Feed & Additive Magazine Issue 22 November 2022

November 2022 Year: 2 - Issue: 22 Joske Schuurmans, Adisseo NL B.V. Combined approach to successfully replace antibiotics Professor Jarosław Dastych, Proteon Sustainable, novel antibacterial tools for poultry Dr Stéphanie Ladirat, NUQO Securing feed intake: The last generation technologies www.feedandadditive.com Contribution of Feed Technologies to Sustainable Feed Production Gut Health Management and Performance Relationship in Poultry

Dear readers, The gut is called the second brain of the human body. Because gut health has a critical role in performing all vital activities. It significantly affects the immunity level of individuals, their general health status, and even their social lives. The stress, sadness and troubles experienced are reflected in the intestinal system. For this reason, foods that are good for the intestine are recommended when coping with distress and stress. In short, if the intestinal system collapses, the whole system collapses too… Just like the brain. Of course, this is true for animals as well as humans. Gut health is vitally important for all living things, including our farm animals. The general health and immunity of the animal are significantly affected by guts. In addition, it should not be ignored that there is a strong link between performance and quality in animal production and gut health. As in humans, the most effective way to maintain gut health in animals is primarily nutrition. Feeding or correct nutrition in livestock is a multifaceted concept which varies according to the type of animal, age and environmental conditions. But one of the main criteria is the digestibility and absorption of nutrients. In this month's issue of Feed & Additive Magazine, we tried to cover this vital issue on poultry basis. Poultry is the segment of livestock where intestinal problems are most common and most affected. In the past, antibiotics were widely used as growth promoters, especially in the poultry sector. This gave manufacturers an advantage in terms of performance. However, due to increasing antibiotic resistance, the use of AGPs is banned or restricted in many regions, especially in Europe. Meanwhile, worldwide demand for poultry products continues to increase rapidly. Therefore, poultry farmers need to focus on more things to produce without AGP, to respond to increasing demand, and to increase the profitability and efficiency of their businesses. Gut health is one of them and coming first. We hope this issue will make a good contribution to poultry farmers in terms of gut health, AGP-free production, high performance and profitability in poultry. Enjoy your reading… Publisher Muhammed Akatay [email protected] Responsible Manager Özlem Buyruk Akatay [email protected] Editor-in-Chief Derya Yıldız [email protected] Editors James Watson Bahadır Büyükkılıç [email protected] Design Alper Kandemir [email protected] Web Design Caner Cem Pulat [email protected] Proofreading Lisa Atakul [email protected] Circulation and Data Responsible Amine Şengün [email protected] Ataturk St. Metropol Istanbul Ste. Block C1, No:376 Atasehir, Istanbul/TURKEY Phone: +90 850 220 5064 E-Mail: [email protected] Web: www.feedandadditive.com Publication Type: Periodic (Monthly) EDITOR Second brain: Guts… Derya YILDIZ © Three Plus Media | All rights reserved. Reproduction, in whole or in part, is prohibited without the written permission of the publisher. *DISCLAIMER: All views and ideas expressed in articles published in the magazine reflect the point of views of the author(s), not that of the Editor-in-Chief, Editors, Editorial Board or Publisher of Feed & Additive Magazine. The Editor-in-Chief, Editors, Editorial Board and Publisher assume no responsibility or liability in such cases. The publisher does not give any warranty and representation to the accuracy or completeness of the contents and data published in the magazine. All responsibility of the contents, articles and information published in the magazine belongs to the authors. The publisher and the authors cannot be held responsible for any damages resulting from the use of the whole or part of the content of the magazine. feedandadd i t i ve


C O N T E N T S 74 CHOLINE SUPPLEMENTATION TO TRANSITION COWS: A HEALTHIER LAUNCH OF HER LACTATION Marcos Zenobi, Balchem 78 SECURING FEED INTAKE: THE LAST GENERATION TECHNOLOGIES Dr Stéphanie Ladirat, NUQO ARTICLES Nutreco and SHV promote sustainable aquaculture in Africa 8 Layn Natural Ingredients launches natural feed additive TruGro® MYC 12 Icy Waters and CAT partner to accelerate genetic improvement in Arctic Charr 94 New study: Krill improves survival of seabream larvae 88 MicroBioGen opens state-of-the-art labs in Sydney 96 New joint venture opens fishmeal facility in Oregon 90 Mt Cook Alpine Salmon to build innovative land-based salmon farm 93 NEWS INTERVIEW SUSTAINABLE, NOVEL ANTIBACTERIAL TOOLS FOR POULTRY 64 Professor Jarosław Dastych Proteon Pharmaceuticals REPORTS GLOBAL FEED ENZYMES MARKET 82 68 WHO HAS A STAFF SHORTAGE? Wayne Cooper, Anderson Feed Technology 70 IDEAL EXTRUDER TEMPERATURE TO PRODUCE BEST FULL FAT SOYBEAN MEAL Danny Patino, Trouw Nutrition & Michael Joseph, North Carolina State University SPECIAL STORY

