatay [email protected] Responsible Manager Özlem Buyruk Akatay [email protected] Editor-in-Chief Derya Yıldız [email protected] Editors Bahadır Büyükkılıç Burak Varol [email protected] Design Alper Kandemir [email protected] Web Design Caner Cem Pulat [email protected] Proofreading Lisa Atakul [email protected] Circulation and Data Responsible Amine Şengün [email protected] Ataturk St. Metropol Istanbul Ste. Block C1, No:376 Atasehir, Istanbul/TURKEY Phone: +90 850 220 5064 E-Mail: [email protected] Web: www.feedandadditive.com Publication Type: Periodic (Monthly) EDITOR Alternative sources for sustainability in nutrition Derya YILDIZ © Three Plus Media | All rights reserved. Reproduction, in whole or in part, is prohibited without the written permission of the publisher. *DISCLAIMER: All views and ideas expressed in articles published in the magazine reflect the point of views of the author(s), not that of the Editor-in-Chief, Editors, Editorial Board or Publisher of Feed & Additive Magazine. The Editor-in-Chief, Editors, Editorial Board and Publisher assume no responsibility or liability in such cases. The publisher does not give any warranty and representation to the accuracy or completeness of the contents and data published in the magazine. All responsibility of the contents, articles and information published in the magazine belongs to the authors. The publisher and the authors cannot be held responsible for any damages resulting from the use of the whole or part of the content of the magazine. feedandadditive Dear Readers, Alternative Proteins +

Replacing proteins and fats with more sustainable alternatives, while maintaining palatability and performance, is a major challenge. The answer lies in insect ingredients. Insects are a natural source of nutrients for chickens, piglets and fish. Protix is the world’s only industrial-scale supplier of insect ingredients. The company harnesses the black soldier fly’s amazing ability to upcycle food waste into high-quality ingredients that are good for animals and good for the planet. Your partner for insect ingredients Learn more: www.protix.eu - [email protected]

Protix launches fresh insect meat PureeX® for pet food 6 Anpario moves from fish oil to microalgae with Optomega Algae 6 ADM and Believer Meats brings expertise together to cultivated meat 9 Nutrition Technologies exports insect meal and oil to EU and UK 10 Entobel expands its operations in Vietnam 7 Aleph Farms launches new product brand Aleph Cuts 9 FeedKind protein wins FDA GRAS status for use in aquaculture 11 Mosa Meat and Nutreco work together to reduce cost of cell feed 8 SIIG to invest in sustainable protein company Unibio 8 CULT Food Science and Umami Meats supply cultivated red snapper for pet food 10 NEWS CON T E N T S INTERVIEWS CONVERTING RESIDUAL STREAMS INTO PROTEIN ACCURATELY AND RELIABLY Evelien van Donselaar, Selko Feed Additives 12 “THE USE OF ALTERNATIVE PROTEINS HAS MOVED FROM A TREND TO REALITY” Dr. Christian Kling, ANDRITZ Separation GmbH 18


NEWS 6 F&A Alternative Proteins Edition April 2023 Anpario’s Optomega product range is renowned for helping producers to deliver omega-3 enriched milk, meat, and eggs, as well as supporting livestock fertility and other crucial biological functions. To date, Anpario has focused on utilizing fish oils to develop these products, however, the company has announced a move to an alternative, sustainable omega-3 source – microalgae. “To ensure we are providing customers with the best quality products, Anpario has been focussing on the development of new effective and sustainable omega-3 alternatives,” explained Andrew Jackson, Anpario’s Commercial Director. “Due to dietary restrictions for farmed fish, there has been a reduction seen in the levels of omega-3 fatty acids, specifically EPA and DHA, present in the final oils. When Anpario moves from fish oil to microalgae with Optomega Algae New fresh insect meat PureeX has been launched by insect ingredients company Protix. According to the company, the delicious product adds new dimensions of freshness to pet food. Natural animal feed additives manufacturer Anpario announced a move from fish oil sources to microalgae for sustainable omega-3 sources. In response to demand for fresh, healthy and sustainable pet food, leading insect ingredients company Protix launched fresh PureeX®, an appetising fresh insect meat. According to the company, this new variant on frozen PureeX offers pet food manufacturers and their customers all the health benefits of proteins and lipids from insects, while also lowering the ‘pawprint’ of pet food. For this innovative product, processing is simple and rapid, resulting in an authentic and honest ingredient that is minimally processed and maximally nutritious. In two lab studies with PureeX, the company found a stronger antioxidant activity compared to commonly-used pet food ingredients. In the competitive pet market where consumers want to keep their four-legged friends healthier for longer, the higher antioxidant activity of fresh PureeX delivers a potential added benefit. Thanks to straightforward rapid processing with a minimum amount of steps, fresh PureeX is an ingredient which is low in biogenic amines, a commonly used measure of freshness. It has a low footprint, just like its counterpart frozen PureeX. In a recently published DIL sustainability report, frozen PureeX scored 24x better in terms of global warming than poultry meat (0.439 kg. CO2 equivalent versus 10.33 kg. CO2 equivalent) and similar data are expected for fresh PureeX. Read more>> Protix launches fresh insect meat PureeX® for pet food

