SPECIAL STORY 56 FEED & ADDITIVE MAGAZINE August 2023 Krill Meal F3 Krill Replacement Product Menon Pro50FF Soy Protein Concentrate (Selecta 60) Corn Protein Concentrate (E75) Blood Meal Wheat Gluten Meal Wheat Flour Canola Oil Algae Oil Monoammonium Phospate Vitamin Mineral Premix Lysie HCI Taurine DL-Methionine Threonine Asta, pink 0 5 13 27.1 7.7 6 2.15 10.134 16.95 4.5 3.25 1.7 1.2 0.5 0.65 0.16 0.006 Ingredient (%) Experimental Treatment Prior to launching the contest, the F3 Team conducted a preliminary comparative feeding study of Atlantic salmon to evaluate whether a krill replacement challenge could be designed based on the performance of Salmo salar during 90 days of feeding. Two prerequisites were needed to hold the Challenge: (1) a plant-based feed with krill (positive control) needed to perform as well as a fishmeal control; and (2) that a plant-based feed without krill did not perform as well as the two controls above. The results confirmed that there existed a base feed that needed krill to perform as well as a fishmeal control. Using this base feed, the F3 Team could add each of the different Challenge Entrant’s Krill Replacements so that each Entrant’s feed performance could be compared against each other and against the controls. METHODOLOGY AND EXPERIMENTAL DESIGN The Challenge Host will be responsible for the proper conduct of the experiments in consultation with the F3 Team, Judges, and the Scientific Review Committee. The Trial will consist of up to twelve treatments: two controls and up to ten Entrant Experimental feeds. All treatments will be fed to Atlantic salmon in replicate tanks for 12 weeks. Throughout the trial(s) weight gain, survival and feed conversion ratio will be measured. The treatments have the following labels and composition: Control Diets: 1. Krill Negative Control (KNeg) = F3 plant-based feed* + 0% krill (5% wheat flour added) 2 Krill Positive Control (KPos) = F3 plant-based feed* + 5% krill *The F3 (fish-free feed) plant-based feed includes 6% poultry blood meal as an essential amino acid source. The F3 Team defines “plant-based” as consisting mostly of plant materials, with a small amount of (non-marine) animal protein included. Experimental Treatment diets each based on Entrant’s F3 Krill Replacement: F3 plant-based feed* + up to 5% F3 Krill Replacement from Entrants. If <5% inclusion is requested by the Entrant, the F3 Chief Scientific Officer will work with the Entrant and feed manufacturer to determine how to make up the remaining composition, likely with wheat flour. The formula for the Experimental Treatment diets is below. All treatments will undergo the same protocols, including feeding regime, water quality monitoring, growth and survival measurements. Protocols and growing conditions for the Challenge Trial will be similar to those implemented in the preliminary comparative feeding study.
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