NEWS 6 FEED & ADDITIVE MAGAZINE November 2022 Officially opened in Guayaquil, Ecuador, Skretting Aquaculture lnnovation (AI) Guayas Research Station is the latest investment made by the world’s leading aquaculture feed company aimed at fast-tracking the shrimp farming industry’s introduction of more sustainable and innovative feeds. The new EUR 6.1 million facility – a world-class R&D station that is fully dedicated to shrimp farming – establishes the ideal platform for Skretting to further expand its knowledge of shrimp nutrition and health, and to apply this intelligence on-theground in one of the global seafood economy’s most important shrimp producing regions. Incorporating the latest technologies, the station comprises fully-equipped laboratories and state-ofthe-art experimental units that will perform critical trials on whiteleg shrimp (Litopenaeus vannamei). Additionally, it houses a battery of tanks that will Skretting Aquaculture lnnovation Guayas Research Station opens in Ecuador Skretting announced it has opened the Guayas Research Station, the company's latest investment in the shrimp farming industry in Guayaquil, Ecuador. Unibio, one of the leading sustainable protein companies, has announced that its strategic licensing partner, the Doha-based industrial biotech investor Gulf Biotech, has reached the final stage before the construction of the first single-cell protein (SCP) plant in Qatar by entering the Front-end Engineering Design Stage (FEED). Qatar is a country that is rich in natural gas resources but has little or no farmland or the ability to produce protein for its population or the surrounding region. By using Unibio's technology to produce protein locally, it helps to utilize their abundant resources, to directly enhance food security. The plant will use Unibio’s U-Loop® technology, where methane is converted through continuous fermentation to produce Uniprotein®. The Uniprotein® produced in the plant will be used as a protein supplement in feed for fish and animals to supplement the deficit due to shortages of fish meal or soy. The production of Uniprotein® is highly resource-efficient and sustainable compared with the production of traditional protein. Relative to soy production, Uniprotein® uses no arable land and significantly less water. Initial production is estimated to be 9,000 tonnes of protein per year. According to Unibio's statement, Gulf Biotech, represented by His Excellency Hitmi Al-Hitmi, has shown its commitment to using innovative technology and Qatar’s natural resources to tackle the highly pressing challenge of producing sustainable food for the world’s rapidly growing population. Gulf Biotech has completed their feasibility study on the viability of the plant. The FEED for the development of the plant is due to be completed by early 2023. “We are absolutely delighted with the progress made together with Gulf Biotech in reaching the FEED stage at what will be Qatar’s first single cell protein plant. We need innovators and visionaries if we are to provide food security for the world’s growing population in a sustainable way, especially at a time when the global cost of food continues to rise. Qatar’s abundance of methane makes it an ideal region to use our technology," said David Henstrom, CEO of Unibio. Unibio and Gulf Biotech to deliver Qatar's first Uniprotein® plant

NEWS FEED & ADDITIVE MAGAZINE November 2022 7 Calysseo, a joint venture between worldwide animal nutrition leader Adisseo and protein innovator Calysta, has switched on its first industrial-scale alternative protein facility to produce FeedKind® in China. The world’s first industrial-scale facility to produce FeedKind®, a protein ingredient that needs no animal or plant matter to produce, has switched on. Calysseo, a joint venture between Adisseo and Calysta, will initially produce 20,000 tonnes of protein per year from the facility in Chongqing, China. With startup operations complete, the fermenter will become world’s largest single protein production facility. First deliveries, of FeedKind Aqua®, will be made to customers soon, making it the first alternative fermented protein to address the sector at scale. “Food security has increasingly come under the spotlight in recent months, but we are now entering a new era of sustainable food production. Calysseo brings to the market a new way of making high-quality protein – and does so using a naturally-occurring microbe that makes the resulting protein non-GMO, something that is increasingly demanded through the supply chain," said Jean-Marc Dublanc, CEO of Adisseo. “We have a long-running commitment to improving the security and sustainability of the feed ingredient market and Calysseo fulfils a significant part of that pledge. We will provide Chinese customers with a reliable, domestically available supply of protein that meets their specific needs; produced in China for China. We are looking forward to working with our customers as they begin to integrate this product into their supply chains.” The Chongqing facility will produce FeedKind Aqua® for use in fish farming, allowing farmers to replace fishmeal and soy with a high-quality ingredient that needs no arable land and no plant matter. Initially, production will be available for use in China, the world’s largest aquaculture market. World’s first industrial-scale alternative protein facility switches on allow multiple studies to be conducted in conditions that mimic commercial production environments. Skretting AI Guayas Research Station will study all stages of shrimp production – from larvae through to harvest size animals. Simulations and evaluations will include health and welfare parameters, as well as growth and feed efficiency. Another key focus area is the digestibility of new and existing feed ingredients and balanced diets. For this purpose, sophisticated biotechnologies will be implemented and further developed. The new station’s team will have the on-hand support of Skretting’s global network of scientific experts and its overarching global research units. Together, they will help Skretting Al Guayas Research Station better understand how shrimp can be helped to grow faster and larger and be more resilient to disease and environmental challenges so that innovations can be developed that turn these crucial findings into practical industry solutions.