NEWS F&A Alternative Proteins Edition April 2023 7 combined with significant market price movement, seeking alternative omega-3 sources and revaluating the product range ensures both value and quality are maintained for customers”. The last few years have seen Anpario develop innovative Optomega Algae products, which provide a rich, high-quality source of the omega-3 fatty acid DHA. Containing oil derived from sustainably sourced microalgae blended onto a unique carrier system for a free-flowing, easyto-use powder product with a superior shelf life. According to the company’s statement, the microalgae sources used by Anpario are produced by fermentation. This process is highly sustainable, utilizing renewable sugarcane production with waste cane stocks being used as an energy source to power the facility. Read more>> Entobel, one of the leading global insect-based ingredient producers, partnered with the International Finance Corporation (IFC) to scale up its operations in Vietnam. As part of a long-term collaboration agreement that includes early-stage project development support, IFC will support Entobel’s expansion in Vietnam and the region. Based in Singapore, Entobel is one of the very few players already producing insect ingredients at a commercial scale from the Black Soldier Fly-a species that provides a great source of protein-with applications in high-quality aquafeed and pet food. Entobel is currently building Asia’s largest insect plant to date in Vung Tau province, Vietnam, which will supply 10,000 metric tons of insect meal annually to local and international aquafeed and pet food-producing customers. The construction is on schedule and the first commercial sales are expected later this year. The main product of Entobel is insect meal, which is used, among other applications, as a sustainable alternative to fishmeal in the aquaculture industry. The impact of Entobel will be to replace wild-caught fish being used for fishmeal, thus helping to alleviate over-fishing. “We are impressed by Entobel’s operations in this innovative industry, which can provide a sustainable high-protein alternative to wild-caught fish used in aquafeed. Reaching scalable levels of production will help protect marine ecosystems and reduce the depletion of ocean resources. We are happy to support Entobel on this exciting and impactful journey,” said Thomas Jacobs, IFC Country Manager for Vietnam, Cambodia, and Lao PDR. Co-founders Gaetan Crielaard and Alexandre de Caters, both Belgians, moved to Vietnam in 2013 after realizing the vast potential of this new and innovative industry. Southeast Asia is a main aquaculture hub and the tropical climate is ideal to rear the Black Soldier Fly with relatively lower energy costs compared to competitors in Europe. Read more>> Entobel expands its operations in Vietnam

NEWS 8 F&A Alternative Proteins Edition April 2023 Saudi Industrial Investment Group (SIIG) agreed to invest US$70 million in sustainable protein company Unibio International PLC. The company will use the investment to improve the world’s food security. Unibio International PLC, the leading sustainable protein company, announced that the Saudi Industrial Investment Group (SIIG) has signed an agreement to invest approximately US$70 million (GBP 59 million) in Unibio. The proceeds will be deployed to enable Unibio’s vision of feeding the world’s growing population sustainably. Unibio will use the funds to roll out new global production capacity, grow operating capabilities, and accelerate innovation and commercialization. Through industrial protein production based on Unibio’s unique fermentation technology – the U-Loop® technology – it is possible to improve food security and feed the world’s growing population sustainably without the use of arable land and with low water usage. The fermentation technology mimics a process that occurs in nature every day and uses methane (e.g. natural gas or biogas) as feedstock. The production of Uniprotein® is efficient, stable, and independent of weather deviations that impact the production of traditional protein sources. Uniprotein® is approved for feed in the European Union and global registrations are in progress. Read more>> Mosa Meat, a leader in the cultivated meat space that grows beef directly from animal cells, signed a Letter of Intent with Nutreco, a global leader in animal nutrition and aqua feed, to collaborate on creating a cell feed supply chain. This announcement is a new milestone in the longstanding partnership between Mosa Meat and its investor Nutreco – jointly awarded a REACT-EU grant for their ‘Feed for Meat’ project in October 2021, which strives to lower the cost of cultivated beef while creating a robust supply chain to scale up production. Optimising cell feed has been widely considered a key challenge that needs to be overcome for the nascent cultivated meat industry to reach the commercialisation stage. The LOI between Mosa Meat and Nutreco comes after scientists at the two companies have confirmed that a basal media (cell feed) formulated with food-grade ingredients instead of pharma-grade ones performs equally well at a substantially lower cost – a critical step to further develop the cellular agriculture supply chain. Furthermore, scientists at Mosa Meat confirmed that 99.2% of the basal media by weight has been successfully replaced by food-grade components in the serum-free cell feed. “Our partnership with Nutreco represents our commitment to further develop the cellular agriculture supply chain and bring down costs,” shared Maarten Bosch, CEO of Mosa Meat. “Our scientific results are an industry first, proving that food-grade ingredients perform equivalent to pharma-grade in cell feed. This will represent a significant cost savings as we scale up production.” Read more>> SIIG to invest in sustainable protein company Unibio Mosa Meat and Nutreco work together to reduce cost of cell feed

NEWS F&A Alternative Proteins Edition April 2023 9 Aleph Farms, a food technology company designing new ways to grow quality animal products, announced the launch of its first product brand, Aleph Cuts. Under the Aleph Cuts brand, the company will market its first product, the Petit Steak, the world’s first cultivated steak anticipated to launch in Singapore and Israel later this year, pending regulatory approvals. Developed in partnership with brand and experience design agency, BOND, this branding initiative distinguishes Aleph Farms’ products and builds momentum ahead of commercialization and subsequent engagement with customers and end consumers. “With the launch of Aleph Cuts, we are introducing our product through an epicurean lens to connect people to our incredible ‘new take on steak,’ sharing what this choice means in an engaging and authentic way,” said Nicky Quinn, VP Marketing at Aleph Farms. “Iconic brands aren’t built overnight or by one person or team. We look forward to co-creating our brand over time with consumers, so we can best serve their evolving needs.” The visual identities for Aleph Farms and Aleph Cuts are part of a unified system designed to support the scale of Aleph Farms’ growing product portfolio. Read more>> Aleph Farms launches new product brand Aleph Cuts ADM and Believer Meats signed non-exclusive MOU to broaden the protein ecosystem by collaborating on development of new cultivated meat products to deliver on growing consumer demand. ADM, a global leader in sustainable nutrition, and Believer Meats, a leading pioneer of the cultivated meat industry, announced that they have signed a non-exclusive memorandum of understanding (MOU) to collaborate on new ways to propel the development and commercialization of cultivated meat products. “With a global population expected to reach 10 billion by 2050, ADM is working with partners across the value chain to meet food security and sustainability needs by expanding the protein ecosystem,” said Leticia Gonçalves, ADM’s president, Global Foods. “Cultivated meat represents an exciting evolution and yet another way to meet long-term food security needs — along with other traditional and novel sources of protein. Seven in 10 consumers are aware of cultivated meat, and with flexitarians representing more than half of consumers globally, there is an important opportunity to continue to expand the universe of protein choices. We’re proud to work with a true innovator like Believer to explore new ingredients and solutions to propel the development of these exciting products, and excited to continue to add valuable partners as we work across the industry to meet growing demand driven by the enduring trends of food security and sustainability.” Read more>> ADM and Believer Meats brings expertise together to cultivated meat

NEWS 10 F&A Alternative Proteins Edition April 2023 CULT Food Science and Umami Meats supply cultivated red snapper for pet food CULT Food Science Corp., a pioneer in the investment, development, and commercialization of cellular agriculture technologies and products, announced a partnership with Umami Meats to supply cultivated red snapper for their newly launched pet food brands. In collaboration with Umami Meats, the Company is including cell-cultivated fish in its cat treat brand, Marina Cat. Cell-cultivated fish ingredients are produced by taking a sample from a fish and then growing and scaling the cells in large bioreactors. This innovative process allows for the creation of fish-based products such as sushi, sashimi, fish balls, fillets, and now, cat treats, without the need for high-impact ocean harvesting. This will be one of the world’s first cell-cultivated fish pet products brought to market. According to the company’s statement, Marina Cat is focused on the health benefits of ocean-derived ingredients that promote longevity and immunity. It’s a modern treat for cats, exclusively using cell-based fish and marine ingredients, avoiding ocean-devastating commercial fishing practices. The availability of Marina Cat treats will be announced in the near future. Read more>> The Malaysian Ministry of Agriculture approved Nutrition Technologies to export its insect meal and oil into the EU and UK markets, making it one of a handful of companies around the world, and the first in Malaysia with this level of approval. Previously only able to access European insect-based products, this development opens the doors for the rapidly growing sustainable petfood sector in Europe to access high quality, low-energy insect-based materials. In June of 2022 insect producers in Malaysia became eligible to export insect-based material to the EU for the first time, following Malaysia’s inclusion on the list of countries approved to export insects and insect-based products to the EU, joining a small selection of countries including; Canada, Switzerland, United Kingdom, and South Korea, with permission to do so. This is considered a boon for the Malaysian economy, a major food producer which is heavily reliant on agricultural exports. Nutrition Technologies exports insect meal and oil to EU and UK