NEWS 8 FEED & ADDITIVE MAGAZINE November 2022 Aquarech wins 2022 Nutreco Feed & Food Tech Challenge Nutreco and SHV promote sustainable aquaculture in Africa Nutreco has announced that Aquarech is the winner of the 2022 Nutreco Feed & Food Tech Challenge (NFFTC). Aquarech won first place for its work to build Kenya's first fish farming supply chain platform. Aquarech will receive a validation trial supported by Nutreco experts to further develop its technology. Jury member Alex Obach said, “Congratulations to Aquarech for winning the 2022 NFFTC! Through their Aquarech platform, fish farmers in Kenya and Africa can buy top-quality feed, sell fish, and learn about best fish farming practices to improve their incomes and in general to improve the entire supply chain of high quality fish to feed to local population. We look forward to supporting Aquarech in further developing their innovative concept.” This year’s NFFTC received over 100 submissions from 48 countries. These were narrowed down to 15 finalists, who received support from Nutreco mentors to develop a pitch deck and business case that they defended at a virtual pitching event in June. A jury of industry experts selected five top candidates from these finalists. The top five attended an in-person finale this week in the Netherlands to pitch their ideas one last time and network with other start-ups, investors, and Nutreco experts in a program that included speakers and interactive discussions. “This year’s entries showed a great deal of promise in helping our industry solve the challenges we face as we work to produce more protein more sustainably and achieve our purpose of Feeding the Future. We’d like to thank everyone who participated in this year’s Challenge!” said Nutreco’s Director of Venturing & Business Development Joost Matthijssen. Nutreco and its parent company SHV have partnered with FoodTechAfrica and African Parks on an initiative to promote sustainable aquaculture in Africa. In addition to financial investments, Nutreco and SHV will contribute their deep knowledge and experience in creating sustainable ways to feed the future, while also driving forward conservation-led economies. The first project under the initiative, the Gishanda Fish Farm in Rwanda, opened and will help meet Rwanda’s growing demand for protein. “We are proud to announce the partnership with SHV, FoodTechAfrica and African Parks. It is estimated that more than half of the projected global population growth in coming decades will take place in Africa. Our purpose of Feeding the Future is focused on helping produce more sustainable protein to feed the growing population here and across the globe. As food insecurity is a global issue, we believe in the great potential of addressing it through partnerships such as this one. We’re grateful to SHV for supporting this project and believe that being part of the SHV group greatly enhances our ability to make a difference in pursuit of Nutreco’s purpose,” said Nutreco CEO Fulco van Lede. The Gishanda Fish Farm is the first project underNutreco and SHV announced that they have joined partnership with FoodTechAfrica and African Parks to promote sustainable aquaculture in Africa.

NEWS FEED & ADDITIVE MAGAZINE November 2022 9 way as part of the initiative to provide sustainable aquaculture in Africa. The fishery officially opened on Tuesday October 18 outside African Parks-managed Akagera National Park in Rwanda and will continue to be developed over the coming years. The project is using innovative solutions to accelerate aquaculture development in Rwanda, provide employment opportunities to surrounding communities and deliver more sustainable protein. The Gishanda project is focused on the construction of a Recirculating Aquaculture System (RAS) tilapia nucleus demo farm. Skretting will share knowledge on sustainable agriculture with local farmers. The farm will produce up to 30 tonnes of tilapia annually, of which at least 10% will be supplied locally at affordable prices. It will also produce up to 1.5 million tilapia fingerlings on an annual basis, of which 110,000 will be retained for farm production, one million sold commercially to bolster the Rwandan aquaculture sector and 300,000400,000 used to sustainably restock the Akagera National Park lakes. In addition, Gishanda will serve as a model of circular agriculture with the farm effluent fertilizing an on-site organic vegetable farm. The sustainable aquaculture initiative started as a cooperative effort between FoodTechAfrica, a public-private partnership headed by Larive International focused on improving food security in East Africa, of which Nutreco’s Skretting aquaculture business line is a partner, and African Parks, a leading conservation organisation that manages a diverse portfolio of 22 protected areas. It will focus on helping solve challenges within the aquaculture supply chain in Africa by promoting protein production in lakes outside of the parks. Your customized feed additive solutions to protect poultry health Reaching optimal poultry performance requires considering all aspects of the production chain. Selko brings together products, services and knowledge in an integrated approach. We support feed, farm and health management to achieve customer’s long-term objectives. THE BENEFITS Maintain gut health Increase performance Decrease the need for antibiotics www.selko.com

NEWS 10 FEED & ADDITIVE MAGAZINE November 2022 Scoular, a multi-generational agribusiness serving farmers since 1892, has reached an agreement to acquire Walker Products’ grain handling facility in Lincoln, Kansas. Scoular and Walker expect the acquisition to close in the coming weeks. The facility offers 1 million bushels of storage for wheat, milo, soybeans and corn, with access to rail. The Walker family has owned the central Kansas country elevator since 1954. “Craig Walker and his wife Donna are great allies to area farmers, as well as the Lincoln community,” said Scoular Regional Manager Brian Ring. “Scoular’s goal is to uphold the high standard of integrity and compassion set by the Walker family in this community.” Scoular, headquartered in Omaha, Nebraska, operates a Midwestern grain handling network that includes more than 50 facilities in Missouri, Iowa, Kansas, Colorado and Nebraska. The Lincoln operation will become part of Scoular’s Solomon Valley group, which includes facilities within a 50-mile radius in Salina, Minneapolis, Ada and Downs, Kansas. Scoular to acquire Walker Products’ facility in Kansas Cargill strengthens presence in Ecuadorian shrimp feed market After partnering with Naturisa S.A. in 2015 to bring world-class nutritional formulation expertise to Ecuador, Cargill is now partnering with Skyvest EC Holdings S.A. to meet the growing demand for premium feed for shrimp farming. Cargill, together with its partner Naturisa S.A., has reached an agreement to partner with Skyvest EC Holding S.A. to create a new joint venture that will serve Ecuadorian shrimp farmers’ increasing demand for high-quality shrimp feed. Cargill first brought its worldclass nutrition formulation expertise to Ecuador in 2015 when it partnered with shrimp-farming company Naturisa to build and operate a shrimp-feed manufacturing facility under the name Aquacargill del Ecuador. “Since that time, the Ecuadorian shrimp farming industry has continued to grow, and there is a need to meet the increased demand for high-quality feed,” said Helene Ziv-Douki, president of Cargill’s aqua nutrition business. Through this new joint venture with Skyvest, Cargill will expand its shrimp-feed production capabilities to own and operate the shrimp-feed production facility currently owned by Skyvest subsidiary Empagran—located in Guayaquil, Ecuador. The feed plant that will be operated by Cargill was built in 2018, has a production capacity of 156,000 metric tons and employs more than 200 people. Skyvest’s shrimp-feed production facility in Guayaquil, Ecuador | Photos courtesy of Empagran