NEWS F&A Alternative Proteins Edition April 2023 11 Calysta, one of the world leaders in fermented protein, announced that its leading product, FeedKind®, has achieved generally recognized as safe (GRAS) status with the United States Food and Drug Administration (FDA). The FDA’s Center for Veterinary Medicine have granted Calysta’s FeedKind protein GRAS status for use in the US in salmonid feeds at up to 18% of the diet. In the US FeedKind will be listed under its common name, dried fermentation biomass. According to the company, the ruling means aquaculture companies in the US now have access to a nutritionally rich and non-GMO protein ingredient that both helps preserve biodiversity while addressing a growing market need for proteins for feed and food. “With so many exciting developments in US aquaculture, we are thrilled to be able to access this market. FeedKind protein is a perfect fit for the trout industry and the burgeoning recirculating aquaculture systems (RAS) industry for Atlantic salmon,” said Allan LeBlanc, VP and Aquaculture Lead at Calysta. “FeedKind is an exceptional alternative to other sources of protein, and is free from heavy metals, pesticides, and microplastics, ensuring animals are only fed the nutrients they need. Because it can be produced year-round using no arable land and without using any plant or animal ingredients, it truly is a protein without limits.” “US GRAS status was a key achievement for us, so we are very pleased with the official notice. This brings the US closer in line with existing approvals in other markets throughout the world, such as the EU, and enables us to expand the market opportunity for this revolutionary product. This is an important first step in our path toward US GRAS status for use in pet foods to complement our existing EU and UK approvals for pet food,” said Herman Sloot, Vice President of Commercial Development. Read more>> FeedKind protein wins FDA GRAS status for use in aquaculture Nutrition Technologies manufacture and supply insect protein meal, oil and frass (insect manure) to help feed the growing agriculture and feed industries around the world. Using a combination of beneficial microbes and Black Soldier Fly Larvae (BSFL) using a low-energy, zero-waste production model to grow their insects, the larvae are reared on clean and traceable agro-industrial by-products. The insect-based products are suitable for application in pet food, livestock and aquatic feed, and have a range of proven functional benefits which improve the animals’ health and growth. The insect meal is also suitable for therapeutic applications in pets that have allergies to common petfood proteins such as beef and lamb. What’s attractive to many manufacturers is the potential impact on GHG emissions that can be achieved through switching to insect-based meats in place of traditional protein sources, as approximately 25% of the GHGs associated with meat production is generated through use in petfood. Since tropically farmed insects produce a fraction of the GHG emissions of traditional meat – this enables manufacturers to make further improvements to the carbon footprint of petfood production. Read more>>

INTERVIEW 12 F&A Alternative Proteins Edition April 2023 Human or animal… There has to be a sustainable way to feed us all with what nature offers. This is exactly what experts in the agricultural industries are chasing: To sustainably feed the living things on the planet… Because reputable organizations that make predictions based on the rate of increase in the world population are united on the possibility of our future nutritional problems. Companies known for their expertise in the animal nutrition industry, such as Nutreco and its subsidiary Selko Feed Additives, have already stepped up their efforts to avoid this problem. While these companies are looking for ways to increase animal protein production in a sustainable way, they also focus on how to use existing resources more efficiently. Evelien van Donselaar, Business Manager for Food2Feed at Selko, shared with us one of the ways how animal protein production can be increased sustainably: Evaluating by-products from the food- and beverage industry correctly and reliably… The fact that humans need the same natural resources/raw materials as animals as a food source is a topic that has been talked about a lot in recent CONVERTING RESIDUAL STREAMS INTO PROTEIN ACCURATELY AND RELIABLY Evelien van Donselaar, Business Manager Food2Feed, Selko Feed Additives “Various initiatives, such as applying digital farming strategies, the development of fermentation-based and cell-based protein or incorporating insects in animal and human food are developing rapidly. All of these approaches help answer the immense question of how to provide healthy nutrients to feed all of earth's inhabitants. With Selko, the feed additive brand of Nutreco, we play our part in this big picture, by upscaling by-streams from the food- and beverage industry.”

INTERVIEW F&A Alternative Proteins Edition April 2023 13 years. What is the real meaning of this? Why is consuming the same resources considered a serious problem? This is an interesting question. A common misconception we see in the industry is the belief that humans and animals compete for the same resources. In fact, according to the 1st Feed Sustainability Charter progress report (2021) of the European Feed Manufacturers' Federation (FEFAC), 96% of the grains, soybean products or pulses are grown specifically for animal feed production and are of a quality standard that does not meet the standards required for human consumption. This tells us that it is not the cereal itself, nor its availability, that are causing friction, but rather the use of arable land. The challenge of feeding an additional two billion people in a mere 30 more years is placing increased demand on our planet’s resources. The industry is trying to find ways to produce more protein with fewer resources. We need to find a way to manage a growing population’s demand for more food with the planet’s limited land resources for a long time to come. When it comes to utilizing arable land, we need to look at opportunities to reduce the amount land required to produce cereals for animal consumption, which will allow more land to be used for food-grade cereals. This means we need to look at other sources of feed ingredients, like by-products from the food- and beverage industry, grain by-products and increasing feed efficiency (feeding lower amounts while maintaining production performance). When we think that we need more food to feed the growing world population, but our natural resources are limited, is there a way to use the available resources more efficiently? For example, what is the way to separate our resources from animals directly or indirectly? There are abundant opportunities to achieve more sustainable protein production and feed a growing population. Various initiatives, such as applying digital farming strategies, the development of fermentation-based and cell-based protein or incorporating insects in animal and human food are developing rapidly. All of these approaches help answer the immense question of how to provide healthy nutrients to feed all of earth's inhabitants. With Selko, the feed additive brand of Nutreco, we play our part in this big picture, by upscaling by-streams from the food- and beverage industry. Keeping in mind the 'food waste hierarchy' of the

INTERVIEW 14 F&A Alternative Proteins Edition April 2023 European Food Safety Authority (EFSA, figure 1), we refuse to call these products 'waste' since they contain re-usable components such as carbohydrates, fibre, protein, and other nutrients that can be used to produce animal feed. With effective and safe preservation, these products can be diverted from ending up in bio-fuel production or land fill and serve as high quality feed ingredients. Food and beverage production takes place all over the world and is not bound to one specific location. This means that by-products can often be sourced locally, reducing the need for long distance transport or energy consuming drying processes. For example, in the Netherlands, 5.4 million tons of liquid and moist by-products were used by home mixing farms in 2021, which would be roughly equal to 1.25 million tons of dry feed materials (Circular Feed Association). However, in other parts of the world, by-products are not utilised at all and continue to end up as landfill. Our aim is to connect the food- and beverage industry with the animal industry, creating new, powerful partnerships and resulting in long-lasting shared efforts that improve sustainability. Some of the resources that are extremely valuable for both human and animal nutrition are wasted for various reasons. Today, a new recovery model called circular economy is on the agenda in order to eliminate this waste. What is circular economy? What is the place and meaning of the circular economy in the food and feed sector? The term 'circular economy' can be explained in many ways. There is no fixed definition or formula on how to achieve it. Basically, the industry is moving from a traditional linear economy, based on a 'take-make-dispose' approach, where residues are not used, towards a more circular economy, where companies are aware of the losses that occur along the production chain and try to find ways to utilise them. This so-called circular approach emphasises reduce, reuse, and recycle (or upcycle) principles. Today, in our feed and food industry, we might even call it 'infinite economy' as by-streams from one process can supply animal protein producers who in turn supply by-products to the food and beverage industry in a never-ending cycle (figure 2). Our role within Selko is to connect the dots within the infinity cycle and to effectively preserve nutritious by-products to ensure their value within the food-to-feed chain. Figure 1. Food waste hierarchy (Source: European Commission)

INTERVIEW F&A Alternative Proteins Edition April 2023 15 What waste can the animal nutrition industry consider as an alternative feed or feed ingredient within the scope of the circular economy model? This question does not have a simple answer. If we were to list all possible by-stream candidates that can act as feed ingredients, we would need many more pages. As a rule of thumb, all pure by-products containing substantial levels of carbohydrates, protein or fibre can be suitable candidates. Some examples of companies we have partnered with over the years are (global) beer brewers, utilising spent yeast and spent grains, pulses and grain processors, utilising pea cream and starch-rich by streams, animal slaughter facilities, utilising process water, and the dairy industry, utilising whey and permeate. In all these examples, preserving ingredients in a cost-effective and safe way has been on a caseby-case basis relying on customised inclusion and application of Selko's Revalet product range. The best results are achieved through applying products at the right time and with the best possible dispersion. Selko offers state-of-the-art dosing services that allow for safe, accurate and reliable application of products. Considering that most of the wastes are foods that start to rot and spoil, we cannot ignore the fact that they are contaminated by different microorganisms. Therefore, how can we protect and transform these wastes in a way that does not pose a risk to human and animal health? It is indeed correct that by-products and other residual streams are exposed to microbial challenges when left untouched. Especially in liquid or moist products we see challenges with yeast or Enterobacteria, and in some cases even both. Within hours, yeast and microbes can significantly decrease nutritional value and produce vast amounts of CO2, making them dangerous to process or store and rendering them unfit for animal feeding. Next to microbial pressure, there is also an increased risk of mycotoxins, especially in grain by-products like DDGS, maize gluten meal or rice by-products. Most mycotoxins are present on the outer parts of the kernel. As the core is removed, the overall mycotoxin concentration in these by-products can reach levels that can harm animal health and performance. Figure 2. Effective preservation of by-products is key when working in an infinite economy.

INTERVIEW 16 F&A Alternative Proteins Edition April 2023 Selko offers various solutions to preserve moist and liquid by-products and to mitigate mycotoxins in grain by-products. When it comes to moist and liquid by-products, Revalet has proven to be a cost-effective way to deactivate yeast and preserve valuable by-products (figure 3). As there is no silver bullet solution, each customer situation is thoroughly investigated, and inclusion rates are determined on a case-by-case basis. As Selko Feed Additives, what are the solutions you offer for more efficient use of resources and circular economy model? In over four decades of bringing effective feed additive solutions to the market, we learned that simply applying a product is not enough to truly make a difference in today's fast-changing economy. To make steps towards an infinite economy, we need to act as a connector between industries and look beyond our own solutions alone. This is where I believe Selko differentiates our solutions. Being part of Nutreco allows us to utilise our global network and connect both global and local players in the food and feed industry. With our nutritional know-how, global presence, and customer-driven services, we bring effective solutions that help produce safe, nutritious feed. I am convinced that all our initiatives, whether it is applying our Selko solutions, investing in fermentation- and cell-based proteins, implementing digital farming models and solutions or providing advice on nutrition and good farming practices, we all work towards our shared passion of feeding the future. About Evelien van Donselaar Evelien van Donselaar, Business Manager Food2Feed at Selko, focuses on providing practical solutions for- and creating connections within- the food and feed industry. She is responsible for Selko Revalet®, a product portfolio that reduces microbial challenges from the source, preserving nutritional value of by-products. Her animal healthcare background and extensive experience in working for innovative companies focusing on production of alternative proteins, as well as a passion for upcycling by-products, allow her to think out of the box and support the company’s purpose of Feeding the Future. Figure 3. Dose response testing confirmed the most cost-effective solution. After day 7 the control sample was deemed unfit for feeding due to pungent smell, indicating complete microbial wastage. Therefore, it was taken out of the study.