NEWS FEED & ADDITIVE MAGAZINE November 2022 11 New paper: Live yeast improves pigs’ resistance to heat stress According to a paper published in the British Journal of Nutrition, the live yeast Saccharomyces cerevisiae boulardii CNCM I-1079 improves pigs’ resistance to heat stress through improved feeding behavior and metabolism. A recently published paper in the British Journal of Nutrition gives further insight into the benefits of the live yeast Saccharomyces cerevisiae boulardii CNCM I-1079 (LEVUCELL SB) on pig performance during heat stress. The study was conducted in partnership with the INRAE team of Etienne Labussière, David Renaudeau, and doctoral student Aira Maye Serviento. The paper detailed their trial with fattening pigs in simulated heat stress. “Aira Maye Serviento collaborated with us for the past three years conducting her Ph.D., and I’d like to congratulate her for her hard work and thesis. Her work brings a significant contribution to our understanding of the effect of heat stress on pigs and deeper insights on the mechanisms of the probiotic yeast to mitigate it,” comments Mathieu Castex, R&D Director for Lallemand Animal Nutrition. "The benefit of LEVUCELL SB to the ability of growing pigs to cope with heat stress has already been documented, and more recent studies indicated a positive effect at the microbiota level. The present study aimed at investigating whether the live yeast had an effect on feeding behavior and energy metabolism," Lallemand Animal Nutrition said in a statement. Si-Ware partners with AB Vista to offer NeoSpectra platform to animal feed industry NeoSpectra by Si-Ware, the creator of NeoSpectra material analysis solutions FT-NIR spectrometers, announced a partnership with AB Vista, a global leader in feed additive products and technical services to animal feed and farming sectors, to offer their customers a complete portable NIR solution based on the NeoSpectra platform. The complete solution will include industry-leading NeoSpectra Scanners, world-class mobile apps, secure cloud portal for data reporting and evaluation, and AB Vista INGOT calibrations for feed ingredients, compound feeds and forages. The NeoSpectra platform has created the first and largest LabStore in the market, offering thousands of calibration models to users from trusted partners around the globe such as labs, researchers, and universities. In April 2022, Aunir, the NIR calibration division of AB Vista and the world’s largest independent calibration provider, partnered with NeoSpectra to offer INGOT calibrations as one of the trusted partners on the NeoSpectra LabStore. The AB Vista INGOT calibrations through the NeoSpectra platform will soon be available to AB Vista customers in the USA and Canada direct from their local AB Vista technical service representative.

NEWS 12 FEED & ADDITIVE MAGAZINE November 2022 Layn Natural Ingredients, one of the world’s largest vertically-integrated manufacturers and innovators of natural, functional botanical extract ingredients and solutions has launched TruGro® MYC, a potent, polyphenol-rich botanical extract solution for use as an animal feed additive to reduce the negative effects of mycotoxins on production animals. According to the company statement; mycotoxins in animal feed have been shown to increase oxidative stress in production animals, which can have numerous detrimental effects in production. TruGro MYC contains potent polyphenols – proanthocyanidins and catechins – which are powerful metabolic antioxidants that have been shown to reduce the negative pathenogenic effects mycotoxins have on production animals. The reduction of oxidative stress can decrease the inflammatory response, increase enzyme synthesis, contribute to homeostasis maintenance and support immune health. Layn Natural Ingredients launches natural feed additive TruGro® MYC AstaReal has announced a new strategic partnership with IQI Petfood Ingredients to bring its innovative, science-based, health ingredient to the pet food market in Europe. The partnership combines the strengths of both companies to form a greater whole. AstaReal will provide its well-researched, natural algae-derived ingredient, while IQI Petfood will act as a growth engine for Europe, connecting AstaReal with local and regional players in the pet food industry looking for high-quality, pure solutions. AstaReal partners with IQI for European pet food market IQI Sales Director Europe Roeland Vreeswijk, AstaReal Key Accountmanager Astrid van der Staaij & IQI Innovation Manager Geert van der Velden. AstaReal, natural astaxanthin producer, has announced that it has partnered IQI Pet Food to bring its ingredients to the pet food market in Europe. Layn Natural Ingredients announced that it has launched TruGro® MYC, natural animal feed additive designed to help reduce the pathogenic effects of mycotoxins.