INTERVIEW F&A Alternative Proteins Edition April 2023 17 Sustainability goals and raw material scarcity mean by-products from the food and beverage industry is now seen as a viable, nutritious source of energy for animals. Trouw Nutrition offers solutions to help the industry use resources more efficiently and move towards a circular, more sustainable economy. THE BENEFITS FOOD2FEED QUALITY Reduce landfill Upcycle by-products Maintain nutritional value Sustainable alternatives that make a little go a long way Learn more, visit www.selko.com

INTERVIEW 18 F&A Alternative Proteins Edition April 2023 In these days when feeding the growing world population is becoming a major challenge, there is an increasing interest in sustainable alternative resources and efficient use of existing resources. Andritz is one of the companies that has increased its efforts towards these resources. The company focuses heavily on plant-based protein sources, insect proteins and side streams that are considered waste. Technologies for the processing of these resources and their use in human and animal nutrition are realized with Andritz's engineering expertise. Dr. Christian Kling, Product Home Manager Alternative Protein Solutions at ANDRITZ Separation GmbH, highlights both the importance of alternative proteins in human and animal nutrition, and Andritz's solutions for processing these sources and the role of technology in scaling alternative protein production. Mr. Kling, we have to talk more and more every day about how to feed the growing world population. How much do you think alternative proteins, which are highlighted as alternatives or supplements to animal proteins and described as promising, can contribute to feeding the world population? Feeding the growing global population currently is a core challenge and it will be in future. Most important is to find sustainable ways to increase protein availability. Using alternative proteins to produce replacements for meat is a well-known example. “THE USE OF ALTERNATIVE PROTEINS HAS MOVED FROM A TREND TO REALITY” Dr. Christian Kling, Product Home Manager Alternative Protein Solutions at ANDRITZ Separation GmbH “At ANDRITZ, we focus on plant-based proteins, insects and side streams, esp. animal residues. We partner with our customers to design and engineer tailored solutions that meet their needs, depending on the raw material, conditions on the customer site, and the desired quality of the end-product. With more than 170 years engineering experience, multiple delivered solutions in feed and food and the broadest portfolio in industry, we are up for every challenge.”

INTERVIEW F&A Alternative Proteins Edition April 2023 19 This already is a large market with a huge growth potential. Still, this does not meet the demand of consumers looking for meat in their diet, esp. in the areas with high population growth rates where the consumption per capita is lower compared to the Western world. This drives a strong demand for sustainable alternative protein sources. ANDRITZ focuses on plant-based sources (i.e. peas and pulses or oilseed crops), insect proteins and side streams (i.e. animal residues or spent grains) as most relevant and impactful. We are working on more efficient and sustainable technologies, that deliver more nutritious and functional proteins, which are needed to meet the demand in feed and food industry. Alternative proteins cover many different protein groups, from insect-based proteins to microalgae, cell-based meats to plant-based proteins. Which of these groups do you think will achieve a faster development and growth in the coming years? Plant based proteins are already available in feed and food, esp. pea and soy proteins on different levels of maturity. More differentiating sources will come to the market, like fava, chickpea, hemp, or canola. Using insects as a source for proteins is a relatively recent development in feed and pet food; and we still see a certain reluctance from consumers. Nevertheless, insects are a great opportunity to valorize side streams and upcycle these to proteins, thus allowing to grow the protein availability without the need for additional resources. We see a bright future for insect protein. Cultured / cell-based meat has a very high potential as well, despite regulatory approval often still pending at the moment, the production processes are at their infancy, still extremely expensive as pharma-grade raw materials are needed. Reducing the cost of production is needed. At the same time, the supply of raw materials and fermenters is not there yet. And finally, broad consumer acceptance and improved sustainability footprint on industrial scale needs to be validated. For ANDRITZ, cellbased meats provide a high future potential on the mid- to long-term. The use of alternative protein and circular economy concepts in the same sentence is increasing day by day. Especially when it comes to by-products and waste products… What is the place of the circular economy in the alternative proteins market? Considering the global protein resources, we need to utilize these resources more efficiently. Therefore, directly utilizing side streams (e.g. spent grains or animal residues) or indirectly (i.e. as feed to grow insects) are great opportunities to increase protein availability and revenue streams. As ANDRITZ, you have various solutions for different types of alternative protein (e.g. pea-based protein, food waste, insect processing, etc.). Can