NEWS FEED & ADDITIVE MAGAZINE November 2022 13 Researchers at Central Queensland University (CQU) will soon commence an experiment to test the ability of Australian climate technology company Rumin8’s additive to reduce methane emissions from cattle when delivered in their drinking water. The experiment is testing a range of methane-reducing compounds to determine if they can be safely and effectively delivered to cattle via automated water medication systems to decrease enteric methane emissions. Laboratory trials of Rumin8’s additive – which reproduces nature’s ingredients to develop climate friendly feed supplements that reduce methane emissions from livestock – indicate it is effective when applied in either a powder, oil or liquid form. The experiment is part of a project funded by Meat and Livestock Australia and Advance Queensland and is being implemented by CQU and DIT AgTech. “The CQU trial will be our first animal trial using a water-based application and our lab results give us confidence that it will perform in the field,” said Rumin8 Managing Director David Messina. “Most of the current methane reducing additives use feed-based delivery to livestock. Successful delivery of the methane reducing additive through a water trough, if successful, could provide an opportunity for rangeland and pasture-based beef production systems to reduce their methane emissions, opening up a significant new market for Rumin8,” he added. “At any one time there are about 24 million cattle in Australia eating grass and it’s important that we provide a methane solution to those farmers who are breeding and finishing rangeland and pastured-fed cattle.” New cattle trial to test water-based delivery of Rumin8’s methane reducing additive Supplant D Creating chain value • Supports food safety • Improves protein efficiency • Better livability & animal welfare • Enhances processing yields agrimprove.com Aromabiotic® Poultry

NEWS 14 FEED & ADDITIVE MAGAZINE November 2022 United Petfood acquires Gold Line Feeds International pet food producer United Petfood announced the acquisition of Gold Line Feeds, United Kingdom based animal feed manufacturer. United Petfood, announced the takeover of Gold Line Feeds in the UK. The acquisition strengthens the position of United Petfood as full-service pet food producer. United Petfood Group will take over the brands and customer base of the factory. They will house production and distribution at their current sites and move administrative functions to United Petfood UK. Gold Line Feeds is an independent animal feed manufacturer with 80 years of experience situated in Northamptonshire, UK. The British factory supplies about 20.000 tonnes of dry pet food each year. This takeover strengthens United Petfood’s position within the pet food manufacturing market and in particular within the UK. United Petfood Group operates out of 20 pet food facilities situated in 8 different European countries. The takeover is in line with the strategy of United Petfood to reinforce and grow the business. Zinpro® launches breakthrough innovation in dairy nutrition Zinpro Corporation, the world’s leading innovator and manufacturer of highperformance organic trace minerals and animal nutrition solutions, has launched its newest breakthrough, Zinpro® IsoFerm®. Zinpro Corporation has announced that it launched Zinpro® IsoFerm®, a breakthrough innovation and essential nutrient which increases feed efficiency, improves sustainability and drives profitability in dairy cattle nutrition. “Partnership is key to driving dairy profitability and sustainability,” says Rob Sheffer, Zinpro president and CEO. “Zinpro® IsoFerm® is a breakthrough. We are empowering dairy nutritionists to elevate how they formulate diets, unlocking new levels of feed efficiency and operational sustainability. Dairy farms face volatile market pressures and feed costs. It’s critical that we come together to improve dairy cow performance and the bottom line.” A one-of-a-kind breakthrough innovation in dairy ration formulation, Zinpro® IsoFerm® is an essential nutrient that fuels rumen function by directly feeding the fiber-digesting microbes. By enhancing fiber digestibility, cows can thrive with fewer inputs, improving milk production and operational efficiency often with less dry matter intake. “At Zinpro, we are constantly working to provide new and better solutions to transform animal productivity and strengthen our partnerships,” said Sheffer. “We are excited to help dairy nutritionists and producers achieve peak operational efficiency and performance. Together we’ll embrace the next frontier of dairy nutrition.” The company explained that Zinpro® IsoFerm® is currently available throughout the U.S. and will be expanding to select countries in 2023.

NEWS FEED & ADDITIVE MAGAZINE November 2022 15 Bioiberica strengthens its presence in Japan animal nutrition market Bioiberica, a global Life Science company committed to improving the health and well-being of people, animals and plants, has partnered with Aska Animal Health Co. and Shintoa Corporation to distribute its Nucleoforce™ ranges, and eventually its Palbio™ portfolio, in Japan. As leading local suppliers of feed ingredients for animal nutrition, both companies will support Bioiberica in reaching and engaging new customers across Japan to deliver cutting-edge developments in the animal health space. The deal with the two local partners enables Bioiberica to compete in a complex and demanding market, while also extending its presence in the Asia Pacific region. Aska Animal Health Co. – Bioiberica’s official distributor in Japan – will initially import the company’s Nucleoforce Aqua™ and Nucleoforce Livestock™ ingredients, which offer a balanced concentrate of free nucleotides and active precursors obtained from yeast. As part of the agreement, Aska will help to manage important regulatory procedures and local R&D trials. Meanwhile, the Shintoa Corporation will oversee the promotion and sale of the ingredients. Longer-term, the trio has plans to bring Bioiberica’s hydrolysed protein range, Palbio™, to the Japanese market too – it is currently pending registration before distribution begins. BrightPet acquires Bravo Pet Foods BrightPet Nutrition Group, a leading marketer and manufacturer of premium and super-premium pet foods, treats and care products, has announced that it has completed the strategic acquisition of Bravo Pet Foods, a pioneer in frozen raw and freeze-dried pet diets. The transaction by BrightPet, a portfolio company of A&M Capital Partners (AMCP), a middle-market private equity investment fund, is the second in two years, reflecting the company’s commitment to a M&A strategy that bolsters its branded portfolio while extending capabilities in high growth market segments. “The Bravo brand is widely known and has been respected for more than 20 years. We believe Bravo’s commitment to innovation, premium ingredients and the highest standard of manufacturing aligns well with BrightPet,” said Dave Kowal, CEO of BrightPet. “This acquisition supports our vision to be the most valued pet platform in the industry. By expanding our branded portfolio and strengthening our production capabilities, we expect to continue the growth trajectory BrightPet has established over the past few years.” Melinda Miller, former CEO of Bravo, will join BrightPet as the Vice President of Bravo Brands. Melinda is a leading expert in nutrition, formulation and innovation, and she will help lead the integration of capabilities through the BrightPet portfolio. “We could not be more excited for Bravo to join the BrightPet family of brands,” said Miller.