INTERVIEW 20 F&A Alternative Proteins Edition April 2023 you tell us about them? At what point does ANDRITZ take place in the alternative protein market, what does it do and what solutions does it offer? At ANDRITZ, we focus on plant-based proteins, insects and side streams, esp. animal residues. We partner with our customers to design and engineer tailored solutions that meet their needs, depending on the raw material, conditions on the customer site, and the desired quality of the end-product. With more than 170 years engineering experience, multiple delivered solutions in feed and food and the broadest portfolio in industry, we are up for every challenge. To ensure a smooth process of scaling up production to an industrial scale, ANDRITZ is building a test center in Waddinxveen (NL) to develop, refine, validate, and scale protein processes. As separation experts, we continuously drive the next generation of extraction processes, being more efficient, milder, and delivering more valuable proteins (i.e. better tasting, more nutritious and higher functionality / conversion rate). As extrusion experts, we can also directly transform the protein powders to meat alternatives and in doing so, create higher customer value. Building upon our automation resources and our global service network, we remain a partner to our customers also after plant installation, ensuring uptime and increased performance. What can you say about your expectations and targets for the alternative proteins market? For example: What is the place of alternative proteins in your future plans? ANDRITZ sees, that the use of alternative proteins has moved from a trend to reality. Consequently, we have and will further invest in this business, to be the partner of choice for alternative protein producers, from conception of ideas to complete production processes, keeping them running during their lifetime. In your opinion, what is the place and importance of technology in increasing the production capacity in the alternative protein market and in gaining a real commercial scale for the industry? Sustainable scaling of production is the key to success for this industry and there is no single process technology that can be applied for all raw materials. Thus, the deciding for the right technology is key. With the industries’ broadest portfolio and the ability to scale and validate in our new test center in Waddinxveen (NL), ANDRITZ is set up to deliver the right solutions for all our customers, knowing they will always be tailored to their needs. We can build on more than 170 years of engineering experience. Our automation competences and constant research and development of new technologies help us to deliver innovative and efficient solutions. Equally important is the need for milder, but still economic, processes to preserve the nutrition and functionality of proteins. A great example here is the recently developed and launched Turbex, a novel unit operation utilizing the power of cavitation to achieve fast, mild, and efficient extraction. This can also be tested in our new test center in Waddinxveen (NL). As a company that makes investments in this field, we are pretty sure that you follow the current assets, investments and general developments in the industry closely. Based on your observations here, what kind of development do you estimate the market will show in the coming years? Most alternative proteins have overcome the peak of inflated expectations (see Gartner Hype Cycle) and now it is time to deliver on scale. At this point, a partner with scaling and execution competence is needed. ANDRITZ fills this gap. We strongly believe in the growth of alternative proteins beyond the level of feed and food markets. They are an essential component to meet the demands of a growing population.

INTERVIEW F&A Alternative Proteins Edition April 2023 21 andritz.com Got a new idea for extracting proteins? Or turning side streams into revenue streams? Brilliant. We are up for the challenge! But moving from lab idea to full-scale production can be challenging. With a history of 170 years, we have been implementing solutions for many industries including food, feed and pet food and we can help you speed up commercialization and bridge the gap. With the industry’s broadest portfolio – from milling and conditioning, dewatering and drying, extrusion and pelleting, to process automation and service – ANDRITZ can tailor just the right solution for you. Sounds interesting? Let’s scale up your production together. INFINITE POSSIBILITIES FOR ALTERNATIVE PROTEIN PRODUCTION ONE PARTNER TO HELP YOU NAVIGATE THE WAY

ARTICLE 22 F&A Alternative Proteins Edition April 2023 The agri-food sector is constantly evolving. Alternative foods and farming systems are gradually taking the lead. In particular, insect-based products have come forward as one of these new food sources, specifically in the petfood and animal feed sectors. According to the International Platform of Insects for Food and Feed (IPIFF)1, the 1 billion euros invested since its establishment is expected to triple by 20252. With a significant growth ascertained in the last few years, the industry has been shaped with rigor and structure by the manufacturers, hand in hand with researchers and the competent public authorities, to guarantee high-quality products and compliance with the regulations in play. Ÿnsect, as the world leader in the mealworm farming and manufacturing sectors, not only abides by these requirements, but ensures they are met with the highest standards available on the market. With three farms located in Europe (in France and in the Netherlands) equipped with high-end technology, the company is shaping the future of insect farming and processing. THE PETFOOD INSECT INDUSTRY BOUND BY RULES AND REGULATIONS In Europe, the petfood industry – and more generally the food and feed industry – is highly regulated. Petfood operators have to comply with the European “Food and feed law” which guarantees the safety of food and feed chains and is amongst the most stringent in the world. The mealworm industry makes no exception to the rule. Coercive requirements regarding risk analysis, traceability, good hygiene practices, etc. notably set criteria that guarantee safety of the mealworm-based petfood products placed on the market. Compliance with these requirements is enforced by competent national authorities. As such, risks linked to the potential presence of contaminants such as pathogens (microbiological), heavy metals, pesticides and toxins are closely monitored and controlled. Both as an insect farmer and manufacturer of mealworm-based products, Ÿnsect has implemented, applied, and integrated - according to the legislation With the growing demand for insect-based ingredients, particularly in the petfood and animal feed sector, it is ever more important to showcase product reliability and quality. Subjected to the requirements of the food and feed industry, Ÿnsect goes above and beyond to ensure compliance with relevant regulations and petfood market standards but also to push the boundaries of innovation regarding mealworm housing and breeding. ENSURING SAFETY OF MEALWORM PRODUCTS Benjamin Armenjon, PhD General Manager Ÿnsect