NEWS 16 FEED & ADDITIVE MAGAZINE November 2022 According to a new report by Rabobank, the value of the global seafood trade grew by $13 billion, reaching a new peak of more than $164 billion in 2021. The value of the global seafood trade reached new heights in 2021. A new Rabobank report expects this strong rebound to endure through 2022, as the world continues to emerge from the Covid-19 pandemic. Major markets like the US and Europe have fully recovered, while China is gradually returning to pre-pandemic import levels. Premium aquaculture has been the decade's winner – in particular, farmed salmon and shrimp. Fish is one of the world's most traded food commodities, with demand expected to increase another 15% in the next decade. The most traded animal protein, seafood has a trade value 3.6 times the size of beef, five times that of pork, and eight times the size of the global poultry trade. In 2021, the value of the global seafood trade grew by $13 billion, reaching a new peak of more than $164 billion. The US is the fastest-growing market for seafood imports, with demand driven by health- and sustainability-conscious consumers, particularly among millennials and baby boomers. China is one of the most important seafood markets, with a growing middle class interested in premium products. Wild catch and aquaculture production do not satisfy local demand, leaving China increasingly reliant on imports, especially for premium species like shrimp, crab, and salmon. Farmed salmon was one of the winning proteins of the decade, supported by growing demand for healthy and convenient protein, especially in Europe and the US. Shrimp is the most traded seafood species, with short production cycles that allow exporters to respond quickly to demand changes. According to Rabobank, the shrimp trade is at an inflection point. Demand and prices have come down while costs (feed, freight, energy) are still high, impacting farmer profitability. RaboResearch: Global seafood trade sees record growth, with premium aquaculture in the lead Zoetis Inc. announced it has completed the acquisition of Jurox, a privately held animal health company that develops, manufactures and markets a wide range of veterinary medicines for treating livestock and companion animals. Jurox’s operations are based in Australia, with additional regional offices in New Zealand, the U.S., Canada and the UK. Financial terms of the transaction, which was announced in August 2021, were not disclosed. The acquisition of Jurox brings Zoetis a range of important products primed for greater global expansion; a valuable animal health portfolio, including Alfaxan®, a leading anaesthetic product for companion animals; and high-quality local R&D and manufacturing operations in Australia, Zoetis’ fourth largest market based on revenue in 2021. Jurox develops and manufactures more than 150 products in areas such as parasiticides, anti-infectives, anaesthesia, cardiology and reproduction for animals. “We are grateful to the O’Brien family for entrusting the future of this family-owned business to Zoetis,” said Zoetis CEO Kristin Peck. “Jurox’s portfolio plays to the strengths of our core business and will be a complementary fit with the solutions we deliver to veterinary professionals, livestock producers and pet owners around the world,” added Peck. Zoetis completes acquisition of Jurox



ISSUE FOCUS FEED & ADDITIVE MAGAZINE November 2022 19 The determination of a nutritional matrix by modelling Caroline Donaldson & Sandra Chamusco & Manu De Laet, Delacon ROI: What can phytogenic feed additives deliver for broiler producers? Dr. Ruturaj Patil, EW Nutrition Can gut health save the pocket of a farmer as well as … The planet? Paulina Abramowicz-Pindor, AdiFeed Co Ltd. How fatty acid esters contribute to gut health in animal production? Dr. Clarisse Techer & Pauline Duménil, Mixscience, Avril Group Seaweed can positively influence the poultry microbiome Hadden Graham, Ocean Harvest Technology Can mitigating feed-source pathogens protect gut health and poultry performance? Matt Oldnall, Anitox Precise microbiome modulation: A new era in microbiome research in poultry production Cristiano Bortoluzzi & Jack Geremia, DSM Animal Nutrition Benefits of Bacillus Coagulans DSM 32016 supplementation on performance in broilers Dr. Lydia Zeibich, Biochem – Feed Safety for Food Safety Combined approach to successfully replace antibiotics Joske Schuurmans, Adisseo NL B.V. Microflora modulation by monovalent copper & fat digestibility, a relation? Alessandra Monteiro & Axel Minetto, Animine