ARTICLE F&A Alternative Proteins Edition April 2023 23 - the required hygiene practices, traceability systems, and recall requisites throughout its farming systems and production lines. Whenever applicable, hazard analysis (microbiological, chemical, physical) is carried out in accordance with the European HACCP (Hazard Analysis and Critical Control Point) principles - or equivalent - to reduce or eliminate their occurrence. Control checkpoints, throughout the processing steps, are defined and implemented at each production site. In the business of continuous improvement, Ÿnsect is committed to going above and beyond the simple application of these regulations, to deliver the highest product quality possible. GOING A MILE FURTHER IN TERMS OF SECURITY As strict as the regulations may be, nothing stops companies from implementing more extensive controls than what is required by law. With this in mind, Ÿnsect has gone a step further in its devotion to the safety of its products by validating various certifications such as ISO 9001 and FSSC 22000, among the most thorough, which have strict specifications and are recognized worldwide to further guarantee quality and safety within facilities. Frequent supplementary checkpoints have been added to the mandatory protocol. For example, the monitoring of the compulsory processing method needed to validate processed animal protein product commercialisation, is repeated more frequently at Ÿnsect to further ensure the security of its insect-derived proteins. Innovative analysis technologies have also been specifically developed internally for the processing of mealworm-based products. Contaminant analysis methods have been adapted to insect - and specifically mealworm - matrices. “At Ÿnsect, we fall under the same strict regime as any other operator of the animal petfood industry, but we also carry out controls that are specific to our industry, according to hazards and risks that are specific to the rearing of insects and production of Lead Cadmium Arsenic Mercury Clostridium perfingens Enterobacteriae (37°C) Salmonella All analyzed / / 20 / 10 2.0 2.0 0.1 Absence/g <10 cfu/g Absence/25 g Depending on substances <0.5 <50 <0.1 <0.5 0.07 0.144 0.06 <0.005 Absence/g <10 cfu/g Absence/25 g < LOQ (All sought active substances, among more 252 ones are below the limit of quantitation 1 Directive 2002/32/EC of 7 May 201 2 Regulation (EU) N°396/2005 3 Regulation (EU) N°142/2011 Element (µg/kg) Element (mg/kg) Element (mg/kg) Microorganism Mycotoxins Heavy Metals Pesticides Microbiology Feed material regulatory max limit1 Feed material regulatory max limit1 Feed material regulatory max limit1 2 Regulatory max limit3 YnMeal max concentration YnMeal max concentration YnMeal max concentration YnMeal max concentration Safety Hazards YnMeal vs. Regulation OchratoxinA Deoxynivalenol Aflatoxin B1 Sum of Aflatoxins B1, B2, G1 and G2

ARTICLE 24 F&A Alternative Proteins Edition April 2023 insect-based end-products,” explains Noémie Beaudoin, Quality control expert at Ÿnsect. ŸNSECT’S EXPERTISE OPTIMIZES PRODUCT SAFETY AND QUALITY The insect farming facilities are under heavy scrutiny at Ÿnsect, with rigorous quality controls. Indeed, pursuant to the European legislation, farmed insects fall under the farmed animals3 category, like any other animal. As such, they are subject to the majority of the standards that apply to livestock farming. Everything that comes in and out of the factories goes through robust internal and external controls, including raw materials and finished products. In line with the analyses carried out on the end-product, Ÿnsect strictly monitors the rearing process, thus ensuring compliance with the agri-food legislation and guaranteeing the highest of standards. Proactive on setting an example and breeding mealworms with the most relevant system possible, Ÿnsect has made innovative improvements to the breeding, monitoring and safety of insects. The safety and quality of the resulting insect-based product is therefore also enhanced. An on-site team of entomologists, agri-food and quality experts, and an external veterinarian build inhouse knowledge to guarantee insect health, biosecurity and end-product requirements. Workers receive accurate training and take part in ongoing workshops to ensure adhesion and compliance to biosecurity principles and understanding of health measures. THE NEXT GENERATION OF MEALWORM FARMING With safety in mind, the Ÿnsect facilities were built on this expertise to prevent insect and product contamination and to maximize security. Dry, compartmentalized, controlled, and stackable nurseries help maintain consistent, hygienic, disease-free, internal conditions so that production is largely unaffected by external factors. System consistency is notably increased through operational redundancy. Additionally, extra attention is paid to the diet of the mealworms. Strict restrictions4 apply as to what the mealworms can be fed, to guarantee the safety of the whole feed and food chains and avoid the presence of undesirable substances. Regular analyses of the feeding substrate are notably performed to verify microbiological quality and the absence of contaminants. This expertise has helped tailor precisely to the mealworms’ needs, improving the quality, safety and consistency of the resulting end-products. Consumption risk is similar to other farmed animal fed with plant-based substrates, but the precise control over the mealworms’ diet ensures safe food and means that the final end-product is consistent in quality over time.