ISSUE FOCUS 20 FEED & ADDITIVE MAGAZINE November 2022 In the search for alternatives to the intensive use of antibiotics in poultry farming, glycerides seem very promising. In a recent trial a combination of glyceride-based additives for feed and drinking water was successful in replacing the preventive use of antibiotics in broilers. Broilers were able to maintain growth performance and health status and even showed an improved immune response. For years feed antibiotics were the number one in combating infections and intestinal disorders that are inevitable in the industrial poultry industry. They significantly improved weight gain, feed conversion and increased safety of the poultry population. However, concerns rise about pathogens becoming resistant to different types of antibiotics and antibacterial therapeutic agents. Therefore, it is of urgent matter to look for new supplements that help optimize animal health and performance and improve the safety and productivity of poultry. KEY ROLE FOR GLYCERIDES In their attempts to produce broiler diets without preventive antibiotics feed manufacturers have been applying conventional organic acids, and later also medium-chain fatty acids, for a long time. Which is not surprising as the mode of action of these acids has long been known and their effectiveness to support growth performance and gut health in broilers is extensively demonstrated in practice. However, new information reveals that the same organic and medium-chain fatty acids can be used much more efficiently. Even more, in the form of 'glycerides' they could play a key role when it comes to replacing preventive antibiotics in poultry farming. How? Let’s take a closer look at these glycerides. WHAT ARE GLYCERIDES? Glycerides are special molecules produced by Adisseo NL (formerly FRAmelco) in which a short or medium chain fatty acid is linked to a glycerol backbone, see Figure 1. The molecule that is created this way has special properties that are very useful to the bird. In contrast to conventional short chain and medium chain fatty acids, glycerides are pH independent and less susceptible to enzymatic breakdown, allowing them to COMBINED APPROACH TO SUCCESSFULLY REPLACE ANTIBIOTICS Joske Schuurmans Global Marketing Manager Adisseo NL B.V. Figure 1. Creating glycerides, like alphamonoglyceride, 1,3-diglyceride and triglyceride.

ISSUE FOCUS FEED & ADDITIVE MAGAZINE November 2022 21 be active throughout the entire gastrointestinal tract. These unique properties make glyceride-based products highly efficient. Scientific research has proven that glycerides indeed have a much stronger antimicrobial effect compared to their ‘free’ counterparts. Moreover, specific glycerides like those of lauric acid, are known for their potential anti-viral, immune-modulating and anti-inflammatory effects. And the good news is: the occurrence of resistance against these glycerides is very unlikely as supported by science. COMBINED APPROACH Depending on the chain length of the fatty acid different glycerides can be obtained, each with their own affinity for certain pathogens. Glycerides of short chain fatty acids such as propionic (C3) and butyric acid (C4) are mainly active against Gram-negative bacteria. In contrast, glycerides of medium chain fatty acids like capric (C10) and lauric acid (C12) are more efficient against Gram-positive bacteria. They are mostly used in blends because of the intended synergistic effect, as described by literature. This combined approach of non-antibiotic supplements could be strong enough to effectively replace the preventive usage of antibiotics. PROVEN IN PRACTICE To test this combined approach a trial was conductA special role is reserved for glycerides of lauric acid (C12), available as the feed additive FRA® C12 Dry. Find out the three reasons why the glycerides of this specific medium chain fatty acid support broilers’ natural immunity. 1. Antipathogenic effect as first line of defense Next to their proven antibacterial properties, lauric acid glycerides possess antiviral properties against fat enveloped viruses as shown by in vitro studies. They are found to affect the viral fat-envelope, causing leakage and at higher concentrations a complete breakdown of viral particles. 2. Reduction of the inflammatory response Lauric acid glycerides also reduce the expression of pro-inflammatory cytokines thereby avoiding overreactions of the immune system when small challenges occur. In this way broilers are able to save more energy for growth. However, in case of viral challenges, an increase of for instance interferon-gamma (IFN-γ) has been observed. This is important as IFN-γ is involved in inhibition of viruses. 3. Boost vaccination strategy Moreover, according to a new study published in Journal of Applied Poultry Research, glycerides of lauric acid also stimulates the immune response of broilers after vaccination against Infectious Bronchitis, one of the most important diseases in the poultry industry today. Significantly higher amounts of antibodies were found in birds receiving FRA® C12 Dry compared to the vaccinated control birds, see Figure 2. Together with the observation that more birds were able to establish faster clearance of vaccine-derived IBV, this may suggest that lauric acid glycerides strengthen the immune response. The authors point out that the higher number of antibodies found in the animals receiving FRA® C12 Dry in their feed may result in better protection against future infections. C12 in the spotlights Figure 2. FRA® C12 Dry improves the immune response in Infectious Bronchitis vaccinated broilers (De Gussem et al., 2021).

ISSUE FOCUS 22 FEED & ADDITIVE MAGAZINE November 2022 ed at a commercial broiler farm (Ross 308). Broilers in the control group got the full spectrum of antibiotics: at the hatchery they were injected with ceftiofur, via the drinking water they received enrofloxacin, colistin, trimethoprim (day 1 to 5) and ciprofloxacin (day 18 to 21) and via the feed they were given spectinomycin, lincomycin (day 0 to 9) and virginiamycin (whole period). To replace this intensive use of antibiotics, a specific program has been designed with a combination of three glyceride-based products: FRA® C12 Dry and FRA® Butyrin Ultra Dry in the diet and FRA® AC34 Liquid via drinking water. Comparable growth performance Results show that this strong combination was able to compete with the intensive use of antibiotics: broilers without any antibiotic performed evenly well as the control group. No differences were found between the control and treatment group regarding survival rate, feed conversion ratio and EPEF. They even showed an improved daily weight gain of 1.3%. Comparable health status Also antibody titer values against Newcastle Disease (Figure 3), Infectious Bronchitis Virus and Infectious Bursal Disease Virus (also known as Gumboro disease) were higher for the treatment group, suggesting an immunostimulating effect of glycerides. Essential blood parameters, intestinal morphology and microbiota as well as development of intestinal lymphoid tissue, cecal tonsils and pancreas were comparable between both groups, indicating that broilers receiving a combination of FRA® products without antibiotics were well developed and able to maintain a good health status. CONCLUSIONS Compared to conventional organic acids and medium chain fatty acids, their glycerides are much more effective in supporting broiler performance, health status and natural immunity. This is mainly due to their unique molecular structure and is probably the reason that they are gaining popularity in broiler farming. The results from this study indicate that a strong combination of FRA® glyceride products is able to replace the preventive use of antibiotics provided via the feed and drinking water without compromising animal health and performance. Broilers even showed an improved immune response as shown by the higher number of antibodies against typical poultry diseases. This possibly provides better protection in case a viral challenge occurs. Data in this article is based on our current knowledge and experience. Since many factors can affect the performance of our products during and after application, processors are responsible for conducting their own tests and investigations. Certain statements may not apply in all geographic regions. For specific advice please contact Adisseo NL. Figure 3. Blood serum antibody titer value against Newcastle Disease (ND) at day 22 and day 40 of life for the control group (preventive antibiotics; AGPs) and treatment group (FRA® glyceride combination).

ISSUE FOCUS FEED & ADDITIVE MAGAZINE November 2022 23 ANTIBIOTIC REDUCTION C12 Support natural body defences with FRA® C12 Immune response Growth performance Decreased FCR Read why glycerides in FRA® C12 are proven to be successful in replacing the preventive use of antibiotics in broilers. www.adisseo.com

ISSUE FOCUS 24 FEED & ADDITIVE MAGAZINE November 2022 In animal feed, fat and oil are important sources of energy. They have to be absorbed and metabolized to be efficiently used by the host, driving the energy to body weight gain. As fat sources represent an important cost in formulation, some feed additives were developed to enhance fat digestion and improves performance. Monovalent copper is one of them with a specific mode of action now well described. 1. ABSORPTION OF LIPIDS IN MONOGASTRIC The first and limiting step of lipid absorption is the emulsification process, which happens thanks to the bile salts action. They coat the fatty acid, decreasing the size of fat globule and providing more surface area for lipase action. Thanks to their action, the lipase can transform the fat globule into micelles which can be easily absorbed in the intestine. Bile acids (BA) are synthesized from cholesterol in the liver (primary BA), conjugated to glycine or taurine (conjugated BA) and stored in the gallbladder to be released in the intestine. In their conjugated form, they are efficient emulsifiers improving lipid digestion. However, they can be deconjugated by the gut microbiota via the enzyme bile salt hydrolase (BSH), and also be converted into secondary BA by the 7α-dehydroxylation. These enzymatic reactions done by the microbiota are not profitable for the animal metabolism: deconjugated BA are not efficient to emulsify fat; secondary BA can be toxic to the body if in high concentrations. The ideMICROFLORA MODULATION BY MONOVALENT COPPER & FAT DIGESTIBILITY, A RELATION? “High dietary levels of copper (Cu) are being used since long time to promote the growth of monogastric animals. There are no doubts about the efficacy of Cu in promoting growth, but its mode of action is still under debate. One of possible mechanisms is the inhibition of bile salt hydrolase (BSH) activity, which has an effect on the dietary utilization and metabolism of lipid.” Axel Minetto Product Manager Animine Alessandra Monteiro R&D Manager Animine

ISSUE FOCUS FEED & ADDITIVE MAGAZINE November 2022 25 al condition to promote lipid absorption is to limit the action of bacterial BSH enzyme. 2. MICROBIAL BILE SALT HYDROLYSE & FAT DIGESTIBILITY Compelling evidence has shown that inhibition of BSH activity should enhance weight gain by altering the bile acids pool, host signaling and lipid metabolism. The ability of growth promoter molecules, such as antibiotics, in promoting growth is highly correlated with the decrease in BSH activity. This mode of action has been shown for many antibiotics growth promoter (salinomycin, avilamycin, bacitracin, monensine, tylosin, …) and was associated to the reduction of intestinal BSH producers. Lactobacillus species is the major producers of this intestinal enzyme, but Clostridium, Listeria, and Staphylococcus are also identified as BSH producers. Recently, it has been demonstrated that some specific minerals sources (e.g.: copper, zinc and manganese) also have an impact on the activity of BSH by two mechanisms: an indirect inhibition by microbiota modulation, similar to antibiotics; a direct inhibition of BSH enzyme through an interaction on its active site. Among these minerals, Cu compounds have the highest inhibition capacity, but few studies associated their growth promoter capacity to the BSH activity. 3. MONOVALENT COPPER, BILE ACID METABOLISM AND GROWTH High dietary levels of Cu are being used since long time to promote the growth of monogastric animals. There are no doubts about the efficacy of Cu in promoting growth, but its mode of action is still under debate. One of possible mechanisms is the inhibition of BSH activity, which has an effect on the dietary utilization and metabolism of lipid. This would render available more energy to the animal. A recent study conducted by Animine in the USA (at the University of Illinois) has shown that growing pigs fed 250ppm of Cu (from monovalent copper) had a higher body weight compared to a group fed 250ppm of Cu sulfate (divalent copper). One of possible explanations for this huge effect on performance is the effect of Cu on BSH activity. Indeed, this trial showed that pigs fed monovalent High Concentration Flowability Safety standards Stability Bioavailability Animal performance POTENTIATED ZINC POTENTIATED ZINC MONOVALENT COPPER HIGHLY PURIFIED MANGANESE an international and independent supplier of precision minerals , www.animine.eu